Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent

Autores
Carabajal Paladino, Leonale Suzel; Papeschi, Alba Graciela; Lanzavecchia, Silvia Beatriz; Cladera, Jorge Luis; Bressa, María José
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.
Instituto de Genética
Fil: Carabajal Paladino, Leonela Zusel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Bressa, Maria Jose. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fuente
European Journal of Entomology 110 (3) : 401-409 (Julio 2013)
Materia
Hymenoptera
Braconidae
Biosteres longicaudatus
Cariotipos
Heterocromatina
ADN ribosomal
Citogenética
Parasitoides
Agentes de Control Biológico
Karyotypes
Heterochromatin
Ribosomal DNA
Cytogenetics
Parasitoids
Biological Control Agents
Diachasmimorpha longicaudata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8725
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8725
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agentCarabajal Paladino, Leonale SuzelPapeschi, Alba GracielaLanzavecchia, Silvia BeatrizCladera, Jorge LuisBressa, María JoséHymenopteraBraconidaeBiosteres longicaudatusCariotiposHeterocromatinaADN ribosomalCitogenéticaParasitoidesAgentes de Control BiológicoKaryotypesHeterochromatinRibosomal DNACytogeneticsParasitoidsBiological Control AgentsDiachasmimorpha longicaudataDiachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.Instituto de GenéticaFil: Carabajal Paladino, Leonela Zusel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; ArgentinaFil: Bressa, Maria Jose. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaCzech Academy of Sciences2021-02-23T12:59:01Z2021-02-23T12:59:01Z2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8725https://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php1802-8829https://doi.org/10.14411/eje.2013.054European Journal of Entomology 110 (3) : 401-409 (Julio 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:30Zoai:localhost:20.500.12123/8725instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:30.448INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
spellingShingle Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
Carabajal Paladino, Leonale Suzel
Hymenoptera
Braconidae
Biosteres longicaudatus
Cariotipos
Heterocromatina
ADN ribosomal
Citogenética
Parasitoides
Agentes de Control Biológico
Karyotypes
Heterochromatin
Ribosomal DNA
Cytogenetics
Parasitoids
Biological Control Agents
Diachasmimorpha longicaudata
title_short Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_full Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_fullStr Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_full_unstemmed Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
title_sort Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
dc.creator.none.fl_str_mv Carabajal Paladino, Leonale Suzel
Papeschi, Alba Graciela
Lanzavecchia, Silvia Beatriz
Cladera, Jorge Luis
Bressa, María José
author Carabajal Paladino, Leonale Suzel
author_facet Carabajal Paladino, Leonale Suzel
Papeschi, Alba Graciela
Lanzavecchia, Silvia Beatriz
Cladera, Jorge Luis
Bressa, María José
author_role author
author2 Papeschi, Alba Graciela
Lanzavecchia, Silvia Beatriz
Cladera, Jorge Luis
Bressa, María José
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hymenoptera
Braconidae
Biosteres longicaudatus
Cariotipos
Heterocromatina
ADN ribosomal
Citogenética
Parasitoides
Agentes de Control Biológico
Karyotypes
Heterochromatin
Ribosomal DNA
Cytogenetics
Parasitoids
Biological Control Agents
Diachasmimorpha longicaudata
topic Hymenoptera
Braconidae
Biosteres longicaudatus
Cariotipos
Heterocromatina
ADN ribosomal
Citogenética
Parasitoides
Agentes de Control Biológico
Karyotypes
Heterochromatin
Ribosomal DNA
Cytogenetics
Parasitoids
Biological Control Agents
Diachasmimorpha longicaudata
dc.description.none.fl_txt_mv Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.
Instituto de Genética
Fil: Carabajal Paladino, Leonela Zusel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina
Fil: Bressa, Maria Jose. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
description Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
2021-02-23T12:59:01Z
2021-02-23T12:59:01Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8725
https://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php
1802-8829
https://doi.org/10.14411/eje.2013.054
url http://hdl.handle.net/20.500.12123/8725
https://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php
https://doi.org/10.14411/eje.2013.054
identifier_str_mv 1802-8829
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Czech Academy of Sciences
publisher.none.fl_str_mv Czech Academy of Sciences
dc.source.none.fl_str_mv European Journal of Entomology 110 (3) : 401-409 (Julio 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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