Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level

Autores
Rosello, Paula Luciana; Vigliocco, Ana Edit; Andrade, Andrea Mariela; Riera, Natalí Vanesa; Calafat, Mario Jose; Molas, María Lía; Alemano, Sergio Gabriel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seed germination and dormancy are tightly regulated by hormone metabolism and signaling pathway. We investigated the endogenous content of abscisic acid (ABA), its catabolites, and gibberellins (GAs), as well as the expression level of certain ABA and GAs metabolic and signaling genes in embryo of dry and imbibed cypselas of inbred sunflower (Helianthus annuus L., Asteraceae) lines: B123 (dormant) and B91 (non-dormant). Under our experimental conditions, the expression of RGL2 gene might be related to the ABA peak in B123 line at 3 h of imbibition. Indeed, RGL2 transcripts are absent in dry and early embedded cypselas of the non-dormant line B91. ABA increase was accompanied by a significant ABA-Glucosyl ester (ABA-GE) and phaseic acid (PA) (two ABA catabolites) decrease in B123 line (3 h) which indicates that ABA metabolism seems to be more active in this line, and that it would be involved in the imposition and maintenance of sunflower seed dormancy, as it has been reported for many species. Finally, an increase of bioactive GAs (GA1 and GA3) occurs at 12 h of imbibition in both lines after a decrease in ABA content. This study shows the first report about the RGL2 tissue-specific gene expression in sunflower inbred lines with contrasting dormancy level. Furthermore, our results provide evidence that ABA and GAs content and differential expression of metabolism and signaling genes would be interacting in seed dormancy regulation through a mechanism of action related to embryo itself.
Fil: Rosello, Paula Luciana. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vigliocco, Ana Edit. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Andrade, Andrea Mariela. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Riera, Natalí Vanesa. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Calafat, Mario Jose. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molas, María Lía. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alemano, Sergio Gabriel. Universidad Nacional de Río Cuarto; Argentina
Materia
DORMANCY
GENE EXPRESSION
GERMINATION
HELIANTHUS ANNUUS L.
PHYTOHORMONES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/179538
Acceder
id CONICETDig_a5a41e624f297e1a4360ce2d7dc26280
oai_identifier_str oai:ri.conicet.gov.ar:11336/179538
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy levelRosello, Paula LucianaVigliocco, Ana EditAndrade, Andrea MarielaRiera, Natalí VanesaCalafat, Mario JoseMolas, María LíaAlemano, Sergio GabrielDORMANCYGENE EXPRESSIONGERMINATIONHELIANTHUS ANNUUS L.PHYTOHORMONEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Seed germination and dormancy are tightly regulated by hormone metabolism and signaling pathway. We investigated the endogenous content of abscisic acid (ABA), its catabolites, and gibberellins (GAs), as well as the expression level of certain ABA and GAs metabolic and signaling genes in embryo of dry and imbibed cypselas of inbred sunflower (Helianthus annuus L., Asteraceae) lines: B123 (dormant) and B91 (non-dormant). Under our experimental conditions, the expression of RGL2 gene might be related to the ABA peak in B123 line at 3 h of imbibition. Indeed, RGL2 transcripts are absent in dry and early embedded cypselas of the non-dormant line B91. ABA increase was accompanied by a significant ABA-Glucosyl ester (ABA-GE) and phaseic acid (PA) (two ABA catabolites) decrease in B123 line (3 h) which indicates that ABA metabolism seems to be more active in this line, and that it would be involved in the imposition and maintenance of sunflower seed dormancy, as it has been reported for many species. Finally, an increase of bioactive GAs (GA1 and GA3) occurs at 12 h of imbibition in both lines after a decrease in ABA content. This study shows the first report about the RGL2 tissue-specific gene expression in sunflower inbred lines with contrasting dormancy level. Furthermore, our results provide evidence that ABA and GAs content and differential expression of metabolism and signaling genes would be interacting in seed dormancy regulation through a mechanism of action related to embryo itself.Fil: Rosello, Paula Luciana. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vigliocco, Ana Edit. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Andrade, Andrea Mariela. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Riera, Natalí Vanesa. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Calafat, Mario Jose. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molas, María Lía. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alemano, Sergio Gabriel. Universidad Nacional de Río Cuarto; ArgentinaElsevier France-Editions Scientifiques Medicales Elsevier2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/179538Rosello, Paula Luciana; Vigliocco, Ana Edit; Andrade, Andrea Mariela; Riera, Natalí Vanesa; Calafat, Mario Jose; et al.; Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 102; 2-2016; 133-1400981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942816300468info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2016.02.021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:46Zoai:ri.conicet.gov.ar:11336/179538instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:46.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
title Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
spellingShingle Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
Rosello, Paula Luciana
DORMANCY
GENE EXPRESSION
GERMINATION
HELIANTHUS ANNUUS L.
PHYTOHORMONES
title_short Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
title_full Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
title_fullStr Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
title_full_unstemmed Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
title_sort Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
dc.creator.none.fl_str_mv Rosello, Paula Luciana
Vigliocco, Ana Edit
Andrade, Andrea Mariela
Riera, Natalí Vanesa
Calafat, Mario Jose
Molas, María Lía
Alemano, Sergio Gabriel
author Rosello, Paula Luciana
author_facet Rosello, Paula Luciana
Vigliocco, Ana Edit
Andrade, Andrea Mariela
Riera, Natalí Vanesa
Calafat, Mario Jose
Molas, María Lía
Alemano, Sergio Gabriel
author_role author
author2 Vigliocco, Ana Edit
Andrade, Andrea Mariela
Riera, Natalí Vanesa
Calafat, Mario Jose
Molas, María Lía
Alemano, Sergio Gabriel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DORMANCY
GENE EXPRESSION
GERMINATION
HELIANTHUS ANNUUS L.
PHYTOHORMONES
topic DORMANCY
GENE EXPRESSION
GERMINATION
HELIANTHUS ANNUUS L.
PHYTOHORMONES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Seed germination and dormancy are tightly regulated by hormone metabolism and signaling pathway. We investigated the endogenous content of abscisic acid (ABA), its catabolites, and gibberellins (GAs), as well as the expression level of certain ABA and GAs metabolic and signaling genes in embryo of dry and imbibed cypselas of inbred sunflower (Helianthus annuus L., Asteraceae) lines: B123 (dormant) and B91 (non-dormant). Under our experimental conditions, the expression of RGL2 gene might be related to the ABA peak in B123 line at 3 h of imbibition. Indeed, RGL2 transcripts are absent in dry and early embedded cypselas of the non-dormant line B91. ABA increase was accompanied by a significant ABA-Glucosyl ester (ABA-GE) and phaseic acid (PA) (two ABA catabolites) decrease in B123 line (3 h) which indicates that ABA metabolism seems to be more active in this line, and that it would be involved in the imposition and maintenance of sunflower seed dormancy, as it has been reported for many species. Finally, an increase of bioactive GAs (GA1 and GA3) occurs at 12 h of imbibition in both lines after a decrease in ABA content. This study shows the first report about the RGL2 tissue-specific gene expression in sunflower inbred lines with contrasting dormancy level. Furthermore, our results provide evidence that ABA and GAs content and differential expression of metabolism and signaling genes would be interacting in seed dormancy regulation through a mechanism of action related to embryo itself.
Fil: Rosello, Paula Luciana. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vigliocco, Ana Edit. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Andrade, Andrea Mariela. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Riera, Natalí Vanesa. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Calafat, Mario Jose. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molas, María Lía. Universidad Nacional de La Pampa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alemano, Sergio Gabriel. Universidad Nacional de Río Cuarto; Argentina
description Seed germination and dormancy are tightly regulated by hormone metabolism and signaling pathway. We investigated the endogenous content of abscisic acid (ABA), its catabolites, and gibberellins (GAs), as well as the expression level of certain ABA and GAs metabolic and signaling genes in embryo of dry and imbibed cypselas of inbred sunflower (Helianthus annuus L., Asteraceae) lines: B123 (dormant) and B91 (non-dormant). Under our experimental conditions, the expression of RGL2 gene might be related to the ABA peak in B123 line at 3 h of imbibition. Indeed, RGL2 transcripts are absent in dry and early embedded cypselas of the non-dormant line B91. ABA increase was accompanied by a significant ABA-Glucosyl ester (ABA-GE) and phaseic acid (PA) (two ABA catabolites) decrease in B123 line (3 h) which indicates that ABA metabolism seems to be more active in this line, and that it would be involved in the imposition and maintenance of sunflower seed dormancy, as it has been reported for many species. Finally, an increase of bioactive GAs (GA1 and GA3) occurs at 12 h of imbibition in both lines after a decrease in ABA content. This study shows the first report about the RGL2 tissue-specific gene expression in sunflower inbred lines with contrasting dormancy level. Furthermore, our results provide evidence that ABA and GAs content and differential expression of metabolism and signaling genes would be interacting in seed dormancy regulation through a mechanism of action related to embryo itself.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/179538
Rosello, Paula Luciana; Vigliocco, Ana Edit; Andrade, Andrea Mariela; Riera, Natalí Vanesa; Calafat, Mario Jose; et al.; Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 102; 2-2016; 133-140
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/179538
identifier_str_mv Rosello, Paula Luciana; Vigliocco, Ana Edit; Andrade, Andrea Mariela; Riera, Natalí Vanesa; Calafat, Mario Jose; et al.; Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 102; 2-2016; 133-140
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942816300468
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2016.02.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-Editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-Editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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