Grapevine virus L: a novel vitivirus in grapevine

Autores
Debat, Humberto Julio; Zavallo, Diego; Brisbane, Reid Soltero; Vončina, Darko; Almeida, Rodrigo P. P.; Blouin, Arnaud G.; Al Rwahnih, Maher; Gomez Talquenca, Gonzalo; Asurmendi, Sebastian
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Zaballo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Brisbane, Reid Soltero. Foundation Plant Services; Estados Unidos
Fil: Vončina, Darko. University of Zagreb. Faculty of Agriculture. Department of Plant Pathology; Croacia
Fil: Almeida, Rodrigo P. P. University of California. Department of Environmental Science, Policy and Management; Estados Unidos
Fil: Blouin, Arnaud G.The New Zealand Institute for Plant & Food Research Limited; Nueva Zelanda
Fil: Al Rwahnih, Maher. University of California. Department of Plant Pathology; Estados Unidos
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
European Journal of Plant Pathology 155 (1) : 319–328 (September 2019)
Materia
Vitivirus
Betaflexiviridae
Virus de las Plantas
Vid
Plant Viruses
Grapevines
Virus discovery
Itivirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Grapevine virus L: a novel vitivirus in grapevineDebat, Humberto JulioZavallo, DiegoBrisbane, Reid SolteroVončina, DarkoAlmeida, Rodrigo P. P.Blouin, Arnaud G.Al Rwahnih, MaherGomez Talquenca, GonzaloAsurmendi, SebastianVitivirusBetaflexiviridaeVirus de las PlantasVidPlant VirusesGrapevinesVirus discoveryItivirusVitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.Instituto de Patología VegetalFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Zaballo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Brisbane, Reid Soltero. Foundation Plant Services; Estados UnidosFil: Vončina, Darko. University of Zagreb. Faculty of Agriculture. Department of Plant Pathology; CroaciaFil: Almeida, Rodrigo P. P. University of California. Department of Environmental Science, Policy and Management; Estados UnidosFil: Blouin, Arnaud G.The New Zealand Institute for Plant & Food Research Limited; Nueva ZelandaFil: Al Rwahnih, Maher. University of California. Department of Plant Pathology; Estados UnidosFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2019-09-19T10:48:43Z2019-09-19T10:48:43Z2019-04-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s10658-019-01727-whttp://hdl.handle.net/20.500.12123/59070929-18731573-8469 (on line)https://doi.org/10.1007/s10658-019-01727-wEuropean Journal of Plant Pathology 155 (1) : 319–328 (September 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:38Zoai:localhost:20.500.12123/5907instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:38.471INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Grapevine virus L: a novel vitivirus in grapevine
title Grapevine virus L: a novel vitivirus in grapevine
spellingShingle Grapevine virus L: a novel vitivirus in grapevine
Debat, Humberto Julio
Vitivirus
Betaflexiviridae
Virus de las Plantas
Vid
Plant Viruses
Grapevines
Virus discovery
Itivirus
title_short Grapevine virus L: a novel vitivirus in grapevine
title_full Grapevine virus L: a novel vitivirus in grapevine
title_fullStr Grapevine virus L: a novel vitivirus in grapevine
title_full_unstemmed Grapevine virus L: a novel vitivirus in grapevine
title_sort Grapevine virus L: a novel vitivirus in grapevine
dc.creator.none.fl_str_mv Debat, Humberto Julio
Zavallo, Diego
Brisbane, Reid Soltero
Vončina, Darko
Almeida, Rodrigo P. P.
Blouin, Arnaud G.
Al Rwahnih, Maher
Gomez Talquenca, Gonzalo
Asurmendi, Sebastian
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Zavallo, Diego
Brisbane, Reid Soltero
Vončina, Darko
Almeida, Rodrigo P. P.
Blouin, Arnaud G.
Al Rwahnih, Maher
Gomez Talquenca, Gonzalo
Asurmendi, Sebastian
author_role author
author2 Zavallo, Diego
Brisbane, Reid Soltero
Vončina, Darko
Almeida, Rodrigo P. P.
Blouin, Arnaud G.
Al Rwahnih, Maher
Gomez Talquenca, Gonzalo
Asurmendi, Sebastian
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Vitivirus
Betaflexiviridae
Virus de las Plantas
Vid
Plant Viruses
Grapevines
Virus discovery
Itivirus
topic Vitivirus
Betaflexiviridae
Virus de las Plantas
Vid
Plant Viruses
Grapevines
Virus discovery
Itivirus
dc.description.none.fl_txt_mv Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Zaballo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Brisbane, Reid Soltero. Foundation Plant Services; Estados Unidos
Fil: Vončina, Darko. University of Zagreb. Faculty of Agriculture. Department of Plant Pathology; Croacia
Fil: Almeida, Rodrigo P. P. University of California. Department of Environmental Science, Policy and Management; Estados Unidos
Fil: Blouin, Arnaud G.The New Zealand Institute for Plant & Food Research Limited; Nueva Zelanda
Fil: Al Rwahnih, Maher. University of California. Department of Plant Pathology; Estados Unidos
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-19T10:48:43Z
2019-09-19T10:48:43Z
2019-04-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s10658-019-01727-w
http://hdl.handle.net/20.500.12123/5907
0929-1873
1573-8469 (on line)
https://doi.org/10.1007/s10658-019-01727-w
url https://link.springer.com/article/10.1007/s10658-019-01727-w
http://hdl.handle.net/20.500.12123/5907
https://doi.org/10.1007/s10658-019-01727-w
identifier_str_mv 0929-1873
1573-8469 (on line)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv European Journal of Plant Pathology 155 (1) : 319–328 (September 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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