Topical ganciclovir reduces viral excretion in mares with equine coital exanthema

Autores
Vissani, Maria Aldana; Olguin Perglione, Cecilia; Zabal, Osvaldo Alfredo; Alvarez, Gustavo; Thiry, Etienne; Barrandeguy, Maria Edith; Parreño, Viviana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Equid alphaherpesvirus 3 (EHV-3) is the etiological agent of equine coital exanthema (ECE). Since no vaccines or antiviral therapies are available, prevention consists of clinical examination of mares and stallions before mating or semen collection and resting from breeding activities when lesions are present. However, this methodology does not identify subclinically infected animals. Ganciclovir is the most potent compound known to reduce EHV-3 replication. This study aimed to evaluate the efficacy of topical ganciclovir application to reduce EHV-3 replication in experimentally infected mares. A pilot study, following a double-blind completely randomized design, was carried out. Twenty mares were randomly divided into five groups (three treated with ganciclovir with different regimen of doses, one treated with placebo and one non-treated). Mares were experimentally infected with EHV-3 on day 0. Rectal temperature, clinical signs, and lesions were recorded. Daily perineal and vaginal swabs were evaluated by qPCR for virus detection. The antibody response was assessed by a virus neutralisation test in serum samples collected weekly. Mares experimentally infected with EHV-3 and treated with ganciclovir twice a day for 13 days showed reduced levels and duration of viral excretion and less severe lesions. The viral excretion period was reduced from 18 to nine days compared with the untreated groups. We concluded that ganciclovir had an antiviral effect on EHV-3 replication when topically administered in mares showing clinical signs of ECE. Further trials should be performed to optimize the dose of the antiviral for a definitive formulation.
Instituto de Virología
Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Alvarez, Gustavo. Argentina. Ejército. Dirección de Remonta y Veterinaria; Argentina
Fil: Thiry, Etienne. University of Liege. Faculty of Veterinary Medicine. FARAH Center. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Equine Veterinary Science : 103199 (Available online 24 July 2020)
Materia
Enfermedades de los Animales
Caballos
Yegua
Control de Enfermedades
Exantema Coital
Animal Diseases
Horses
Mares
Diseases Control
Coital Exanthema
Equine Coital Exanthema
Ganciclovir
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7653
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7653
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Topical ganciclovir reduces viral excretion in mares with equine coital exanthemaVissani, Maria AldanaOlguin Perglione, CeciliaZabal, Osvaldo AlfredoAlvarez, GustavoThiry, EtienneBarrandeguy, Maria EdithParreño, VivianaEnfermedades de los AnimalesCaballosYeguaControl de EnfermedadesExantema CoitalAnimal DiseasesHorsesMaresDiseases ControlCoital ExanthemaEquine Coital ExanthemaGanciclovirEquid alphaherpesvirus 3 (EHV-3) is the etiological agent of equine coital exanthema (ECE). Since no vaccines or antiviral therapies are available, prevention consists of clinical examination of mares and stallions before mating or semen collection and resting from breeding activities when lesions are present. However, this methodology does not identify subclinically infected animals. Ganciclovir is the most potent compound known to reduce EHV-3 replication. This study aimed to evaluate the efficacy of topical ganciclovir application to reduce EHV-3 replication in experimentally infected mares. A pilot study, following a double-blind completely randomized design, was carried out. Twenty mares were randomly divided into five groups (three treated with ganciclovir with different regimen of doses, one treated with placebo and one non-treated). Mares were experimentally infected with EHV-3 on day 0. Rectal temperature, clinical signs, and lesions were recorded. Daily perineal and vaginal swabs were evaluated by qPCR for virus detection. The antibody response was assessed by a virus neutralisation test in serum samples collected weekly. Mares experimentally infected with EHV-3 and treated with ganciclovir twice a day for 13 days showed reduced levels and duration of viral excretion and less severe lesions. The viral excretion period was reduced from 18 to nine days compared with the untreated groups. We concluded that ganciclovir had an antiviral effect on EHV-3 replication when topically administered in mares showing clinical signs of ECE. Further trials should be performed to optimize the dose of the antiviral for a definitive formulation.Instituto de VirologíaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Alvarez, Gustavo. Argentina. Ejército. Dirección de Remonta y Veterinaria; ArgentinaFil: Thiry, Etienne. University of Liege. Faculty of Veterinary Medicine. FARAH Center. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2021-08-022020-07-31T17:29:13Z2020-07-31T17:29:13Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7653https://www.sciencedirect.com/science/article/abs/pii/S07370806203029020737-0806https://doi.org/10.1016/j.jevs.2020.103199Journal of Equine Veterinary Science : 103199 (Available online 24 July 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:51Zoai:localhost:20.500.12123/7653instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:52.245INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
title Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
spellingShingle Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
Vissani, Maria Aldana
Enfermedades de los Animales
Caballos
Yegua
Control de Enfermedades
Exantema Coital
Animal Diseases
Horses
Mares
Diseases Control
Coital Exanthema
Equine Coital Exanthema
Ganciclovir
title_short Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
title_full Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
title_fullStr Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
title_full_unstemmed Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
title_sort Topical ganciclovir reduces viral excretion in mares with equine coital exanthema
dc.creator.none.fl_str_mv Vissani, Maria Aldana
Olguin Perglione, Cecilia
Zabal, Osvaldo Alfredo
Alvarez, Gustavo
Thiry, Etienne
Barrandeguy, Maria Edith
Parreño, Viviana
author Vissani, Maria Aldana
author_facet Vissani, Maria Aldana
Olguin Perglione, Cecilia
Zabal, Osvaldo Alfredo
Alvarez, Gustavo
Thiry, Etienne
Barrandeguy, Maria Edith
Parreño, Viviana
author_role author
author2 Olguin Perglione, Cecilia
Zabal, Osvaldo Alfredo
Alvarez, Gustavo
Thiry, Etienne
Barrandeguy, Maria Edith
Parreño, Viviana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Caballos
Yegua
Control de Enfermedades
Exantema Coital
Animal Diseases
Horses
Mares
Diseases Control
Coital Exanthema
Equine Coital Exanthema
Ganciclovir
topic Enfermedades de los Animales
Caballos
Yegua
Control de Enfermedades
Exantema Coital
Animal Diseases
Horses
Mares
Diseases Control
Coital Exanthema
Equine Coital Exanthema
Ganciclovir
dc.description.none.fl_txt_mv Equid alphaherpesvirus 3 (EHV-3) is the etiological agent of equine coital exanthema (ECE). Since no vaccines or antiviral therapies are available, prevention consists of clinical examination of mares and stallions before mating or semen collection and resting from breeding activities when lesions are present. However, this methodology does not identify subclinically infected animals. Ganciclovir is the most potent compound known to reduce EHV-3 replication. This study aimed to evaluate the efficacy of topical ganciclovir application to reduce EHV-3 replication in experimentally infected mares. A pilot study, following a double-blind completely randomized design, was carried out. Twenty mares were randomly divided into five groups (three treated with ganciclovir with different regimen of doses, one treated with placebo and one non-treated). Mares were experimentally infected with EHV-3 on day 0. Rectal temperature, clinical signs, and lesions were recorded. Daily perineal and vaginal swabs were evaluated by qPCR for virus detection. The antibody response was assessed by a virus neutralisation test in serum samples collected weekly. Mares experimentally infected with EHV-3 and treated with ganciclovir twice a day for 13 days showed reduced levels and duration of viral excretion and less severe lesions. The viral excretion period was reduced from 18 to nine days compared with the untreated groups. We concluded that ganciclovir had an antiviral effect on EHV-3 replication when topically administered in mares showing clinical signs of ECE. Further trials should be performed to optimize the dose of the antiviral for a definitive formulation.
Instituto de Virología
Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Alvarez, Gustavo. Argentina. Ejército. Dirección de Remonta y Veterinaria; Argentina
Fil: Thiry, Etienne. University of Liege. Faculty of Veterinary Medicine. FARAH Center. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Equid alphaherpesvirus 3 (EHV-3) is the etiological agent of equine coital exanthema (ECE). Since no vaccines or antiviral therapies are available, prevention consists of clinical examination of mares and stallions before mating or semen collection and resting from breeding activities when lesions are present. However, this methodology does not identify subclinically infected animals. Ganciclovir is the most potent compound known to reduce EHV-3 replication. This study aimed to evaluate the efficacy of topical ganciclovir application to reduce EHV-3 replication in experimentally infected mares. A pilot study, following a double-blind completely randomized design, was carried out. Twenty mares were randomly divided into five groups (three treated with ganciclovir with different regimen of doses, one treated with placebo and one non-treated). Mares were experimentally infected with EHV-3 on day 0. Rectal temperature, clinical signs, and lesions were recorded. Daily perineal and vaginal swabs were evaluated by qPCR for virus detection. The antibody response was assessed by a virus neutralisation test in serum samples collected weekly. Mares experimentally infected with EHV-3 and treated with ganciclovir twice a day for 13 days showed reduced levels and duration of viral excretion and less severe lesions. The viral excretion period was reduced from 18 to nine days compared with the untreated groups. We concluded that ganciclovir had an antiviral effect on EHV-3 replication when topically administered in mares showing clinical signs of ECE. Further trials should be performed to optimize the dose of the antiviral for a definitive formulation.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-31T17:29:13Z
2020-07-31T17:29:13Z
2020-07
info:eu-repo/date/embargoEnd/2021-08-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7653
https://www.sciencedirect.com/science/article/abs/pii/S0737080620302902
0737-0806
https://doi.org/10.1016/j.jevs.2020.103199
url http://hdl.handle.net/20.500.12123/7653
https://www.sciencedirect.com/science/article/abs/pii/S0737080620302902
https://doi.org/10.1016/j.jevs.2020.103199
identifier_str_mv 0737-0806
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Equine Veterinary Science : 103199 (Available online 24 July 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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