Isolation of streptomycetes causing common scab from 3-years old potato samples from South America

Autores
Rapoport, Daria; Patrmanova, T.; Kopecky, J.; Mareckova, M.; Clemente, Gladys; Salvalaggio, Andrea Eugenia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.
EEA Balcarce
Fil: Rapoport, Daria. Charles University in Prague. Faculty opf Science, República Checa. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.
Fil: Patrmanova, T. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.
Fil: Kopecky, J. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.
Fil: Mareckova, M. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Czech University of Life Sciences, Faculty of Agrobiology, Food, and Natural Resources, Department of Microbiology, República Checa.
Fil: Clemente, Gladys. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fuente
Agronomy Research 18 (S2) : 1433–1443 (2020)
Materia
Papa
Streptomyces Scabies
Enfermedades Bacterianas
América del Sur
Potatoes
Bacterial Diseases
South America
Sarna Común
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8039
Acceder
id INTADig_b4621260a38e9ae35aef41691fbcf8dc
oai_identifier_str oai:localhost:20.500.12123/8039
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Isolation of streptomycetes causing common scab from 3-years old potato samples from South AmericaRapoport, DariaPatrmanova, T.Kopecky, J.Mareckova, M.Clemente, GladysSalvalaggio, Andrea EugeniaPapaStreptomyces ScabiesEnfermedades BacterianasAmérica del SurPotatoesBacterial DiseasesSouth AmericaSarna ComúnIn this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.EEA BalcarceFil: Rapoport, Daria. Charles University in Prague. Faculty opf Science, República Checa. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.Fil: Patrmanova, T. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.Fil: Kopecky, J. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.Fil: Mareckova, M. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Czech University of Life Sciences, Faculty of Agrobiology, Food, and Natural Resources, Department of Microbiology, República Checa.Fil: Clemente, Gladys. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Estonian Agricultural University2020-10-13T17:53:55Z2020-10-13T17:53:55Z2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8039https://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf1406-894Xhttps://doi.org/10.15159/ar.20.157Agronomy Research 18 (S2) : 1433–1443 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:02Zoai:localhost:20.500.12123/8039instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:02.58INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
title Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
spellingShingle Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
Rapoport, Daria
Papa
Streptomyces Scabies
Enfermedades Bacterianas
América del Sur
Potatoes
Bacterial Diseases
South America
Sarna Común
title_short Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
title_full Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
title_fullStr Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
title_full_unstemmed Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
title_sort Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
dc.creator.none.fl_str_mv Rapoport, Daria
Patrmanova, T.
Kopecky, J.
Mareckova, M.
Clemente, Gladys
Salvalaggio, Andrea Eugenia
author Rapoport, Daria
author_facet Rapoport, Daria
Patrmanova, T.
Kopecky, J.
Mareckova, M.
Clemente, Gladys
Salvalaggio, Andrea Eugenia
author_role author
author2 Patrmanova, T.
Kopecky, J.
Mareckova, M.
Clemente, Gladys
Salvalaggio, Andrea Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Papa
Streptomyces Scabies
Enfermedades Bacterianas
América del Sur
Potatoes
Bacterial Diseases
South America
Sarna Común
topic Papa
Streptomyces Scabies
Enfermedades Bacterianas
América del Sur
Potatoes
Bacterial Diseases
South America
Sarna Común
dc.description.none.fl_txt_mv In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.
EEA Balcarce
Fil: Rapoport, Daria. Charles University in Prague. Faculty opf Science, República Checa. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.
Fil: Patrmanova, T. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.
Fil: Kopecky, J. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.
Fil: Mareckova, M. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Czech University of Life Sciences, Faculty of Agrobiology, Food, and Natural Resources, Department of Microbiology, República Checa.
Fil: Clemente, Gladys. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
description In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-13T17:53:55Z
2020-10-13T17:53:55Z
2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8039
https://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf
1406-894X
https://doi.org/10.15159/ar.20.157
url http://hdl.handle.net/20.500.12123/8039
https://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf
https://doi.org/10.15159/ar.20.157
identifier_str_mv 1406-894X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Estonian Agricultural University
publisher.none.fl_str_mv Estonian Agricultural University
dc.source.none.fl_str_mv Agronomy Research 18 (S2) : 1433–1443 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619147609112576
score 12.559606

Publicaciones similares