DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade

Autores
Corbalán, Valeria; Debandi, Guillermo Oscar; Scolaro, José Alejandro; Ojeda, Agustina A.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intra- and interpopulation genetic distances for all operational taxonomic units and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). We recorded only 12 of the 18 previously described species and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies, as well as with previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by Phymaturus spurcus, Phymaturus spectabilis, Phymaturus excelsus, and Phymaturus agilis), where other authors debated the identity of the species, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically isolated species such as the group formed by Phymaturus payuniae and Phymaturus nevadoi, the group formed by Phymaturus somuncurensis and Phymaturus ceii, and the group formed by Phymaturus indistinctus and Phymaturus videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.
EEA Junín
Fil: Corbalán, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Corbalán, Valeria. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina.
Fil: Debandi, Guillermo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina.
Fil: Scolaro, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Scolaro, José Alejandro. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; Argentina
Fil: Ojeda, Agustina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Ojeda, Agustina A. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina.
Fuente
Journal of Herpetology 50 (4) : 654-666. (2016)
Materia
Lagarto
ADN
Identificación
Lizards
DNA
Identification
Phymaturus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10389
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10389
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network_name_str INTA Digital (INTA)
spelling DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus CladeCorbalán, ValeriaDebandi, Guillermo OscarScolaro, José AlejandroOjeda, Agustina A.LagartoADNIdentificaciónLizardsDNAIdentificationPhymaturusUnder the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intra- and interpopulation genetic distances for all operational taxonomic units and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). We recorded only 12 of the 18 previously described species and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies, as well as with previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by Phymaturus spurcus, Phymaturus spectabilis, Phymaturus excelsus, and Phymaturus agilis), where other authors debated the identity of the species, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically isolated species such as the group formed by Phymaturus payuniae and Phymaturus nevadoi, the group formed by Phymaturus somuncurensis and Phymaturus ceii, and the group formed by Phymaturus indistinctus and Phymaturus videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.EEA JunínFil: Corbalán, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Corbalán, Valeria. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina.Fil: Debandi, Guillermo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina.Fil: Scolaro, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Scolaro, José Alejandro. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; ArgentinaFil: Ojeda, Agustina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Ojeda, Agustina A. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina.Society for the Study of Amphibians and Reptiles2021-09-29T14:30:36Z2021-09-29T14:30:36Z2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10389https://bioone.org/journals/journal-of-herpetology/volume-50/issue-4/15-104/DNA-Barcoding-of-Phymaturus-Lizards-Reveals-Conflicts-in-Species-Delimitation/10.1670/15-104.short0022-15111937-2418https://doi.org/10.1670/15-104Journal of Herpetology 50 (4) : 654-666. (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:21Zoai:localhost:20.500.12123/10389instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:21.906INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
title DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
spellingShingle DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
Corbalán, Valeria
Lagarto
ADN
Identificación
Lizards
DNA
Identification
Phymaturus
title_short DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
title_full DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
title_fullStr DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
title_full_unstemmed DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
title_sort DNA Barcoding of Phymaturus Lizards Reveals Conflicts in Species Delimitation within the patagonicus Clade
dc.creator.none.fl_str_mv Corbalán, Valeria
Debandi, Guillermo Oscar
Scolaro, José Alejandro
Ojeda, Agustina A.
author Corbalán, Valeria
author_facet Corbalán, Valeria
Debandi, Guillermo Oscar
Scolaro, José Alejandro
Ojeda, Agustina A.
author_role author
author2 Debandi, Guillermo Oscar
Scolaro, José Alejandro
Ojeda, Agustina A.
author2_role author
author
author
dc.subject.none.fl_str_mv Lagarto
ADN
Identificación
Lizards
DNA
Identification
Phymaturus
topic Lagarto
ADN
Identificación
Lizards
DNA
Identification
Phymaturus
dc.description.none.fl_txt_mv Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intra- and interpopulation genetic distances for all operational taxonomic units and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). We recorded only 12 of the 18 previously described species and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies, as well as with previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by Phymaturus spurcus, Phymaturus spectabilis, Phymaturus excelsus, and Phymaturus agilis), where other authors debated the identity of the species, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically isolated species such as the group formed by Phymaturus payuniae and Phymaturus nevadoi, the group formed by Phymaturus somuncurensis and Phymaturus ceii, and the group formed by Phymaturus indistinctus and Phymaturus videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.
EEA Junín
Fil: Corbalán, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Corbalán, Valeria. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina.
Fil: Debandi, Guillermo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina.
Fil: Scolaro, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Scolaro, José Alejandro. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; Argentina
Fil: Ojeda, Agustina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Ojeda, Agustina A. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina.
description Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidentified species, we calculated intra- and interpopulation genetic distances for all operational taxonomic units and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). We recorded only 12 of the 18 previously described species and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies, as well as with previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by Phymaturus spurcus, Phymaturus spectabilis, Phymaturus excelsus, and Phymaturus agilis), where other authors debated the identity of the species, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically isolated species such as the group formed by Phymaturus payuniae and Phymaturus nevadoi, the group formed by Phymaturus somuncurensis and Phymaturus ceii, and the group formed by Phymaturus indistinctus and Phymaturus videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2021-09-29T14:30:36Z
2021-09-29T14:30:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10389
https://bioone.org/journals/journal-of-herpetology/volume-50/issue-4/15-104/DNA-Barcoding-of-Phymaturus-Lizards-Reveals-Conflicts-in-Species-Delimitation/10.1670/15-104.short
0022-1511
1937-2418
https://doi.org/10.1670/15-104
url http://hdl.handle.net/20.500.12123/10389
https://bioone.org/journals/journal-of-herpetology/volume-50/issue-4/15-104/DNA-Barcoding-of-Phymaturus-Lizards-Reveals-Conflicts-in-Species-Delimitation/10.1670/15-104.short
https://doi.org/10.1670/15-104
identifier_str_mv 0022-1511
1937-2418
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for the Study of Amphibians and Reptiles
publisher.none.fl_str_mv Society for the Study of Amphibians and Reptiles
dc.source.none.fl_str_mv Journal of Herpetology 50 (4) : 654-666. (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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