Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)

Autores
Maldonado, Ludmila; Hajduczyk Rutz, Jésica L.; Yañez Santos, Anahí Mara; Chiarini, Franco; Urdampilleta, Juan D.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and Vestia remain unresolved. This study describes cytogenetic traits, expands cytological records, extends phylogenetic sampling, and analyzes chromosome evolution in Cestreae through ancestral character reconstruction. Chromosome counts, karyotype analyses, and B chromosome identification were conducted using root meristem preparations stained with Giemsa. Fluorescent in situ hybridization (FISH) with rDNA probes and DAPI was used to examine rDNA distribution. Phylogenetic relationships were inferred for 23 species using four molecular markers (ITS, matK, ndhF, trnL-F) under maximum likelihood and Bayesian inference approaches. In addition to sequences from GenBank/NCBI, 14 ITS sequences were newly obtained from PCR products. Ancestral haploid chromosome numbers (n) were reconstructed using ChromEvol, while karyotype formulae and rDNA distribution patterns were inferred through stochastic character mapping (SCM) in R. All analyzed species had a chromosome number of 2n = 16, with two types of karyotype formula. Three Cestrum species possessed B chromosomes, and four distinct rDNA distribution patterns were identified. Ancestral state reconstruction suggests that 2n = 16, a karyotype formula of 7m + 1sm, and rDNA synteny (18–5.8–26S + 5S) on the sm pair #8 and terminal 18–5.8–26S sites on one m chromosome pair, may represent ancestral characteristics in Cestreae.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Maldonado, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Maldonado, Ludmila. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Hajduczyk Rutz, Jésica L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Hajduczyk Rutz, Jésica L. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Yañez Santos, Anahí Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Yañez Santos, Anahí Mara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Chiarini, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Chiarini, Franco. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Urdampilleta, Juan D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Urdampilleta, Juan D. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fuente
Taxon : 1-14 (First published: 25 September 2025)
Materia
Cestrum
Solanaceae
Phylogeny
Karyotypes
Ribosomal DNA
Filogenia
Cariotipos
ADN Ribosomal
rDNA
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24806
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network_name_str INTA Digital (INTA)
spelling Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)Maldonado, LudmilaHajduczyk Rutz, Jésica L.Yañez Santos, Anahí MaraChiarini, FrancoUrdampilleta, Juan D.CestrumSolanaceaePhylogenyKaryotypesRibosomal DNAFilogeniaCariotiposADN RibosomalrDNACestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and Vestia remain unresolved. This study describes cytogenetic traits, expands cytological records, extends phylogenetic sampling, and analyzes chromosome evolution in Cestreae through ancestral character reconstruction. Chromosome counts, karyotype analyses, and B chromosome identification were conducted using root meristem preparations stained with Giemsa. Fluorescent in situ hybridization (FISH) with rDNA probes and DAPI was used to examine rDNA distribution. Phylogenetic relationships were inferred for 23 species using four molecular markers (ITS, matK, ndhF, trnL-F) under maximum likelihood and Bayesian inference approaches. In addition to sequences from GenBank/NCBI, 14 ITS sequences were newly obtained from PCR products. Ancestral haploid chromosome numbers (n) were reconstructed using ChromEvol, while karyotype formulae and rDNA distribution patterns were inferred through stochastic character mapping (SCM) in R. All analyzed species had a chromosome number of 2n = 16, with two types of karyotype formula. Three Cestrum species possessed B chromosomes, and four distinct rDNA distribution patterns were identified. Ancestral state reconstruction suggests that 2n = 16, a karyotype formula of 7m + 1sm, and rDNA synteny (18–5.8–26S + 5S) on the sm pair #8 and terminal 18–5.8–26S sites on one m chromosome pair, may represent ancestral characteristics in Cestreae.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Maldonado, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Maldonado, Ludmila. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Hajduczyk Rutz, Jésica L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Hajduczyk Rutz, Jésica L. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Yañez Santos, Anahí Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Yañez Santos, Anahí Mara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Chiarini, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Chiarini, Franco. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Urdampilleta, Juan D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Urdampilleta, Juan D. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaWiley2025-12-30T11:21:01Z2025-12-30T11:21:01Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24806https://onlinelibrary.wiley.com/doi/full/10.1002/tax.700490040-02621996-8175https://doi.org/10.1002/tax.70049Taxon : 1-14 (First published: 25 September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:01Zoai:localhost:20.500.12123/24806instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:01.679INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
title Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
spellingShingle Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
Maldonado, Ludmila
Cestrum
Solanaceae
Phylogeny
Karyotypes
Ribosomal DNA
Filogenia
Cariotipos
ADN Ribosomal
rDNA
title_short Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
title_full Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
title_fullStr Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
title_full_unstemmed Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
title_sort Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
dc.creator.none.fl_str_mv Maldonado, Ludmila
Hajduczyk Rutz, Jésica L.
Yañez Santos, Anahí Mara
Chiarini, Franco
Urdampilleta, Juan D.
author Maldonado, Ludmila
author_facet Maldonado, Ludmila
Hajduczyk Rutz, Jésica L.
Yañez Santos, Anahí Mara
Chiarini, Franco
Urdampilleta, Juan D.
author_role author
author2 Hajduczyk Rutz, Jésica L.
Yañez Santos, Anahí Mara
Chiarini, Franco
Urdampilleta, Juan D.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cestrum
Solanaceae
Phylogeny
Karyotypes
Ribosomal DNA
Filogenia
Cariotipos
ADN Ribosomal
rDNA
topic Cestrum
Solanaceae
Phylogeny
Karyotypes
Ribosomal DNA
Filogenia
Cariotipos
ADN Ribosomal
rDNA
dc.description.none.fl_txt_mv Cestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and Vestia remain unresolved. This study describes cytogenetic traits, expands cytological records, extends phylogenetic sampling, and analyzes chromosome evolution in Cestreae through ancestral character reconstruction. Chromosome counts, karyotype analyses, and B chromosome identification were conducted using root meristem preparations stained with Giemsa. Fluorescent in situ hybridization (FISH) with rDNA probes and DAPI was used to examine rDNA distribution. Phylogenetic relationships were inferred for 23 species using four molecular markers (ITS, matK, ndhF, trnL-F) under maximum likelihood and Bayesian inference approaches. In addition to sequences from GenBank/NCBI, 14 ITS sequences were newly obtained from PCR products. Ancestral haploid chromosome numbers (n) were reconstructed using ChromEvol, while karyotype formulae and rDNA distribution patterns were inferred through stochastic character mapping (SCM) in R. All analyzed species had a chromosome number of 2n = 16, with two types of karyotype formula. Three Cestrum species possessed B chromosomes, and four distinct rDNA distribution patterns were identified. Ancestral state reconstruction suggests that 2n = 16, a karyotype formula of 7m + 1sm, and rDNA synteny (18–5.8–26S + 5S) on the sm pair #8 and terminal 18–5.8–26S sites on one m chromosome pair, may represent ancestral characteristics in Cestreae.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Maldonado, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Maldonado, Ludmila. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Hajduczyk Rutz, Jésica L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Hajduczyk Rutz, Jésica L. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Yañez Santos, Anahí Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Yañez Santos, Anahí Mara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Chiarini, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Chiarini, Franco. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Urdampilleta, Juan D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Urdampilleta, Juan D. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
description Cestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and Vestia remain unresolved. This study describes cytogenetic traits, expands cytological records, extends phylogenetic sampling, and analyzes chromosome evolution in Cestreae through ancestral character reconstruction. Chromosome counts, karyotype analyses, and B chromosome identification were conducted using root meristem preparations stained with Giemsa. Fluorescent in situ hybridization (FISH) with rDNA probes and DAPI was used to examine rDNA distribution. Phylogenetic relationships were inferred for 23 species using four molecular markers (ITS, matK, ndhF, trnL-F) under maximum likelihood and Bayesian inference approaches. In addition to sequences from GenBank/NCBI, 14 ITS sequences were newly obtained from PCR products. Ancestral haploid chromosome numbers (n) were reconstructed using ChromEvol, while karyotype formulae and rDNA distribution patterns were inferred through stochastic character mapping (SCM) in R. All analyzed species had a chromosome number of 2n = 16, with two types of karyotype formula. Three Cestrum species possessed B chromosomes, and four distinct rDNA distribution patterns were identified. Ancestral state reconstruction suggests that 2n = 16, a karyotype formula of 7m + 1sm, and rDNA synteny (18–5.8–26S + 5S) on the sm pair #8 and terminal 18–5.8–26S sites on one m chromosome pair, may represent ancestral characteristics in Cestreae.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-30T11:21:01Z
2025-12-30T11:21:01Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24806
https://onlinelibrary.wiley.com/doi/full/10.1002/tax.70049
0040-0262
1996-8175
https://doi.org/10.1002/tax.70049
url http://hdl.handle.net/20.500.12123/24806
https://onlinelibrary.wiley.com/doi/full/10.1002/tax.70049
https://doi.org/10.1002/tax.70049
identifier_str_mv 0040-0262
1996-8175
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Taxon : 1-14 (First published: 25 September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.25844

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