DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade

Autores
Corbalán, Valeria Elizabeth; Debandi, Guillermo; Scolaro, Jose Alejandro; Ojeda, Agustina Alejandra
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I (COI) to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidenfied species, we calculated intra- and inter-population genetic distances for all operational taxonomic units (OTU) and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). Only 12 of the 18 previously described species were recovered, and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies as well as previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by P. spurcus, P. spectabilis, P. excelsus, and P. agilis), where the identity of the species was previously debated by other authors, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically-isolated species such as the group formed by P. payuniae and P. nevadoi, the group formed by P. somuncurensis and P. ceii, and the group formed by P. indistinctus and P. videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.
Fil: Corbalán, Valeria Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Debandi, Guillermo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria Junin; Argentina
Fil: Scolaro, Jose Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; Argentina
Fil: Ojeda, Agustina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Materia
Coi
Liolaemidae
Patagonia
Polymorphism
Systematic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44961
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44961
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus cladeCorbalán, Valeria ElizabethDebandi, GuillermoScolaro, Jose AlejandroOjeda, Agustina AlejandraCoiLiolaemidaePatagoniaPolymorphismSystematichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I (COI) to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidenfied species, we calculated intra- and inter-population genetic distances for all operational taxonomic units (OTU) and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). Only 12 of the 18 previously described species were recovered, and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies as well as previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by P. spurcus, P. spectabilis, P. excelsus, and P. agilis), where the identity of the species was previously debated by other authors, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically-isolated species such as the group formed by P. payuniae and P. nevadoi, the group formed by P. somuncurensis and P. ceii, and the group formed by P. indistinctus and P. videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.Fil: Corbalán, Valeria Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Debandi, Guillermo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria Junin; ArgentinaFil: Scolaro, Jose Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; ArgentinaFil: Ojeda, Agustina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaSociety for the Study of Amphibians and Reptiles2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44961Corbalán, Valeria Elizabeth; Debandi, Guillermo; Scolaro, Jose Alejandro; Ojeda, Agustina Alejandra; DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade; Society for the Study of Amphibians and Reptiles; Journal of Herpetology; 50; 4; 12-2016; 654-6660022-1511CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1670/15-104info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/10.1670/15-104info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:50Zoai:ri.conicet.gov.ar:11336/44961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:50.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
title DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
spellingShingle DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
Corbalán, Valeria Elizabeth
Coi
Liolaemidae
Patagonia
Polymorphism
Systematic
title_short DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
title_full DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
title_fullStr DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
title_full_unstemmed DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
title_sort DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade
dc.creator.none.fl_str_mv Corbalán, Valeria Elizabeth
Debandi, Guillermo
Scolaro, Jose Alejandro
Ojeda, Agustina Alejandra
author Corbalán, Valeria Elizabeth
author_facet Corbalán, Valeria Elizabeth
Debandi, Guillermo
Scolaro, Jose Alejandro
Ojeda, Agustina Alejandra
author_role author
author2 Debandi, Guillermo
Scolaro, Jose Alejandro
Ojeda, Agustina Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Coi
Liolaemidae
Patagonia
Polymorphism
Systematic
topic Coi
Liolaemidae
Patagonia
Polymorphism
Systematic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I (COI) to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidenfied species, we calculated intra- and inter-population genetic distances for all operational taxonomic units (OTU) and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). Only 12 of the 18 previously described species were recovered, and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies as well as previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by P. spurcus, P. spectabilis, P. excelsus, and P. agilis), where the identity of the species was previously debated by other authors, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically-isolated species such as the group formed by P. payuniae and P. nevadoi, the group formed by P. somuncurensis and P. ceii, and the group formed by P. indistinctus and P. videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.
Fil: Corbalán, Valeria Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Debandi, Guillermo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria Junin; Argentina
Fil: Scolaro, Jose Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales; Argentina
Fil: Ojeda, Agustina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
description Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I (COI) to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidenfied species, we calculated intra- and inter-population genetic distances for all operational taxonomic units (OTU) and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). Only 12 of the 18 previously described species were recovered, and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies as well as previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by P. spurcus, P. spectabilis, P. excelsus, and P. agilis), where the identity of the species was previously debated by other authors, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically-isolated species such as the group formed by P. payuniae and P. nevadoi, the group formed by P. somuncurensis and P. ceii, and the group formed by P. indistinctus and P. videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44961
Corbalán, Valeria Elizabeth; Debandi, Guillermo; Scolaro, Jose Alejandro; Ojeda, Agustina Alejandra; DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade; Society for the Study of Amphibians and Reptiles; Journal of Herpetology; 50; 4; 12-2016; 654-666
0022-1511
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44961
identifier_str_mv Corbalán, Valeria Elizabeth; Debandi, Guillermo; Scolaro, Jose Alejandro; Ojeda, Agustina Alejandra; DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade; Society for the Study of Amphibians and Reptiles; Journal of Herpetology; 50; 4; 12-2016; 654-666
0022-1511
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1670/15-104
info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/10.1670/15-104
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for the Study of Amphibians and Reptiles
publisher.none.fl_str_mv Society for the Study of Amphibians and Reptiles
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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