Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes

Autores
Sala, Juan Manuel; Wilda, Maximiliano; Miraglia, Maria Cruz; Castillo, Mariangeles; Perez Filgueira, Daniel Mariano; Freire, Teresa; Capozzo, Alejandra
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions.
EEA Mercedes
Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Sala, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Virología Humana y Animal; Argentina
Fil: Wilda, Maximiliano. Universidad Abierta Interamericana. Centro de Virología Humana y Animal; Argentina
Fil: Miraglia, María Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miraglia, María Cruz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Castillo, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castillo, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Freire, Teresa. Universidad de La República. Facultad de Medicina. Departamento de Inmunobiología. Laboratorio de Inmunomodulación y Vacunas; Uruguay
Fil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capozzo, Alejandra. Universidad Abierta Interamericana. Centro de Altos Estudios en Ciencias Humanas y de la Salud; Argentina
Fuente
Vaccines 14 (1) : 36. (January 2026)
Materia
Fasciola hepatica
Bubalus bubalis
Water Buffaloes
Animal Diseases
Antibodies
Vaccination
Aphthovirus
Immunomodulation
Búfalo de Agua
Enfermedades de los Animales
Anticuerpos
Vacunación
Virus Fiebre Aftosa
Inmunomodulación
Foot and Mouth Disease Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24960
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24960
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in BuffaloesSala, Juan ManuelWilda, MaximilianoMiraglia, Maria CruzCastillo, MariangelesPerez Filgueira, Daniel MarianoFreire, TeresaCapozzo, AlejandraFasciola hepaticaBubalus bubalisWater BuffaloesAnimal DiseasesAntibodiesVaccinationAphthovirusImmunomodulationBúfalo de AguaEnfermedades de los AnimalesAnticuerposVacunaciónVirus Fiebre AftosaInmunomodulaciónFoot and Mouth Disease VirusBackground: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions.EEA MercedesFil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Sala, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Virología Humana y Animal; ArgentinaFil: Wilda, Maximiliano. Universidad Abierta Interamericana. Centro de Virología Humana y Animal; ArgentinaFil: Miraglia, María Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miraglia, María Cruz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Castillo, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castillo, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Freire, Teresa. Universidad de La República. Facultad de Medicina. Departamento de Inmunobiología. Laboratorio de Inmunomodulación y Vacunas; UruguayFil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capozzo, Alejandra. Universidad Abierta Interamericana. Centro de Altos Estudios en Ciencias Humanas y de la Salud; ArgentinaMDPI2026-01-09T11:59:18Z2026-01-09T11:59:18Z2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24960https://www.mdpi.com/2076-393X/14/1/362076-393Xhttps://doi.org/10.3390/vaccines14010036Vaccines 14 (1) : 36. (January 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:40Zoai:localhost:20.500.12123/24960instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:40.63INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
title Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
spellingShingle Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
Sala, Juan Manuel
Fasciola hepatica
Bubalus bubalis
Water Buffaloes
Animal Diseases
Antibodies
Vaccination
Aphthovirus
Immunomodulation
Búfalo de Agua
Enfermedades de los Animales
Anticuerpos
Vacunación
Virus Fiebre Aftosa
Inmunomodulación
Foot and Mouth Disease Virus
title_short Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
title_full Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
title_fullStr Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
title_full_unstemmed Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
title_sort Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
dc.creator.none.fl_str_mv Sala, Juan Manuel
Wilda, Maximiliano
Miraglia, Maria Cruz
Castillo, Mariangeles
Perez Filgueira, Daniel Mariano
Freire, Teresa
Capozzo, Alejandra
author Sala, Juan Manuel
author_facet Sala, Juan Manuel
Wilda, Maximiliano
Miraglia, Maria Cruz
Castillo, Mariangeles
Perez Filgueira, Daniel Mariano
Freire, Teresa
Capozzo, Alejandra
author_role author
author2 Wilda, Maximiliano
Miraglia, Maria Cruz
Castillo, Mariangeles
Perez Filgueira, Daniel Mariano
Freire, Teresa
Capozzo, Alejandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fasciola hepatica
Bubalus bubalis
Water Buffaloes
Animal Diseases
Antibodies
Vaccination
Aphthovirus
Immunomodulation
Búfalo de Agua
Enfermedades de los Animales
Anticuerpos
Vacunación
Virus Fiebre Aftosa
Inmunomodulación
Foot and Mouth Disease Virus
topic Fasciola hepatica
Bubalus bubalis
Water Buffaloes
Animal Diseases
Antibodies
Vaccination
Aphthovirus
Immunomodulation
Búfalo de Agua
Enfermedades de los Animales
Anticuerpos
Vacunación
Virus Fiebre Aftosa
Inmunomodulación
Foot and Mouth Disease Virus
dc.description.none.fl_txt_mv Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions.
EEA Mercedes
Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Sala, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Virología Humana y Animal; Argentina
Fil: Wilda, Maximiliano. Universidad Abierta Interamericana. Centro de Virología Humana y Animal; Argentina
Fil: Miraglia, María Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miraglia, María Cruz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Castillo, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castillo, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Freire, Teresa. Universidad de La República. Facultad de Medicina. Departamento de Inmunobiología. Laboratorio de Inmunomodulación y Vacunas; Uruguay
Fil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capozzo, Alejandra. Universidad Abierta Interamericana. Centro de Altos Estudios en Ciencias Humanas y de la Salud; Argentina
description Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions.
publishDate 2026
dc.date.none.fl_str_mv 2026-01-09T11:59:18Z
2026-01-09T11:59:18Z
2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24960
https://www.mdpi.com/2076-393X/14/1/36
2076-393X
https://doi.org/10.3390/vaccines14010036
url http://hdl.handle.net/20.500.12123/24960
https://www.mdpi.com/2076-393X/14/1/36
https://doi.org/10.3390/vaccines14010036
identifier_str_mv 2076-393X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Vaccines 14 (1) : 36. (January 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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