Grapevine virus L: a novel vitivirus in grapevine

Autores
Debat, Humberto Julio; Zavallo, Diego; Soltero Brisbane, Reid; Voncina, Darko; Almeida, Rodrigo P.; Blouin, Arnaud G.; Al Rwahnih, Maher; Gómez Talquenca, Sebastián; Asurmendi, Sebastian
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soltero Brisbane, Reid. Foundation Plant Services; Estados Unidos
Fil: Voncina, Darko. University of Zagreb; Croacia
Fil: Almeida, Rodrigo P.. University of California at Berkeley; Estados Unidos
Fil: Blouin, Arnaud G.. No especifíca;
Fil: Al Rwahnih, Maher. University of California at Berkeley; Estados Unidos
Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
BETAFLEXIVIRIDAE
GRAPEVINE
VIRUS DISCOVERY
VITIVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147516
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147516
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Grapevine virus L: a novel vitivirus in grapevineDebat, Humberto JulioZavallo, DiegoSoltero Brisbane, ReidVoncina, DarkoAlmeida, Rodrigo P.Blouin, Arnaud G.Al Rwahnih, MaherGómez Talquenca, SebastiánAsurmendi, SebastianBETAFLEXIVIRIDAEGRAPEVINEVIRUS DISCOVERYVITIVIRUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soltero Brisbane, Reid. Foundation Plant Services; Estados UnidosFil: Voncina, Darko. University of Zagreb; CroaciaFil: Almeida, Rodrigo P.. University of California at Berkeley; Estados UnidosFil: Blouin, Arnaud G.. No especifíca;Fil: Al Rwahnih, Maher. University of California at Berkeley; Estados UnidosFil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringer2019-04-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147516Debat, Humberto Julio; Zavallo, Diego; Soltero Brisbane, Reid; Voncina, Darko; Almeida, Rodrigo P.; et al.; Grapevine virus L: a novel vitivirus in grapevine; Springer; European Journal of Plant Pathology; 155; 1; 6-4-2019; 319-3280929-1873CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10658-019-01727-winfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-019-01727-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:19Zoai:ri.conicet.gov.ar:11336/147516instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:20.244CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Grapevine virus L: a novel vitivirus in grapevine
title Grapevine virus L: a novel vitivirus in grapevine
spellingShingle Grapevine virus L: a novel vitivirus in grapevine
Debat, Humberto Julio
BETAFLEXIVIRIDAE
GRAPEVINE
VIRUS DISCOVERY
VITIVIRUS
title_short Grapevine virus L: a novel vitivirus in grapevine
title_full Grapevine virus L: a novel vitivirus in grapevine
title_fullStr Grapevine virus L: a novel vitivirus in grapevine
title_full_unstemmed Grapevine virus L: a novel vitivirus in grapevine
title_sort Grapevine virus L: a novel vitivirus in grapevine
dc.creator.none.fl_str_mv Debat, Humberto Julio
Zavallo, Diego
Soltero Brisbane, Reid
Voncina, Darko
Almeida, Rodrigo P.
Blouin, Arnaud G.
Al Rwahnih, Maher
Gómez Talquenca, Sebastián
Asurmendi, Sebastian
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Zavallo, Diego
Soltero Brisbane, Reid
Voncina, Darko
Almeida, Rodrigo P.
Blouin, Arnaud G.
Al Rwahnih, Maher
Gómez Talquenca, Sebastián
Asurmendi, Sebastian
author_role author
author2 Zavallo, Diego
Soltero Brisbane, Reid
Voncina, Darko
Almeida, Rodrigo P.
Blouin, Arnaud G.
Al Rwahnih, Maher
Gómez Talquenca, Sebastián
Asurmendi, Sebastian
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BETAFLEXIVIRIDAE
GRAPEVINE
VIRUS DISCOVERY
VITIVIRUS
topic BETAFLEXIVIRIDAE
GRAPEVINE
VIRUS DISCOVERY
VITIVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soltero Brisbane, Reid. Foundation Plant Services; Estados Unidos
Fil: Voncina, Darko. University of Zagreb; Croacia
Fil: Almeida, Rodrigo P.. University of California at Berkeley; Estados Unidos
Fil: Blouin, Arnaud G.. No especifíca;
Fil: Al Rwahnih, Maher. University of California at Berkeley; Estados Unidos
Fil: Gómez Talquenca, Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Vitiviruses are ssRNA(+) viruses in the family Betaflexiviridae (subfamily Trivirinae). There are currently 10 ICTV recognized virus species in the genus; nevertheless, the extended use of NGS technologies is rapidly expanding their diversity and official recognition of six more have been proposed recently. Here, we present the characterization of a novel virus from grapevine, which fits the genomic architecture and evolutionary constraints to be classified within the Vitivirus genus. The detected virus sequence is 7607 nt long, including a typical genome organization of ORFs encoding a replicase (RP), a 22 kDa protein, a movement protein, a coat protein (CP) and a nucleic acid binding protein. Phylogenetic analyses based on the predicted RP and CP proteins unequivocally place the new virus within the Vitivirus genus. Multiple independent RNAseq data confirmed the presence of the detected virus in berries at diverse developmental stages. Additionally, we detected, confirmed, and assembled virus sequences from grapevine samples of distinct cultivars from America, Europe, Asia and Oceania, sharing 74.4%–97.8% nt identity, suggesting that the identified virus is widely distributed and diverse. We propose the name grapevine virus L (GVL) to the detected Vitivirus.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147516
Debat, Humberto Julio; Zavallo, Diego; Soltero Brisbane, Reid; Voncina, Darko; Almeida, Rodrigo P.; et al.; Grapevine virus L: a novel vitivirus in grapevine; Springer; European Journal of Plant Pathology; 155; 1; 6-4-2019; 319-328
0929-1873
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147516
identifier_str_mv Debat, Humberto Julio; Zavallo, Diego; Soltero Brisbane, Reid; Voncina, Darko; Almeida, Rodrigo P.; et al.; Grapevine virus L: a novel vitivirus in grapevine; Springer; European Journal of Plant Pathology; 155; 1; 6-4-2019; 319-328
0929-1873
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10658-019-01727-w
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-019-01727-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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