Redox regulation of autophagy in Arabidopsis: The different ROS effects
- Autores
- Robert, German; Enet, Alejandro; Saavedra Borelli, Laura Lucia; Lascano, Hernan Ramiro
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in Arabidopsis thaliana. Our results demonstrated that oxidative treatments with H 2 O 2 and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H 2 O 2 and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil:Robert, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Enet, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Enet, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Saavedra Borelli, Laura Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Saavedra Borelli, Laura Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina - Fuente
- Plant Physiology and Biochemistry 223 : 109800 (June 2025)
- Materia
-
Stress
Reactive Oxygen Species
Hydrogen Peroxide
Estres
Especies de Oxígeno Reactivo
Peróxido de Hidrógeno
Arabidopsis thaliana
Autophagy
Singlet Oxygen
Superoxide Radical
Redox State - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21950
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Redox regulation of autophagy in Arabidopsis: The different ROS effectsRobert, GermanEnet, AlejandroSaavedra Borelli, Laura LuciaLascano, Hernan RamiroStressReactive Oxygen SpeciesHydrogen PeroxideEstresEspecies de Oxígeno ReactivoPeróxido de HidrógenoArabidopsis thalianaAutophagySinglet OxygenSuperoxide RadicalRedox StateAutophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in Arabidopsis thaliana. Our results demonstrated that oxidative treatments with H 2 O 2 and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H 2 O 2 and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil:Robert, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Enet, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Enet, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Saavedra Borelli, Laura Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Saavedra Borelli, Laura Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaElsevier2025-04-08T12:37:40Z2025-04-08T12:37:40Z2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21950https://www.sciencedirect.com/science/article/abs/pii/S09819428250032860981-94281873-2690https://doi.org/10.1016/j.plaphy.2025.109800Plant Physiology and Biochemistry 223 : 109800 (June 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrialinfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:14Zoai:localhost:20.500.12123/21950instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:14.584INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
title |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
spellingShingle |
Redox regulation of autophagy in Arabidopsis: The different ROS effects Robert, German Stress Reactive Oxygen Species Hydrogen Peroxide Estres Especies de Oxígeno Reactivo Peróxido de Hidrógeno Arabidopsis thaliana Autophagy Singlet Oxygen Superoxide Radical Redox State |
title_short |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
title_full |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
title_fullStr |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
title_full_unstemmed |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
title_sort |
Redox regulation of autophagy in Arabidopsis: The different ROS effects |
dc.creator.none.fl_str_mv |
Robert, German Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro |
author |
Robert, German |
author_facet |
Robert, German Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro |
author_role |
author |
author2 |
Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Stress Reactive Oxygen Species Hydrogen Peroxide Estres Especies de Oxígeno Reactivo Peróxido de Hidrógeno Arabidopsis thaliana Autophagy Singlet Oxygen Superoxide Radical Redox State |
topic |
Stress Reactive Oxygen Species Hydrogen Peroxide Estres Especies de Oxígeno Reactivo Peróxido de Hidrógeno Arabidopsis thaliana Autophagy Singlet Oxygen Superoxide Radical Redox State |
dc.description.none.fl_txt_mv |
Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in Arabidopsis thaliana. Our results demonstrated that oxidative treatments with H 2 O 2 and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H 2 O 2 and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil:Robert, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Enet, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Enet, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Saavedra Borelli, Laura Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Saavedra Borelli, Laura Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina |
description |
Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in Arabidopsis thaliana. Our results demonstrated that oxidative treatments with H 2 O 2 and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H 2 O 2 and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025-04-08T12:37:40Z 2025-04-08T12:37:40Z 2025-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/21950 https://www.sciencedirect.com/science/article/abs/pii/S0981942825003286 0981-9428 1873-2690 https://doi.org/10.1016/j.plaphy.2025.109800 |
url |
http://hdl.handle.net/20.500.12123/21950 https://www.sciencedirect.com/science/article/abs/pii/S0981942825003286 https://doi.org/10.1016/j.plaphy.2025.109800 |
identifier_str_mv |
0981-9428 1873-2690 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Plant Physiology and Biochemistry 223 : 109800 (June 2025) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.559606 |