Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting
- Autores
- Ferrara Muñiz, Ximena; Garcia, Elizabeth Andrea; Blanco, Federico Carlos; Garbaccio, Sergio Gabriel; Garro, Carlos Javier; Zumarraga, Martin Jose; Dellagostin, Odir A.; Trangoni, Marcos David; Marfil, Maria Jimena; Bianco, María Veronica; Abdala, Alejandro Ariel; Revelli, Javier; Bergamasco, Maria; Soutullo, Adriana; Marini, M. Rocío; Rocha, Rosana Valeria; Sánchez, Amorina; Bigi, Fabiana; Canal, Ana María; Eirin, Maria Emilia; Cataldi, Angel Adrian
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducted. Methods: Seventy-four male heifers were randomly distributed (five groups) and vaccinated subcutaneously with attenuated strains (M. bovis Δmce2 or M. bovis Δmce2-phoP), a recombinant M. bovis BCG Pasteur (BCGr) or M. bovis BCG Pasteur. Then, they cohoused with a naturally infected bTB cohort under field conditions exposed to the infection. Results: A 23% of transmission of wild-type strains was confirmed (non-vaccinated group). Strikingly, first vaccination did not induce immune response (caudal fold test and IFN-gamma release assay). However, after 74 days of exposure to bTB, animals were re-vaccinated. Although their sensitization increased throughout the trial, the vaccines did not confer significant protection, when compared to the non-vaccinated group, as demonstrated by pathology progression of lesions and confirmatory tools. Besides, the likelihood of acquiring the infection was similar in all groups compared to the non-vaccinated group (p > 0.076). Respiratory and digestive excretion of viable vaccine candidates was undetectable. To note, the group vaccinated with M. bovis Δmce2-phoP exhibited the highest proportion of animals without macroscopic lesions, compared to the one vaccinated with BCG, although this was not statistically supported. Conclusions: This highlights that further evaluation of these vaccines would not guarantee better protection. The limitations detected during the trial are discussed regarding the transmission rate of M. bovis wild-type, the imperfect test for studying sensitization, the need for a DIVA diagnosis and management conditions of the trials performed under routine husbandry conditions. Re-vaccination of likely infected bovines did not highlight a conclusive result, even suggesting a detrimental effect on those vaccinated with M. bovis BCG.
Instituto de Biotecnología
Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnología; Brasil
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Revelli, Javier. Actividad privada; Argentina
Fil: Bergamasco, Maria. Ministerio de Desarrollo Productivo de Santa Fe. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina
Fil: Soutullo, Adriana. Universidad Nacional del Litoral. Facultad de Ciencias Bioquímicas y Biológicas. Cátedra de Inmunología Básica; Argentina
Fil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina
Fil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rocha, Rosana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez, Amorina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Técnicas; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina
Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Vaccines 12 (10) : 1173 (October 2024)
- Materia
-
Bovine Tuberculosis
Bovinae
Vaccines
Live Vaccines
Field Experimentation
Tuberculosis Bovina
Vacuna
Vacuna Viva
Experimentación en Campo - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/22047
Ver los metadatos del registro completo
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Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission settingFerrara Muñiz, XimenaGarcia, Elizabeth AndreaBlanco, Federico CarlosGarbaccio, Sergio GabrielGarro, Carlos JavierZumarraga, Martin JoseDellagostin, Odir A.Trangoni, Marcos DavidMarfil, Maria JimenaBianco, María VeronicaAbdala, Alejandro ArielRevelli, JavierBergamasco, MariaSoutullo, AdrianaMarini, M. RocíoRocha, Rosana ValeriaSánchez, AmorinaBigi, FabianaCanal, Ana MaríaEirin, Maria EmiliaCataldi, Angel AdrianBovine TuberculosisBovinaeVaccinesLive VaccinesField ExperimentationTuberculosis BovinaVacunaVacuna VivaExperimentación en CampoBackground/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducted. Methods: Seventy-four male heifers were randomly distributed (five groups) and vaccinated subcutaneously with attenuated strains (M. bovis Δmce2 or M. bovis Δmce2-phoP), a recombinant M. bovis BCG Pasteur (BCGr) or M. bovis BCG Pasteur. Then, they cohoused with a naturally infected bTB cohort under field conditions exposed to the infection. Results: A 23% of transmission of wild-type strains was confirmed (non-vaccinated group). Strikingly, first vaccination did not induce immune response (caudal fold test and IFN-gamma release assay). However, after 74 days of exposure to bTB, animals were re-vaccinated. Although their sensitization increased throughout the trial, the vaccines did not confer significant protection, when compared to the non-vaccinated group, as demonstrated by pathology progression of lesions and confirmatory tools. Besides, the likelihood of acquiring the infection was similar in all groups compared to the non-vaccinated group (p > 0.076). Respiratory and digestive excretion of viable vaccine candidates was undetectable. To note, the group vaccinated with M. bovis Δmce2-phoP exhibited the highest proportion of animals without macroscopic lesions, compared to the one vaccinated with BCG, although this was not statistically supported. Conclusions: This highlights that further evaluation of these vaccines would not guarantee better protection. The limitations detected during the trial are discussed regarding the transmission rate of M. bovis wild-type, the imperfect test for studying sensitization, the need for a DIVA diagnosis and management conditions of the trials performed under routine husbandry conditions. Re-vaccination of likely infected bovines did not highlight a conclusive result, even suggesting a detrimental effect on those vaccinated with M. bovis BCG.Instituto de BiotecnologíaFil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnología; BrasilFil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Revelli, Javier. Actividad privada; ArgentinaFil: Bergamasco, Maria. Ministerio de Desarrollo Productivo de Santa Fe. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; ArgentinaFil: Soutullo, Adriana. Universidad Nacional del Litoral. Facultad de Ciencias Bioquímicas y Biológicas. Cátedra de Inmunología Básica; ArgentinaFil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; ArgentinaFil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rocha, Rosana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez, Amorina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; ArgentinaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Técnicas; ArgentinaFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; ArgentinaFil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2025-04-24T15:08:09Z2025-04-24T15:08:09Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22047https://www.mdpi.com/2076-393X/12/10/11732076-393Xhttps://doi.org/10.3390/vaccines12101173Vaccines 12 (10) : 1173 (October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicainfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. 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| dc.title.none.fl_str_mv |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| spellingShingle |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting Ferrara Muñiz, Ximena Bovine Tuberculosis Bovinae Vaccines Live Vaccines Field Experimentation Tuberculosis Bovina Vacuna Vacuna Viva Experimentación en Campo |
| title_short |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_full |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_fullStr |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_full_unstemmed |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_sort |
Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| dc.creator.none.fl_str_mv |
Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian |
| author |
Ferrara Muñiz, Ximena |
| author_facet |
Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian |
| author_role |
author |
| author2 |
Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bovine Tuberculosis Bovinae Vaccines Live Vaccines Field Experimentation Tuberculosis Bovina Vacuna Vacuna Viva Experimentación en Campo |
| topic |
Bovine Tuberculosis Bovinae Vaccines Live Vaccines Field Experimentation Tuberculosis Bovina Vacuna Vacuna Viva Experimentación en Campo |
| dc.description.none.fl_txt_mv |
Background/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducted. Methods: Seventy-four male heifers were randomly distributed (five groups) and vaccinated subcutaneously with attenuated strains (M. bovis Δmce2 or M. bovis Δmce2-phoP), a recombinant M. bovis BCG Pasteur (BCGr) or M. bovis BCG Pasteur. Then, they cohoused with a naturally infected bTB cohort under field conditions exposed to the infection. Results: A 23% of transmission of wild-type strains was confirmed (non-vaccinated group). Strikingly, first vaccination did not induce immune response (caudal fold test and IFN-gamma release assay). However, after 74 days of exposure to bTB, animals were re-vaccinated. Although their sensitization increased throughout the trial, the vaccines did not confer significant protection, when compared to the non-vaccinated group, as demonstrated by pathology progression of lesions and confirmatory tools. Besides, the likelihood of acquiring the infection was similar in all groups compared to the non-vaccinated group (p > 0.076). Respiratory and digestive excretion of viable vaccine candidates was undetectable. To note, the group vaccinated with M. bovis Δmce2-phoP exhibited the highest proportion of animals without macroscopic lesions, compared to the one vaccinated with BCG, although this was not statistically supported. Conclusions: This highlights that further evaluation of these vaccines would not guarantee better protection. The limitations detected during the trial are discussed regarding the transmission rate of M. bovis wild-type, the imperfect test for studying sensitization, the need for a DIVA diagnosis and management conditions of the trials performed under routine husbandry conditions. Re-vaccination of likely infected bovines did not highlight a conclusive result, even suggesting a detrimental effect on those vaccinated with M. bovis BCG. Instituto de Biotecnología Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnología; Brasil Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Revelli, Javier. Actividad privada; Argentina Fil: Bergamasco, Maria. Ministerio de Desarrollo Productivo de Santa Fe. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina Fil: Soutullo, Adriana. Universidad Nacional del Litoral. Facultad de Ciencias Bioquímicas y Biológicas. Cátedra de Inmunología Básica; Argentina Fil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina Fil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Rocha, Rosana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sánchez, Amorina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Técnicas; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
Background/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducted. Methods: Seventy-four male heifers were randomly distributed (five groups) and vaccinated subcutaneously with attenuated strains (M. bovis Δmce2 or M. bovis Δmce2-phoP), a recombinant M. bovis BCG Pasteur (BCGr) or M. bovis BCG Pasteur. Then, they cohoused with a naturally infected bTB cohort under field conditions exposed to the infection. Results: A 23% of transmission of wild-type strains was confirmed (non-vaccinated group). Strikingly, first vaccination did not induce immune response (caudal fold test and IFN-gamma release assay). However, after 74 days of exposure to bTB, animals were re-vaccinated. Although their sensitization increased throughout the trial, the vaccines did not confer significant protection, when compared to the non-vaccinated group, as demonstrated by pathology progression of lesions and confirmatory tools. Besides, the likelihood of acquiring the infection was similar in all groups compared to the non-vaccinated group (p > 0.076). Respiratory and digestive excretion of viable vaccine candidates was undetectable. To note, the group vaccinated with M. bovis Δmce2-phoP exhibited the highest proportion of animals without macroscopic lesions, compared to the one vaccinated with BCG, although this was not statistically supported. Conclusions: This highlights that further evaluation of these vaccines would not guarantee better protection. The limitations detected during the trial are discussed regarding the transmission rate of M. bovis wild-type, the imperfect test for studying sensitization, the need for a DIVA diagnosis and management conditions of the trials performed under routine husbandry conditions. Re-vaccination of likely infected bovines did not highlight a conclusive result, even suggesting a detrimental effect on those vaccinated with M. bovis BCG. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-10 2025-04-24T15:08:09Z 2025-04-24T15:08:09Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/22047 https://www.mdpi.com/2076-393X/12/10/1173 2076-393X https://doi.org/10.3390/vaccines12101173 |
| url |
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2076-393X |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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application/pdf |
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MDPI |
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MDPI |
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Vaccines 12 (10) : 1173 (October 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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