Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins

Autores
Mann, Krin S.; Bejerman, Nicolas Esteban; Johnson, Karyn N.; Dietzgen, Ralf Georg
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV.
Inst. Patología Vegetal
Fil: Mann, Krin S. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Bejerman, Nicolás Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Johnson, Karyn N. University of Queensland. School of Biological Sciences; Australia
Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fuente
Virology 489 : 20-33. (February 2016)
Materia
Enfermedades de las Plantas
Virus de las Plantas
Proteínas
Plant Diseases
Plant Viruses
Proteins
Cytorhabdovirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1235
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1235
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network_name_str INTA Digital (INTA)
spelling Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteinsMann, Krin S.Bejerman, Nicolas EstebanJohnson, Karyn N.Dietzgen, Ralf GeorgEnfermedades de las PlantasVirus de las PlantasProteínasPlant DiseasesPlant VirusesProteinsCytorhabdovirusPlant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV.Inst. Patología VegetalFil: Mann, Krin S. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Bejerman, Nicolás Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Johnson, Karyn N. University of Queensland. School of Biological Sciences; AustraliaFil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia2017-09-18T13:19:02Z2017-09-18T13:19:02Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1235http://www.sciencedirect.com/science/article/pii/S00426822150050970042-6822https://doi.org/10.1016/j.virol.2015.11.028Virology 489 : 20-33. (February 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:22Zoai:localhost:20.500.12123/1235instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:23.153INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
title Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
spellingShingle Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
Mann, Krin S.
Enfermedades de las Plantas
Virus de las Plantas
Proteínas
Plant Diseases
Plant Viruses
Proteins
Cytorhabdovirus
title_short Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
title_full Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
title_fullStr Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
title_full_unstemmed Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
title_sort Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
dc.creator.none.fl_str_mv Mann, Krin S.
Bejerman, Nicolas Esteban
Johnson, Karyn N.
Dietzgen, Ralf Georg
author Mann, Krin S.
author_facet Mann, Krin S.
Bejerman, Nicolas Esteban
Johnson, Karyn N.
Dietzgen, Ralf Georg
author_role author
author2 Bejerman, Nicolas Esteban
Johnson, Karyn N.
Dietzgen, Ralf Georg
author2_role author
author
author
dc.subject.none.fl_str_mv Enfermedades de las Plantas
Virus de las Plantas
Proteínas
Plant Diseases
Plant Viruses
Proteins
Cytorhabdovirus
topic Enfermedades de las Plantas
Virus de las Plantas
Proteínas
Plant Diseases
Plant Viruses
Proteins
Cytorhabdovirus
dc.description.none.fl_txt_mv Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV.
Inst. Patología Vegetal
Fil: Mann, Krin S. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Bejerman, Nicolás Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Johnson, Karyn N. University of Queensland. School of Biological Sciences; Australia
Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
description Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-18T13:19:02Z
2017-09-18T13:19:02Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1235
http://www.sciencedirect.com/science/article/pii/S0042682215005097
0042-6822
https://doi.org/10.1016/j.virol.2015.11.028
url http://hdl.handle.net/20.500.12123/1235
http://www.sciencedirect.com/science/article/pii/S0042682215005097
https://doi.org/10.1016/j.virol.2015.11.028
identifier_str_mv 0042-6822
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virology 489 : 20-33. (February 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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