An insight into the sialotranscriptome and virome of Amazonian anophelines

Autores
Scarpassa, Vera Margarete; Debat, Humberto Julio; Alencar, Ronildo Baiatone; Ferreira Saraiva, José; Calvo, Eric; Arcà, Bruno; Ribeiro, José M. C.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies.
Instituto de Patología Vegetal
Fil: Scarpassa, Vera Margarete .Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Alencar, Ronildo Baiatone. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fil: Ferreira Saraiva, José. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fil: Calvo, Eric. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research; Estados Unidos
Fil: Arcà, Bruno. Sapienza University of Rome. Division of Parasitology. Department of Public Health and Infectious Diseases
Fil: Ribeiro, José M. C. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fuente
BMC genomics 20:166. (2019)
Materia
Culicidae
Malaria
Viroses
Virosis
Salivary Glands
Glándulas Salivales
Vectores
Vectors
Anopheles
Transcriptome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4644
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4644
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling An insight into the sialotranscriptome and virome of Amazonian anophelinesScarpassa, Vera MargareteDebat, Humberto JulioAlencar, Ronildo BaiatoneFerreira Saraiva, JoséCalvo, EricArcà, BrunoRibeiro, José M. C.CulicidaeMalariaVirosesVirosisSalivary GlandsGlándulas SalivalesVectoresVectorsAnophelesTranscriptomeBackground: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies.Instituto de Patología VegetalFil: Scarpassa, Vera Margarete .Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; BrasilFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Alencar, Ronildo Baiatone. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; BrasilFil: Ferreira Saraiva, José. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; BrasilFil: Calvo, Eric. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research; Estados UnidosFil: Arcà, Bruno. Sapienza University of Rome. Division of Parasitology. Department of Public Health and Infectious DiseasesFil: Ribeiro, José M. C. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; BrasilBioMed Central2019-03-18T18:47:22Z2019-03-18T18:47:22Z2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4644https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-01471-2164https://doi.org/10.1186/s12864-019-5545-0BMC genomics 20:166. (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:52Zoai:localhost:20.500.12123/4644instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:53.327INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An insight into the sialotranscriptome and virome of Amazonian anophelines
title An insight into the sialotranscriptome and virome of Amazonian anophelines
spellingShingle An insight into the sialotranscriptome and virome of Amazonian anophelines
Scarpassa, Vera Margarete
Culicidae
Malaria
Viroses
Virosis
Salivary Glands
Glándulas Salivales
Vectores
Vectors
Anopheles
Transcriptome
title_short An insight into the sialotranscriptome and virome of Amazonian anophelines
title_full An insight into the sialotranscriptome and virome of Amazonian anophelines
title_fullStr An insight into the sialotranscriptome and virome of Amazonian anophelines
title_full_unstemmed An insight into the sialotranscriptome and virome of Amazonian anophelines
title_sort An insight into the sialotranscriptome and virome of Amazonian anophelines
dc.creator.none.fl_str_mv Scarpassa, Vera Margarete
Debat, Humberto Julio
Alencar, Ronildo Baiatone
Ferreira Saraiva, José
Calvo, Eric
Arcà, Bruno
Ribeiro, José M. C.
author Scarpassa, Vera Margarete
author_facet Scarpassa, Vera Margarete
Debat, Humberto Julio
Alencar, Ronildo Baiatone
Ferreira Saraiva, José
Calvo, Eric
Arcà, Bruno
Ribeiro, José M. C.
author_role author
author2 Debat, Humberto Julio
Alencar, Ronildo Baiatone
Ferreira Saraiva, José
Calvo, Eric
Arcà, Bruno
Ribeiro, José M. C.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Culicidae
Malaria
Viroses
Virosis
Salivary Glands
Glándulas Salivales
Vectores
Vectors
Anopheles
Transcriptome
topic Culicidae
Malaria
Viroses
Virosis
Salivary Glands
Glándulas Salivales
Vectores
Vectors
Anopheles
Transcriptome
dc.description.none.fl_txt_mv Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies.
Instituto de Patología Vegetal
Fil: Scarpassa, Vera Margarete .Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Alencar, Ronildo Baiatone. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fil: Ferreira Saraiva, José. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
Fil: Calvo, Eric. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research; Estados Unidos
Fil: Arcà, Bruno. Sapienza University of Rome. Division of Parasitology. Department of Public Health and Infectious Diseases
Fil: Ribeiro, José M. C. Instituto Nacional de Pesquisas da Amazônia. Coordenação de Biodiversidade. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue; Brasil
description Background: Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and antiinflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results: In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions: Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-18T18:47:22Z
2019-03-18T18:47:22Z
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4644
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-0
1471-2164
https://doi.org/10.1186/s12864-019-5545-0
url http://hdl.handle.net/20.500.12123/4644
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5545-0
https://doi.org/10.1186/s12864-019-5545-0
identifier_str_mv 1471-2164
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv BMC genomics 20:166. (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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