Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress

Autores
Escalante, Maximiliano; Vigliocco, Ana Edit; Moschen, Sebastian Nicolas; Fernandez, Paula Del Carmen; Heinz, Ruth Amelia; Garcia-Garcia, Francisco; Di Rienzo, Julio A.; Andrade, Andrea; Alemano, Sergio Gabriel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants.
Instituto de Biotecnología
Fil: Escalante, Maximiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Vigliocco, Ana E. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula Del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Heinz, Ruth Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España.
Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Andrade, Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Andrade, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fuente
Plant Molecular Biology Reporter 38 : 222–237 (2020)
Materia
Girasol
Helianthus annuus
Expresión Génica
Estrés de Sequia
Lineas Consanguíneas
Sunflowers
Gene Expression Profiling
Gene Expression
Drought Stress
Inbred Lines
Water Stress
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11725
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11725
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water StressEscalante, MaximilianoVigliocco, Ana EditMoschen, Sebastian NicolasFernandez, Paula Del CarmenHeinz, Ruth AmeliaGarcia-Garcia, FranciscoDi Rienzo, Julio A.Andrade, AndreaAlemano, Sergio GabrielGirasolHelianthus annuusExpresión GénicaEstrés de SequiaLineas ConsanguíneasSunflowersGene Expression ProfilingGene ExpressionDrought StressInbred LinesWater StressAlthough sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants.Instituto de BiotecnologíaFil: Escalante, Maximiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Vigliocco, Ana E. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Paula Del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Heinz, Ruth Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Heinz, Ruth Amelia. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España.Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Andrade, Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Andrade, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; ArgentinaSpringer2022-04-25T15:08:18Z2022-04-25T15:08:18Z2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11725https://link.springer.com/article/10.1007/s11105-020-01192-40735-96401572-9818https://doi.org/10.1007/s11105-020-01192-4Plant Molecular Biology Reporter 38 : 222–237 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:32Zoai:localhost:20.500.12123/11725instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:32.709INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
title Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
spellingShingle Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
Escalante, Maximiliano
Girasol
Helianthus annuus
Expresión Génica
Estrés de Sequia
Lineas Consanguíneas
Sunflowers
Gene Expression Profiling
Gene Expression
Drought Stress
Inbred Lines
Water Stress
title_short Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
title_full Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
title_fullStr Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
title_full_unstemmed Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
title_sort Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
dc.creator.none.fl_str_mv Escalante, Maximiliano
Vigliocco, Ana Edit
Moschen, Sebastian Nicolas
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
Garcia-Garcia, Francisco
Di Rienzo, Julio A.
Andrade, Andrea
Alemano, Sergio Gabriel
author Escalante, Maximiliano
author_facet Escalante, Maximiliano
Vigliocco, Ana Edit
Moschen, Sebastian Nicolas
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
Garcia-Garcia, Francisco
Di Rienzo, Julio A.
Andrade, Andrea
Alemano, Sergio Gabriel
author_role author
author2 Vigliocco, Ana Edit
Moschen, Sebastian Nicolas
Fernandez, Paula Del Carmen
Heinz, Ruth Amelia
Garcia-Garcia, Francisco
Di Rienzo, Julio A.
Andrade, Andrea
Alemano, Sergio Gabriel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Girasol
Helianthus annuus
Expresión Génica
Estrés de Sequia
Lineas Consanguíneas
Sunflowers
Gene Expression Profiling
Gene Expression
Drought Stress
Inbred Lines
Water Stress
topic Girasol
Helianthus annuus
Expresión Génica
Estrés de Sequia
Lineas Consanguíneas
Sunflowers
Gene Expression Profiling
Gene Expression
Drought Stress
Inbred Lines
Water Stress
dc.description.none.fl_txt_mv Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants.
Instituto de Biotecnología
Fil: Escalante, Maximiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Vigliocco, Ana E. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Paula Del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Heinz, Ruth Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España.
Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Andrade, Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Andrade, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina
description Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
2022-04-25T15:08:18Z
2022-04-25T15:08:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11725
https://link.springer.com/article/10.1007/s11105-020-01192-4
0735-9640
1572-9818
https://doi.org/10.1007/s11105-020-01192-4
url http://hdl.handle.net/20.500.12123/11725
https://link.springer.com/article/10.1007/s11105-020-01192-4
https://doi.org/10.1007/s11105-020-01192-4
identifier_str_mv 0735-9640
1572-9818
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant Molecular Biology Reporter 38 : 222–237 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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