Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina
- Autores
- Raschia, Maria Agustina; Donzelli, María Valeria; Medus, Pablo Daniel; Cetra, Bibiana Maria; Maizon, Daniel Omar; Suarez, Victor Humberto; Pichler, Rudolf; Periasamy, Kathiravan; Poli, Mario Andres
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Selective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a complex, multifactorial trait related to host immunity. However, the mechanisms underlying host resistance and response to infection remain to be fully elucidated. In this context, the objective of this study was to provide insight into the chromosomal regions determining nematode resistance and resilience in Corriedale and resistance in Pampinta sheep breeds. A total of 170 single nucleotide polymorphisms (SNP) from 76 candidate genes for immune response were studied in 624 Corriedale and 304 Pampinta animals. Lambs underwent artificial or natural challenges with infective larvae mainly from Haemonchus contortus. Fecal egg counts, estimated breeding values for fecal egg counts, and rate of packed cell volume change and FAMACHA© score change over the challenge were used, when available, as indicators of host parasite resistance or resilience. Phenotype-genotype association studies were conducted and significance values obtained were adjusted for multiple testing errors. Eight SNPs, located on OARs 3, 6, 12, and 20, reached significance in Corriedale sheep under artificial challenge. Those SNP represent allelic variants from the MHC-Ovine Lymphocyte Antigen-DRA, two C-type lectin domain families, the Interleukin 2 receptor β, the Toll-like receptor 10, the Mannan binding lectin serine peptidase 2, and the NLR family, CARD domain containing 4 genes. On Pampinta lambs under natural challenge, we found three significant SNPs, located in the TIMP metallopeptidase inhibitor 3, the FBJ murine osteosarcoma viral oncogene homolog, and the Interleukin 20 receptor alpha genes, on OARs 3, 7, and 8, respectively. The results obtained herein confirm genomic regions previously reported as associated with nematode resistance in other sheep breeds, reinforcing their role in host response to parasites. These findings contribute to gain knowledge on parasite resistance and resilience in Corriedale sheep and report for the first time SNPs associated with resistance to gastrointestinal parasite infections in Pampinta breed.
Instituto de Genética
Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria
Fil: Donzelli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Medus, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Cetra, Bibiana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Maizon, Daniel Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Suarez, Victor Humberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Pichler, Rudolf. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria
Fil: Periasamy, Kathiravan. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria. Food and Agriculture Organization of the United Nations. Animal Production and Health Division. Animal Genetics Resources Branch; Italia
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina - Fuente
- Gene 770 : 145345 (Febrero 2021)
- Materia
-
Genes
Ovinos
Mecanismos de Defensa
Polimorfismo de un Solo Nucleótido
Resiliencia
Argentina
Sheep
Defence Mechanisms
Single Nucleotide Polymorphism
Resilience
Candidate Genes
Genes Candidatos
Raza Corriedale
Raza Pampinta - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8581
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Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in ArgentinaRaschia, Maria AgustinaDonzelli, María ValeriaMedus, Pablo DanielCetra, Bibiana MariaMaizon, Daniel OmarSuarez, Victor HumbertoPichler, RudolfPeriasamy, KathiravanPoli, Mario AndresGenesOvinosMecanismos de DefensaPolimorfismo de un Solo NucleótidoResilienciaArgentinaSheepDefence MechanismsSingle Nucleotide PolymorphismResilienceCandidate GenesGenes CandidatosRaza CorriedaleRaza PampintaSelective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a complex, multifactorial trait related to host immunity. However, the mechanisms underlying host resistance and response to infection remain to be fully elucidated. In this context, the objective of this study was to provide insight into the chromosomal regions determining nematode resistance and resilience in Corriedale and resistance in Pampinta sheep breeds. A total of 170 single nucleotide polymorphisms (SNP) from 76 candidate genes for immune response were studied in 624 Corriedale and 304 Pampinta animals. Lambs underwent artificial or natural challenges with infective larvae mainly from Haemonchus contortus. Fecal egg counts, estimated breeding values for fecal egg counts, and rate of packed cell volume change and FAMACHA© score change over the challenge were used, when available, as indicators of host parasite resistance or resilience. Phenotype-genotype association studies were conducted and significance values obtained were adjusted for multiple testing errors. Eight SNPs, located on OARs 3, 6, 12, and 20, reached significance in Corriedale sheep under artificial challenge. Those SNP represent allelic variants from the MHC-Ovine Lymphocyte Antigen-DRA, two C-type lectin domain families, the Interleukin 2 receptor β, the Toll-like receptor 10, the Mannan binding lectin serine peptidase 2, and the NLR family, CARD domain containing 4 genes. On Pampinta lambs under natural challenge, we found three significant SNPs, located in the TIMP metallopeptidase inhibitor 3, the FBJ murine osteosarcoma viral oncogene homolog, and the Interleukin 20 receptor alpha genes, on OARs 3, 7, and 8, respectively. The results obtained herein confirm genomic regions previously reported as associated with nematode resistance in other sheep breeds, reinforcing their role in host response to parasites. These findings contribute to gain knowledge on parasite resistance and resilience in Corriedale sheep and report for the first time SNPs associated with resistance to gastrointestinal parasite infections in Pampinta breed.Instituto de GenéticaFil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; AustriaFil: Donzelli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Medus, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Cetra, Bibiana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Maizon, Daniel Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Suarez, Victor Humberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Pichler, Rudolf. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; AustriaFil: Periasamy, Kathiravan. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria. Food and Agriculture Organization of the United Nations. Animal Production and Health Division. Animal Genetics Resources Branch; ItaliaFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaElsevier2021-01-08T14:48:12Z2021-01-08T14:48:12Z2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8581https://www.sciencedirect.com/science/article/pii/S03781119203101431879-0038https://doi.org/10.1016/j.gene.2020.145345Gene 770 : 145345 (Febrero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131033/AR./Genómica y biotecnología aplicada a la cría animal.info:eu-repo/semantics/restrictedAccess2025-09-04T09:48:45Zoai:localhost:20.500.12123/8581instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:45.744INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
title |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
spellingShingle |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina Raschia, Maria Agustina Genes Ovinos Mecanismos de Defensa Polimorfismo de un Solo Nucleótido Resiliencia Argentina Sheep Defence Mechanisms Single Nucleotide Polymorphism Resilience Candidate Genes Genes Candidatos Raza Corriedale Raza Pampinta |
title_short |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
title_full |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
title_fullStr |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
title_full_unstemmed |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
title_sort |
Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
dc.creator.none.fl_str_mv |
Raschia, Maria Agustina Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres |
author |
Raschia, Maria Agustina |
author_facet |
Raschia, Maria Agustina Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres |
author_role |
author |
author2 |
Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Genes Ovinos Mecanismos de Defensa Polimorfismo de un Solo Nucleótido Resiliencia Argentina Sheep Defence Mechanisms Single Nucleotide Polymorphism Resilience Candidate Genes Genes Candidatos Raza Corriedale Raza Pampinta |
topic |
Genes Ovinos Mecanismos de Defensa Polimorfismo de un Solo Nucleótido Resiliencia Argentina Sheep Defence Mechanisms Single Nucleotide Polymorphism Resilience Candidate Genes Genes Candidatos Raza Corriedale Raza Pampinta |
dc.description.none.fl_txt_mv |
Selective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a complex, multifactorial trait related to host immunity. However, the mechanisms underlying host resistance and response to infection remain to be fully elucidated. In this context, the objective of this study was to provide insight into the chromosomal regions determining nematode resistance and resilience in Corriedale and resistance in Pampinta sheep breeds. A total of 170 single nucleotide polymorphisms (SNP) from 76 candidate genes for immune response were studied in 624 Corriedale and 304 Pampinta animals. Lambs underwent artificial or natural challenges with infective larvae mainly from Haemonchus contortus. Fecal egg counts, estimated breeding values for fecal egg counts, and rate of packed cell volume change and FAMACHA© score change over the challenge were used, when available, as indicators of host parasite resistance or resilience. Phenotype-genotype association studies were conducted and significance values obtained were adjusted for multiple testing errors. Eight SNPs, located on OARs 3, 6, 12, and 20, reached significance in Corriedale sheep under artificial challenge. Those SNP represent allelic variants from the MHC-Ovine Lymphocyte Antigen-DRA, two C-type lectin domain families, the Interleukin 2 receptor β, the Toll-like receptor 10, the Mannan binding lectin serine peptidase 2, and the NLR family, CARD domain containing 4 genes. On Pampinta lambs under natural challenge, we found three significant SNPs, located in the TIMP metallopeptidase inhibitor 3, the FBJ murine osteosarcoma viral oncogene homolog, and the Interleukin 20 receptor alpha genes, on OARs 3, 7, and 8, respectively. The results obtained herein confirm genomic regions previously reported as associated with nematode resistance in other sheep breeds, reinforcing their role in host response to parasites. These findings contribute to gain knowledge on parasite resistance and resilience in Corriedale sheep and report for the first time SNPs associated with resistance to gastrointestinal parasite infections in Pampinta breed. Instituto de Genética Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Donzelli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Medus, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Cetra, Bibiana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Maizon, Daniel Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Suarez, Victor Humberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Pichler, Rudolf. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Periasamy, Kathiravan. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria. Food and Agriculture Organization of the United Nations. Animal Production and Health Division. Animal Genetics Resources Branch; Italia Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina |
description |
Selective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a complex, multifactorial trait related to host immunity. However, the mechanisms underlying host resistance and response to infection remain to be fully elucidated. In this context, the objective of this study was to provide insight into the chromosomal regions determining nematode resistance and resilience in Corriedale and resistance in Pampinta sheep breeds. A total of 170 single nucleotide polymorphisms (SNP) from 76 candidate genes for immune response were studied in 624 Corriedale and 304 Pampinta animals. Lambs underwent artificial or natural challenges with infective larvae mainly from Haemonchus contortus. Fecal egg counts, estimated breeding values for fecal egg counts, and rate of packed cell volume change and FAMACHA© score change over the challenge were used, when available, as indicators of host parasite resistance or resilience. Phenotype-genotype association studies were conducted and significance values obtained were adjusted for multiple testing errors. Eight SNPs, located on OARs 3, 6, 12, and 20, reached significance in Corriedale sheep under artificial challenge. Those SNP represent allelic variants from the MHC-Ovine Lymphocyte Antigen-DRA, two C-type lectin domain families, the Interleukin 2 receptor β, the Toll-like receptor 10, the Mannan binding lectin serine peptidase 2, and the NLR family, CARD domain containing 4 genes. On Pampinta lambs under natural challenge, we found three significant SNPs, located in the TIMP metallopeptidase inhibitor 3, the FBJ murine osteosarcoma viral oncogene homolog, and the Interleukin 20 receptor alpha genes, on OARs 3, 7, and 8, respectively. The results obtained herein confirm genomic regions previously reported as associated with nematode resistance in other sheep breeds, reinforcing their role in host response to parasites. These findings contribute to gain knowledge on parasite resistance and resilience in Corriedale sheep and report for the first time SNPs associated with resistance to gastrointestinal parasite infections in Pampinta breed. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-08T14:48:12Z 2021-01-08T14:48:12Z 2021-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/8581 https://www.sciencedirect.com/science/article/pii/S0378111920310143 1879-0038 https://doi.org/10.1016/j.gene.2020.145345 |
url |
http://hdl.handle.net/20.500.12123/8581 https://www.sciencedirect.com/science/article/pii/S0378111920310143 https://doi.org/10.1016/j.gene.2020.145345 |
identifier_str_mv |
1879-0038 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/PNBIO-1131033/AR./Genómica y biotecnología aplicada a la cría animal. |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Gene 770 : 145345 (Febrero 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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