Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

Autores
Darqui, Flavia Soledad; Radonic, Laura Mabel; Beracochea, Valeria Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.
Instituto de Biotecnología
Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Darqui, Flavia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Radonic, Laura Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beracochea, Valeria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Beracochea, Valeria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Lopez Bilbao, Marisa Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontriers in Plant Science 12 : 767459. (November 2021)
Materia
Asteraceae
Helianthus annuus
Lechugas
Genética
Transferencia de Genes
Lettuces
Genetics
Gene Transfer
Girasol
Sunflower
Transgenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and LettuceDarqui, Flavia SoledadRadonic, Laura MabelBeracochea, Valeria CeciliaHopp, Horacio EstebanLopez Bilbao, Marisa GiselaAsteraceaeHelianthus annuusLechugasGenéticaTransferencia de GenesLettucesGeneticsGene TransferGirasolSunflowerTransgenesisThe Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.Instituto de BiotecnologíaFil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Darqui, Flavia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Radonic, Laura Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Beracochea, Valeria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Beracochea, Valeria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.Fil: Lopez Bilbao, Marisa Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Publishing2021-12-03T13:04:05Z2021-12-03T13:04:05Z2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10846https://www.frontiersin.org/articles/10.3389/fpls.2021.767459/full1664-462Xhttps://doi.org/10.3389/fpls.2021.767459Frontriers in Plant Science 12 : 767459. (November 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I508-001/2019-PE-E6-I508-001/AR./Diversificación de la oferta varietal de especies hortícolas de uso intensivo.info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuarioinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:11Zoai:localhost:20.500.12123/10846instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:12.024INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
spellingShingle Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
Darqui, Flavia Soledad
Asteraceae
Helianthus annuus
Lechugas
Genética
Transferencia de Genes
Lettuces
Genetics
Gene Transfer
Girasol
Sunflower
Transgenesis
title_short Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_full Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_fullStr Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_full_unstemmed Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
title_sort Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
dc.creator.none.fl_str_mv Darqui, Flavia Soledad
Radonic, Laura Mabel
Beracochea, Valeria Cecilia
Hopp, Horacio Esteban
Lopez Bilbao, Marisa Gisela
author Darqui, Flavia Soledad
author_facet Darqui, Flavia Soledad
Radonic, Laura Mabel
Beracochea, Valeria Cecilia
Hopp, Horacio Esteban
Lopez Bilbao, Marisa Gisela
author_role author
author2 Radonic, Laura Mabel
Beracochea, Valeria Cecilia
Hopp, Horacio Esteban
Lopez Bilbao, Marisa Gisela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Asteraceae
Helianthus annuus
Lechugas
Genética
Transferencia de Genes
Lettuces
Genetics
Gene Transfer
Girasol
Sunflower
Transgenesis
topic Asteraceae
Helianthus annuus
Lechugas
Genética
Transferencia de Genes
Lettuces
Genetics
Gene Transfer
Girasol
Sunflower
Transgenesis
dc.description.none.fl_txt_mv The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.
Instituto de Biotecnología
Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Darqui, Flavia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Radonic, Laura Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Radonic, Laura Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beracochea, Valeria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Beracochea, Valeria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Lopez Bilbao, Marisa Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-03T13:04:05Z
2021-12-03T13:04:05Z
2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10846
https://www.frontiersin.org/articles/10.3389/fpls.2021.767459/full
1664-462X
https://doi.org/10.3389/fpls.2021.767459
url http://hdl.handle.net/20.500.12123/10846
https://www.frontiersin.org/articles/10.3389/fpls.2021.767459/full
https://doi.org/10.3389/fpls.2021.767459
identifier_str_mv 1664-462X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I508-001/2019-PE-E6-I508-001/AR./Diversificación de la oferta varietal de especies hortícolas de uso intensivo.
info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Publishing
publisher.none.fl_str_mv Frontiers Publishing
dc.source.none.fl_str_mv Frontriers in Plant Science 12 : 767459. (November 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
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