Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses

Autores
De Breuil, Soledad; López, Salvador; Dottori, Carolina Andrea; Nome Docampo, Claudia; Adkins, Scott
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Groundnut ringspot virus (GRSV; Orthotospovirus arachianuli) and tomato spotted wilt virus (TSWV; Orthotospovirus tomatomaculae) pose a significant threat to agricultural crops worldwide. Managing the diseases they cause is particularly challenging due to their broad host range and transmission by thrips vectors. Early and accurate detection is crucial, especially given the limitations of conventional serological and molecular diagnostic methods. This study introduces reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a reliable alternative for detecting and identifying GRSV and TSWV in tomato, pepper, and peanut plants. Primer sets were designed to specifically amplify fragments of the nucleoprotein gene of each virus. RT-LAMP yielded negative results in healthy plants and showed no cross-reactivity with closely related orthotospoviruses or viruses from other genera. RT-LAMP detected viral RNA in total RNA samples down to 5 pg/µl, showing greater sensitivity than RT-PCR. Field testing on symptomatic plants further validated the effectiveness of this technique. This study highlights RT-LAMP as a rapid, species-specific, and highly sensitive tool for the early detection of GRSV and TSWV in economically important crops, offering a valuable approach to improving disease management. Notably, this work is the first to report the development of an RT-LAMP assay for the specific detection of GRSV.
Instituto de Patología Vegetal
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: De Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: López, Salvador. United States Department of Agriculture. Agricultural Research Service (USDA-ARS). Horticultural Research Laboratory; Estados Unidos
Fil: Dottori, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Adkins, Scott. United States Department of Agriculture. Agricultural Research Service (USDA-ARS). Horticultural Research Laboratory; Estados Unidos
Fuente
Journal of Plant Pathology 108 (1) : 763-768 (February 2026)
Materia
Tomato Spotted Wilt Virus
Pepper
Groundnuts
Pimienta
Cacahuete
Virus del Bronceado del Tomate
Orthotospovirus arachianuli
Orthotospovirus tomatomaculae
RT-LAMP
Maní
Peanuts
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25289
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spelling Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospovirusesDe Breuil, SoledadLópez, SalvadorDottori, Carolina AndreaNome Docampo, ClaudiaAdkins, ScottTomato Spotted Wilt VirusPepperGroundnutsPimientaCacahueteVirus del Bronceado del TomateOrthotospovirus arachianuliOrthotospovirus tomatomaculaeRT-LAMPManíPeanutsGroundnut ringspot virus (GRSV; Orthotospovirus arachianuli) and tomato spotted wilt virus (TSWV; Orthotospovirus tomatomaculae) pose a significant threat to agricultural crops worldwide. Managing the diseases they cause is particularly challenging due to their broad host range and transmission by thrips vectors. Early and accurate detection is crucial, especially given the limitations of conventional serological and molecular diagnostic methods. This study introduces reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a reliable alternative for detecting and identifying GRSV and TSWV in tomato, pepper, and peanut plants. Primer sets were designed to specifically amplify fragments of the nucleoprotein gene of each virus. RT-LAMP yielded negative results in healthy plants and showed no cross-reactivity with closely related orthotospoviruses or viruses from other genera. RT-LAMP detected viral RNA in total RNA samples down to 5 pg/µl, showing greater sensitivity than RT-PCR. Field testing on symptomatic plants further validated the effectiveness of this technique. This study highlights RT-LAMP as a rapid, species-specific, and highly sensitive tool for the early detection of GRSV and TSWV in economically important crops, offering a valuable approach to improving disease management. Notably, this work is the first to report the development of an RT-LAMP assay for the specific detection of GRSV.Instituto de Patología VegetalFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: De Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: López, Salvador. United States Department of Agriculture. Agricultural Research Service (USDA-ARS). Horticultural Research Laboratory; Estados UnidosFil: Dottori, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Adkins, Scott. United States Department of Agriculture. Agricultural Research Service (USDA-ARS). Horticultural Research Laboratory; Estados UnidosSpringer2026-02-25T10:59:49Z2026-02-25T10:59:49Z2026-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25289https://link.springer.com/article/10.1007/s42161-025-02033-71125-46532239-7264 (Online)https://doi.org/10.1007/s42161-025-02033-7Journal of Plant Pathology 108 (1) : 763-768 (February 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I074, Bases ecológicas y epidemiológicas para el diseño de estrategias de manejo de plagas agrícolas y forestalesinfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:43Zoai:localhost:20.500.12123/25289instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:43.208INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
title Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
spellingShingle Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
De Breuil, Soledad
Tomato Spotted Wilt Virus
Pepper
Groundnuts
Pimienta
Cacahuete
Virus del Bronceado del Tomate
Orthotospovirus arachianuli
Orthotospovirus tomatomaculae
RT-LAMP
Maní
Peanuts
title_short Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
title_full Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
title_fullStr Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
title_full_unstemmed Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
title_sort Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses
dc.creator.none.fl_str_mv De Breuil, Soledad
López, Salvador
Dottori, Carolina Andrea
Nome Docampo, Claudia
Adkins, Scott
author De Breuil, Soledad
author_facet De Breuil, Soledad
López, Salvador
Dottori, Carolina Andrea
Nome Docampo, Claudia
Adkins, Scott
author_role author
author2 López, Salvador
Dottori, Carolina Andrea
Nome Docampo, Claudia
Adkins, Scott
author2_role author
author
author
author
dc.subject.none.fl_str_mv Tomato Spotted Wilt Virus
Pepper
Groundnuts
Pimienta
Cacahuete
Virus del Bronceado del Tomate
Orthotospovirus arachianuli
Orthotospovirus tomatomaculae
RT-LAMP
Maní
Peanuts
topic Tomato Spotted Wilt Virus
Pepper
Groundnuts
Pimienta
Cacahuete
Virus del Bronceado del Tomate
Orthotospovirus arachianuli
Orthotospovirus tomatomaculae
RT-LAMP
Maní
Peanuts
dc.description.none.fl_txt_mv Groundnut ringspot virus (GRSV; Orthotospovirus arachianuli) and tomato spotted wilt virus (TSWV; Orthotospovirus tomatomaculae) pose a significant threat to agricultural crops worldwide. Managing the diseases they cause is particularly challenging due to their broad host range and transmission by thrips vectors. Early and accurate detection is crucial, especially given the limitations of conventional serological and molecular diagnostic methods. This study introduces reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a reliable alternative for detecting and identifying GRSV and TSWV in tomato, pepper, and peanut plants. Primer sets were designed to specifically amplify fragments of the nucleoprotein gene of each virus. RT-LAMP yielded negative results in healthy plants and showed no cross-reactivity with closely related orthotospoviruses or viruses from other genera. RT-LAMP detected viral RNA in total RNA samples down to 5 pg/µl, showing greater sensitivity than RT-PCR. Field testing on symptomatic plants further validated the effectiveness of this technique. This study highlights RT-LAMP as a rapid, species-specific, and highly sensitive tool for the early detection of GRSV and TSWV in economically important crops, offering a valuable approach to improving disease management. Notably, this work is the first to report the development of an RT-LAMP assay for the specific detection of GRSV.
Instituto de Patología Vegetal
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: De Breuil, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: López, Salvador. United States Department of Agriculture. Agricultural Research Service (USDA-ARS). Horticultural Research Laboratory; Estados Unidos
Fil: Dottori, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Adkins, Scott. United States Department of Agriculture. Agricultural Research Service (USDA-ARS). Horticultural Research Laboratory; Estados Unidos
description Groundnut ringspot virus (GRSV; Orthotospovirus arachianuli) and tomato spotted wilt virus (TSWV; Orthotospovirus tomatomaculae) pose a significant threat to agricultural crops worldwide. Managing the diseases they cause is particularly challenging due to their broad host range and transmission by thrips vectors. Early and accurate detection is crucial, especially given the limitations of conventional serological and molecular diagnostic methods. This study introduces reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a reliable alternative for detecting and identifying GRSV and TSWV in tomato, pepper, and peanut plants. Primer sets were designed to specifically amplify fragments of the nucleoprotein gene of each virus. RT-LAMP yielded negative results in healthy plants and showed no cross-reactivity with closely related orthotospoviruses or viruses from other genera. RT-LAMP detected viral RNA in total RNA samples down to 5 pg/µl, showing greater sensitivity than RT-PCR. Field testing on symptomatic plants further validated the effectiveness of this technique. This study highlights RT-LAMP as a rapid, species-specific, and highly sensitive tool for the early detection of GRSV and TSWV in economically important crops, offering a valuable approach to improving disease management. Notably, this work is the first to report the development of an RT-LAMP assay for the specific detection of GRSV.
publishDate 2026
dc.date.none.fl_str_mv 2026-02-25T10:59:49Z
2026-02-25T10:59:49Z
2026-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25289
https://link.springer.com/article/10.1007/s42161-025-02033-7
1125-4653
2239-7264 (Online)
https://doi.org/10.1007/s42161-025-02033-7
url http://hdl.handle.net/20.500.12123/25289
https://link.springer.com/article/10.1007/s42161-025-02033-7
https://doi.org/10.1007/s42161-025-02033-7
identifier_str_mv 1125-4653
2239-7264 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I074, Bases ecológicas y epidemiológicas para el diseño de estrategias de manejo de plagas agrícolas y forestales
info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Plant Pathology 108 (1) : 763-768 (February 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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