Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs

Autores
Dominguez, Johanna Elizabeth; Martino, Florencia; Lovera, Rosario; Casanova, Natalia Andrea; Seah, Christine; Cavia, Regino; Corso, Alejandra; Melano, Roberto G.; Faccone, Diego; Fernandez Miyakawa, Mariano Enrique
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to elucidate the potential transmission of mcr-1, which confers colistin resistance, among Escherichia coli isolates from pigs, dairy cattle, and co-habiting rodents. In March 2018, 30 fecal samples were collected from three pig farms and one mixed cattle farm, and 31 cecal contents from rats (Rattus norvegicus) captured from the same four animal farms were analyzed. Results: Out of 26 mcr-1 positive E. coli isolates, 16 came from six rats, 10 from four pigs, and none from dairy cattle. The mcr-1-positive isolates from cohabiting rats and pigs were genetically unrelated, based on different XbaI-PFGE profiles. The plasmid profiles of one isolate per animal from each farm were analyzed by S1-PFGE. E. coli isolates from cohabiting rats and pigs showed plasmid bands of similar sizes (33 or 65 kb). To investigate the horizontal transfer of these plasmids between the animals, two pairs of E. coli isolates from pig farms 1 and 3 were selected for WGS analysis. Three of the isolates (EcoP3-1, EcoC2-1 from pigs, and Eco1266-6 from a rat) belonged to clonal complex 10 (CC10), while the other rat isolate (Eco1284-6) belonged to CC398 (ST398). Eco1266-6 (rat) and EcoC2-1 (pig) from cohabiting animals in pig farm 1 carried IncX4 plasmids with the mcr-1.1 variant. The plasmid sequences were almost identical (99.98% identity), both carrying the mcr-1.1/pap2 segment. pEcoC2-1 had a complete ISVsa5 insertion sequence upstream of the mcr-1 gene. Eco1284-6 (rat) and EcoP3-1 (pig) from pig farm 3 carried IncI2 plasmids with different allelic variants of mcr-1 (mcr-1.5 and mcr-1.1). Conclusions: E. coli isolates from cohabiting rats and pigs were genetically distinct, but one pair of isolates had very similar IncX4 plasmids, suggesting the potential for horizontal spread of plasmids carrying mcr genes. These findings suggest a threat of resistant E. coli spreading between cohabiting animals and into the environment. This underscores the importance of conducting integrated One-Health studies.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina
Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martino, Florencia. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; Argentina
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Casanova, Natalia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina
Fil: Casanova, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Seah, Christine. Public Health Ontario Laboratory; Canadá
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución; Argentina
Fil: Cavia, Regino. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corso, Alejandra. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio Antimicrobianos; Argentina
Fil: Melano, Roberto G. Public Health Ontario Laboratory; Canadá
Fil: Faccone, Diego. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; Argentina
Fil: Faccone, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
BMC Veterinary Research 21 : 271 (April 2025)
Materia
Antimicrobial Resistance
Resistance Genes
Colistin
Plasmids
Rats
Dairy Cattle
Swine
One Health Approach
Resistencia a los Antimicrobianos
Escherichia coli
Gen de Resistencia
Colistina
Plasmidios
Rata
Ganado de Leche
Cerdo
Rattus norvegicus
Enfoque Una Salud
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23111
Acceder
id INTADig_ae1a972aef1713500dfa26db519514d9
oai_identifier_str oai:localhost:20.500.12123/23111
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigsDominguez, Johanna ElizabethMartino, FlorenciaLovera, RosarioCasanova, Natalia AndreaSeah, ChristineCavia, ReginoCorso, AlejandraMelano, Roberto G.Faccone, DiegoFernandez Miyakawa, Mariano EnriqueAntimicrobial ResistanceResistance GenesColistinPlasmidsRatsDairy CattleSwineOne Health ApproachResistencia a los AntimicrobianosEscherichia coliGen de ResistenciaColistinaPlasmidiosRataGanado de LecheCerdoRattus norvegicusEnfoque Una SaludBackground: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to elucidate the potential transmission of mcr-1, which confers colistin resistance, among Escherichia coli isolates from pigs, dairy cattle, and co-habiting rodents. In March 2018, 30 fecal samples were collected from three pig farms and one mixed cattle farm, and 31 cecal contents from rats (Rattus norvegicus) captured from the same four animal farms were analyzed. Results: Out of 26 mcr-1 positive E. coli isolates, 16 came from six rats, 10 from four pigs, and none from dairy cattle. The mcr-1-positive isolates from cohabiting rats and pigs were genetically unrelated, based on different XbaI-PFGE profiles. The plasmid profiles of one isolate per animal from each farm were analyzed by S1-PFGE. E. coli isolates from cohabiting rats and pigs showed plasmid bands of similar sizes (33 or 65 kb). To investigate the horizontal transfer of these plasmids between the animals, two pairs of E. coli isolates from pig farms 1 and 3 were selected for WGS analysis. Three of the isolates (EcoP3-1, EcoC2-1 from pigs, and Eco1266-6 from a rat) belonged to clonal complex 10 (CC10), while the other rat isolate (Eco1284-6) belonged to CC398 (ST398). Eco1266-6 (rat) and EcoC2-1 (pig) from cohabiting animals in pig farm 1 carried IncX4 plasmids with the mcr-1.1 variant. The plasmid sequences were almost identical (99.98% identity), both carrying the mcr-1.1/pap2 segment. pEcoC2-1 had a complete ISVsa5 insertion sequence upstream of the mcr-1 gene. Eco1284-6 (rat) and EcoP3-1 (pig) from pig farm 3 carried IncI2 plasmids with different allelic variants of mcr-1 (mcr-1.5 and mcr-1.1). Conclusions: E. coli isolates from cohabiting rats and pigs were genetically distinct, but one pair of isolates had very similar IncX4 plasmids, suggesting the potential for horizontal spread of plasmids carrying mcr genes. These findings suggest a threat of resistant E. coli spreading between cohabiting animals and into the environment. This underscores the importance of conducting integrated One-Health studies.Instituto de PatobiologíaFil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; ArgentinaFil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martino, Florencia. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; ArgentinaFil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Casanova, Natalia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; ArgentinaFil: Casanova, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Seah, Christine. Public Health Ontario Laboratory; CanadáFil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución; ArgentinaFil: Cavia, Regino. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Corso, Alejandra. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio Antimicrobianos; ArgentinaFil: Melano, Roberto G. Public Health Ontario Laboratory; CanadáFil: Faccone, Diego. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; ArgentinaFil: Faccone, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioMed Central2025-07-23T10:09:42Z2025-07-23T10:09:42Z2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23111https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04665-41746-6148https://doi.org/10.1186/s12917-025-04665-4BMC Veterinary Research 21 : 271 (April 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-30T11:25:23Zoai:localhost:20.500.12123/23111instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-30 11:25:23.615INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
title Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
spellingShingle Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
Dominguez, Johanna Elizabeth
Antimicrobial Resistance
Resistance Genes
Colistin
Plasmids
Rats
Dairy Cattle
Swine
One Health Approach
Resistencia a los Antimicrobianos
Escherichia coli
Gen de Resistencia
Colistina
Plasmidios
Rata
Ganado de Leche
Cerdo
Rattus norvegicus
Enfoque Una Salud
title_short Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
title_full Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
title_fullStr Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
title_full_unstemmed Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
title_sort Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
dc.creator.none.fl_str_mv Dominguez, Johanna Elizabeth
Martino, Florencia
Lovera, Rosario
Casanova, Natalia Andrea
Seah, Christine
Cavia, Regino
Corso, Alejandra
Melano, Roberto G.
Faccone, Diego
Fernandez Miyakawa, Mariano Enrique
author Dominguez, Johanna Elizabeth
author_facet Dominguez, Johanna Elizabeth
Martino, Florencia
Lovera, Rosario
Casanova, Natalia Andrea
Seah, Christine
Cavia, Regino
Corso, Alejandra
Melano, Roberto G.
Faccone, Diego
Fernandez Miyakawa, Mariano Enrique
author_role author
author2 Martino, Florencia
Lovera, Rosario
Casanova, Natalia Andrea
Seah, Christine
Cavia, Regino
Corso, Alejandra
Melano, Roberto G.
Faccone, Diego
Fernandez Miyakawa, Mariano Enrique
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antimicrobial Resistance
Resistance Genes
Colistin
Plasmids
Rats
Dairy Cattle
Swine
One Health Approach
Resistencia a los Antimicrobianos
Escherichia coli
Gen de Resistencia
Colistina
Plasmidios
Rata
Ganado de Leche
Cerdo
Rattus norvegicus
Enfoque Una Salud
topic Antimicrobial Resistance
Resistance Genes
Colistin
Plasmids
Rats
Dairy Cattle
Swine
One Health Approach
Resistencia a los Antimicrobianos
Escherichia coli
Gen de Resistencia
Colistina
Plasmidios
Rata
Ganado de Leche
Cerdo
Rattus norvegicus
Enfoque Una Salud
dc.description.none.fl_txt_mv Background: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to elucidate the potential transmission of mcr-1, which confers colistin resistance, among Escherichia coli isolates from pigs, dairy cattle, and co-habiting rodents. In March 2018, 30 fecal samples were collected from three pig farms and one mixed cattle farm, and 31 cecal contents from rats (Rattus norvegicus) captured from the same four animal farms were analyzed. Results: Out of 26 mcr-1 positive E. coli isolates, 16 came from six rats, 10 from four pigs, and none from dairy cattle. The mcr-1-positive isolates from cohabiting rats and pigs were genetically unrelated, based on different XbaI-PFGE profiles. The plasmid profiles of one isolate per animal from each farm were analyzed by S1-PFGE. E. coli isolates from cohabiting rats and pigs showed plasmid bands of similar sizes (33 or 65 kb). To investigate the horizontal transfer of these plasmids between the animals, two pairs of E. coli isolates from pig farms 1 and 3 were selected for WGS analysis. Three of the isolates (EcoP3-1, EcoC2-1 from pigs, and Eco1266-6 from a rat) belonged to clonal complex 10 (CC10), while the other rat isolate (Eco1284-6) belonged to CC398 (ST398). Eco1266-6 (rat) and EcoC2-1 (pig) from cohabiting animals in pig farm 1 carried IncX4 plasmids with the mcr-1.1 variant. The plasmid sequences were almost identical (99.98% identity), both carrying the mcr-1.1/pap2 segment. pEcoC2-1 had a complete ISVsa5 insertion sequence upstream of the mcr-1 gene. Eco1284-6 (rat) and EcoP3-1 (pig) from pig farm 3 carried IncI2 plasmids with different allelic variants of mcr-1 (mcr-1.5 and mcr-1.1). Conclusions: E. coli isolates from cohabiting rats and pigs were genetically distinct, but one pair of isolates had very similar IncX4 plasmids, suggesting the potential for horizontal spread of plasmids carrying mcr genes. These findings suggest a threat of resistant E. coli spreading between cohabiting animals and into the environment. This underscores the importance of conducting integrated One-Health studies.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina
Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martino, Florencia. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; Argentina
Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Casanova, Natalia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina
Fil: Casanova, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Seah, Christine. Public Health Ontario Laboratory; Canadá
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución; Argentina
Fil: Cavia, Regino. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Corso, Alejandra. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio Antimicrobianos; Argentina
Fil: Melano, Roberto G. Public Health Ontario Laboratory; Canadá
Fil: Faccone, Diego. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; Argentina
Fil: Faccone, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to elucidate the potential transmission of mcr-1, which confers colistin resistance, among Escherichia coli isolates from pigs, dairy cattle, and co-habiting rodents. In March 2018, 30 fecal samples were collected from three pig farms and one mixed cattle farm, and 31 cecal contents from rats (Rattus norvegicus) captured from the same four animal farms were analyzed. Results: Out of 26 mcr-1 positive E. coli isolates, 16 came from six rats, 10 from four pigs, and none from dairy cattle. The mcr-1-positive isolates from cohabiting rats and pigs were genetically unrelated, based on different XbaI-PFGE profiles. The plasmid profiles of one isolate per animal from each farm were analyzed by S1-PFGE. E. coli isolates from cohabiting rats and pigs showed plasmid bands of similar sizes (33 or 65 kb). To investigate the horizontal transfer of these plasmids between the animals, two pairs of E. coli isolates from pig farms 1 and 3 were selected for WGS analysis. Three of the isolates (EcoP3-1, EcoC2-1 from pigs, and Eco1266-6 from a rat) belonged to clonal complex 10 (CC10), while the other rat isolate (Eco1284-6) belonged to CC398 (ST398). Eco1266-6 (rat) and EcoC2-1 (pig) from cohabiting animals in pig farm 1 carried IncX4 plasmids with the mcr-1.1 variant. The plasmid sequences were almost identical (99.98% identity), both carrying the mcr-1.1/pap2 segment. pEcoC2-1 had a complete ISVsa5 insertion sequence upstream of the mcr-1 gene. Eco1284-6 (rat) and EcoP3-1 (pig) from pig farm 3 carried IncI2 plasmids with different allelic variants of mcr-1 (mcr-1.5 and mcr-1.1). Conclusions: E. coli isolates from cohabiting rats and pigs were genetically distinct, but one pair of isolates had very similar IncX4 plasmids, suggesting the potential for horizontal spread of plasmids carrying mcr genes. These findings suggest a threat of resistant E. coli spreading between cohabiting animals and into the environment. This underscores the importance of conducting integrated One-Health studies.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-23T10:09:42Z
2025-07-23T10:09:42Z
2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23111
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04665-4
1746-6148
https://doi.org/10.1186/s12917-025-04665-4
url http://hdl.handle.net/20.500.12123/23111
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04665-4
https://doi.org/10.1186/s12917-025-04665-4
identifier_str_mv 1746-6148
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv BMC Veterinary Research 21 : 271 (April 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1847419505445175296
score 12.589754

Publicaciones similares