Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time

Autores
Pérez, Débora Jesabel; Doucette, William Joseph; Moore, Matthew Truman
Año de publicación
2021
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed to analyze morphological changes, uptake, translocation and bioaccumulation patterns in tissues of the aquatic macrophyte Typha latifolia (cattail) after long-term atrazine exposure and to determine the presence of atrazine biodegradation metabolites, desethylatrazine (DEA) and desisopropylatrazine (DIA), in tissues. Plants were hydroponically exposed to 20 μg/L atrazine (18 exposed and 18 non-exposed) for 7, 14, 21, 28, 35 and 42 days. Plants were separated into root, rhizome, stem, and lower, middle and upper leaf sections. Atrazine was analyzed by LC-MS/MS and DIA and DEA by LC-DAD. Plants showed reductions in weight (after 21 days) and transpiration (after 28 days), both symptoms of chronic phytotoxicity. The distribution of atrazine within tissues, expressed as concentration levels (μg/kg dry weight), was as follows: middle leaf (406.10 ± 71.77) = upper leaf (339.15 ± 47.60) = lower leaf (262.43 ± 7.66) = sprout (274.53 ± 58.1) > stem (38.63 ± 7.55) = root (36.00 ± 3.49) = rhizome (26.15 ± 3.96). In submerged tissues, DEA and DIA were detected at similar concentrations. In leaves, DIA was the main metabolite identified. Results indicated that atrazine was taken up from roots to shoots and induced phytotoxicity effects that reduced the translocation to shoots. Typha likely is able to biodegrade atrazine via different metabolic pathways.
EEA Balcarce
Fil: Pérez, Débora Jesabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación Para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Doucette, William Joseph. Utah State University. Utah Water Research Laboratory; Estados Unidos.
Fil: Moore, Matthew Truman. United States Department of Agriculture. Water Quality and Ecology Research Unit; Estados Unidos.
Fuente
Chemosphere 287, Part 1: 132104 (January 2022)
Materia
Herbicidas
Atrazina
Metabolitos
Bioacumulación
Transpiración
Fitotoxicidad
Herbicides
Atrazine
Metabolites
Bioaccumulation
Transpiration
Phytotoxicity
Typha
Typha Latifolia
Cattails
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10293
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10293
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spelling Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure timePérez, Débora JesabelDoucette, William JosephMoore, Matthew TrumanHerbicidasAtrazinaMetabolitosBioacumulaciónTranspiraciónFitotoxicidadHerbicidesAtrazineMetabolitesBioaccumulationTranspirationPhytotoxicityTyphaTypha LatifoliaCattailsThe extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed to analyze morphological changes, uptake, translocation and bioaccumulation patterns in tissues of the aquatic macrophyte Typha latifolia (cattail) after long-term atrazine exposure and to determine the presence of atrazine biodegradation metabolites, desethylatrazine (DEA) and desisopropylatrazine (DIA), in tissues. Plants were hydroponically exposed to 20 μg/L atrazine (18 exposed and 18 non-exposed) for 7, 14, 21, 28, 35 and 42 days. Plants were separated into root, rhizome, stem, and lower, middle and upper leaf sections. Atrazine was analyzed by LC-MS/MS and DIA and DEA by LC-DAD. Plants showed reductions in weight (after 21 days) and transpiration (after 28 days), both symptoms of chronic phytotoxicity. The distribution of atrazine within tissues, expressed as concentration levels (μg/kg dry weight), was as follows: middle leaf (406.10 ± 71.77) = upper leaf (339.15 ± 47.60) = lower leaf (262.43 ± 7.66) = sprout (274.53 ± 58.1) > stem (38.63 ± 7.55) = root (36.00 ± 3.49) = rhizome (26.15 ± 3.96). In submerged tissues, DEA and DIA were detected at similar concentrations. In leaves, DIA was the main metabolite identified. Results indicated that atrazine was taken up from roots to shoots and induced phytotoxicity effects that reduced the translocation to shoots. Typha likely is able to biodegrade atrazine via different metabolic pathways.EEA BalcarceFil: Pérez, Débora Jesabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación Para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Doucette, William Joseph. Utah State University. Utah Water Research Laboratory; Estados Unidos.Fil: Moore, Matthew Truman. United States Department of Agriculture. Water Quality and Ecology Research Unit; Estados Unidos.Elsevier2021-09-20T10:44:58Z2021-09-20T10:44:58Z2021-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10293https://www.sciencedirect.com/science/article/pii/S00456535210257650045-6535https://doi.org/10.1016/j.chemosphere.2021.132104Chemosphere 287, Part 1: 132104 (January 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repograntAgreement/INTA/2019-PD-E2-I039-002/2019-PD-E2-I039-002/AR./REMEDIACIÓN DE SUELOS Y AGUAS Y RESTAURACIÓN ECOLÓGICA DE SISTEMAS DEGRADADOS POR USO AGROPECUARIO, AGROINDUSTRIAL Y ACTIVIDADES EXTRACTIVASinfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:05Zoai:localhost:20.500.12123/10293instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:06.781INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
title Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
spellingShingle Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
Pérez, Débora Jesabel
Herbicidas
Atrazina
Metabolitos
Bioacumulación
Transpiración
Fitotoxicidad
Herbicides
Atrazine
Metabolites
Bioaccumulation
Transpiration
Phytotoxicity
Typha
Typha Latifolia
Cattails
title_short Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
title_full Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
title_fullStr Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
title_full_unstemmed Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
title_sort Atrazine uptake, translocation, bioaccumulation and biodegradation in cattail (Typha latifolia) as a function of exposure time
dc.creator.none.fl_str_mv Pérez, Débora Jesabel
Doucette, William Joseph
Moore, Matthew Truman
author Pérez, Débora Jesabel
author_facet Pérez, Débora Jesabel
Doucette, William Joseph
Moore, Matthew Truman
author_role author
author2 Doucette, William Joseph
Moore, Matthew Truman
author2_role author
author
dc.subject.none.fl_str_mv Herbicidas
Atrazina
Metabolitos
Bioacumulación
Transpiración
Fitotoxicidad
Herbicides
Atrazine
Metabolites
Bioaccumulation
Transpiration
Phytotoxicity
Typha
Typha Latifolia
Cattails
topic Herbicidas
Atrazina
Metabolitos
Bioacumulación
Transpiración
Fitotoxicidad
Herbicides
Atrazine
Metabolites
Bioaccumulation
Transpiration
Phytotoxicity
Typha
Typha Latifolia
Cattails
dc.description.none.fl_txt_mv The extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed to analyze morphological changes, uptake, translocation and bioaccumulation patterns in tissues of the aquatic macrophyte Typha latifolia (cattail) after long-term atrazine exposure and to determine the presence of atrazine biodegradation metabolites, desethylatrazine (DEA) and desisopropylatrazine (DIA), in tissues. Plants were hydroponically exposed to 20 μg/L atrazine (18 exposed and 18 non-exposed) for 7, 14, 21, 28, 35 and 42 days. Plants were separated into root, rhizome, stem, and lower, middle and upper leaf sections. Atrazine was analyzed by LC-MS/MS and DIA and DEA by LC-DAD. Plants showed reductions in weight (after 21 days) and transpiration (after 28 days), both symptoms of chronic phytotoxicity. The distribution of atrazine within tissues, expressed as concentration levels (μg/kg dry weight), was as follows: middle leaf (406.10 ± 71.77) = upper leaf (339.15 ± 47.60) = lower leaf (262.43 ± 7.66) = sprout (274.53 ± 58.1) > stem (38.63 ± 7.55) = root (36.00 ± 3.49) = rhizome (26.15 ± 3.96). In submerged tissues, DEA and DIA were detected at similar concentrations. In leaves, DIA was the main metabolite identified. Results indicated that atrazine was taken up from roots to shoots and induced phytotoxicity effects that reduced the translocation to shoots. Typha likely is able to biodegrade atrazine via different metabolic pathways.
EEA Balcarce
Fil: Pérez, Débora Jesabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación Para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Doucette, William Joseph. Utah State University. Utah Water Research Laboratory; Estados Unidos.
Fil: Moore, Matthew Truman. United States Department of Agriculture. Water Quality and Ecology Research Unit; Estados Unidos.
description The extensive use and environmental persistence of atrazine has resulted in its ubiquitous occurrence in water resources. Some reports have described atrazine bioaccumulation and biodegradation pathways in terrestrial plants, but few have done so in aquatic macrophytes. Thus, in this study, we aimed to analyze morphological changes, uptake, translocation and bioaccumulation patterns in tissues of the aquatic macrophyte Typha latifolia (cattail) after long-term atrazine exposure and to determine the presence of atrazine biodegradation metabolites, desethylatrazine (DEA) and desisopropylatrazine (DIA), in tissues. Plants were hydroponically exposed to 20 μg/L atrazine (18 exposed and 18 non-exposed) for 7, 14, 21, 28, 35 and 42 days. Plants were separated into root, rhizome, stem, and lower, middle and upper leaf sections. Atrazine was analyzed by LC-MS/MS and DIA and DEA by LC-DAD. Plants showed reductions in weight (after 21 days) and transpiration (after 28 days), both symptoms of chronic phytotoxicity. The distribution of atrazine within tissues, expressed as concentration levels (μg/kg dry weight), was as follows: middle leaf (406.10 ± 71.77) = upper leaf (339.15 ± 47.60) = lower leaf (262.43 ± 7.66) = sprout (274.53 ± 58.1) > stem (38.63 ± 7.55) = root (36.00 ± 3.49) = rhizome (26.15 ± 3.96). In submerged tissues, DEA and DIA were detected at similar concentrations. In leaves, DIA was the main metabolite identified. Results indicated that atrazine was taken up from roots to shoots and induced phytotoxicity effects that reduced the translocation to shoots. Typha likely is able to biodegrade atrazine via different metabolic pathways.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-20T10:44:58Z
2021-09-20T10:44:58Z
2021-08-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10293
https://www.sciencedirect.com/science/article/pii/S0045653521025765
0045-6535
https://doi.org/10.1016/j.chemosphere.2021.132104
url http://hdl.handle.net/20.500.12123/10293
https://www.sciencedirect.com/science/article/pii/S0045653521025765
https://doi.org/10.1016/j.chemosphere.2021.132104
identifier_str_mv 0045-6535
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E2-I039-002/2019-PD-E2-I039-002/AR./REMEDIACIÓN DE SUELOS Y AGUAS Y RESTAURACIÓN ECOLÓGICA DE SISTEMAS DEGRADADOS POR USO AGROPECUARIO, AGROINDUSTRIAL Y ACTIVIDADES EXTRACTIVAS
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Chemosphere 287, Part 1: 132104 (January 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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