Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage

Autores
Giacomelli, Jorge Ignacio; Weigel, Detlef; Chan, Raquel Lia; Manavella, Pablo Andrés
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MicroRNAs (miRNAs) are small 21-nucleotide RNAs that post-transcriptionally regulate gene expression. MiR396 controls leaf development by targeting GRF and bHLH transcription factors in Arabidopsis. WRKY transcription factors, unique to plants, have been identified as mediating varied stress responses. The sunflower (Helianthus annuus) HaWRKY6 is a particularly divergent WRKY gene exhibiting a putative target site for the miR396. A possible post-transcriptional regulation of HaWRKY6 by miR396 was investigated. Here, we used expression analyses, performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blots together with computational approaches to establish the regulatory interaction between HaWRKY6 and the identified sunflower miR396. Arabidopsis plants expressing a mi396-resistant version of HaWRKY6 confirmed the miRNA-dependency of the HaWRKY6 silencing. Sunflower plants exposed to high temperatures or salicylic acid presented opposite expression of HaWRKY6 and miR396. Experiments using the wildtype and miRNA-resistant versions of HaWRKY6 showed altered stress responses. Our results showed a role of the recently evolved miR396 regulation of HaWRKY6 during early responses to high temperature. Our study reveals how a miRNA that normally regulates development has been recruited for high-temperature protection in sunflower, a plant particularly well adapted to this type of stress.
Fil: Giacomelli, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Weigel, Detlef. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie; Alemania
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie; Alemania
Materia
GENE SILENCING
MICRORNA
MIR396
SUNFLOWER (HELIANTHUS ANNUUS)
WRKY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225600
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/225600
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damageGiacomelli, Jorge IgnacioWeigel, DetlefChan, Raquel LiaManavella, Pablo AndrésGENE SILENCINGMICRORNAMIR396SUNFLOWER (HELIANTHUS ANNUUS)WRKYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MicroRNAs (miRNAs) are small 21-nucleotide RNAs that post-transcriptionally regulate gene expression. MiR396 controls leaf development by targeting GRF and bHLH transcription factors in Arabidopsis. WRKY transcription factors, unique to plants, have been identified as mediating varied stress responses. The sunflower (Helianthus annuus) HaWRKY6 is a particularly divergent WRKY gene exhibiting a putative target site for the miR396. A possible post-transcriptional regulation of HaWRKY6 by miR396 was investigated. Here, we used expression analyses, performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blots together with computational approaches to establish the regulatory interaction between HaWRKY6 and the identified sunflower miR396. Arabidopsis plants expressing a mi396-resistant version of HaWRKY6 confirmed the miRNA-dependency of the HaWRKY6 silencing. Sunflower plants exposed to high temperatures or salicylic acid presented opposite expression of HaWRKY6 and miR396. Experiments using the wildtype and miRNA-resistant versions of HaWRKY6 showed altered stress responses. Our results showed a role of the recently evolved miR396 regulation of HaWRKY6 during early responses to high temperature. Our study reveals how a miRNA that normally regulates development has been recruited for high-temperature protection in sunflower, a plant particularly well adapted to this type of stress.Fil: Giacomelli, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Weigel, Detlef. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie; AlemaniaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie; AlemaniaWiley Blackwell Publishing, Inc2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225600Giacomelli, Jorge Ignacio; Weigel, Detlef; Chan, Raquel Lia; Manavella, Pablo Andrés; Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage; Wiley Blackwell Publishing, Inc; New Phytologist; 195; 4; 7-2012; 766-7730028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2012.04259.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1469-8137.2012.04259.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:50Zoai:ri.conicet.gov.ar:11336/225600instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:51.184CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
title Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
spellingShingle Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
Giacomelli, Jorge Ignacio
GENE SILENCING
MICRORNA
MIR396
SUNFLOWER (HELIANTHUS ANNUUS)
WRKY
title_short Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
title_full Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
title_fullStr Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
title_full_unstemmed Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
title_sort Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage
dc.creator.none.fl_str_mv Giacomelli, Jorge Ignacio
Weigel, Detlef
Chan, Raquel Lia
Manavella, Pablo Andrés
author Giacomelli, Jorge Ignacio
author_facet Giacomelli, Jorge Ignacio
Weigel, Detlef
Chan, Raquel Lia
Manavella, Pablo Andrés
author_role author
author2 Weigel, Detlef
Chan, Raquel Lia
Manavella, Pablo Andrés
author2_role author
author
author
dc.subject.none.fl_str_mv GENE SILENCING
MICRORNA
MIR396
SUNFLOWER (HELIANTHUS ANNUUS)
WRKY
topic GENE SILENCING
MICRORNA
MIR396
SUNFLOWER (HELIANTHUS ANNUUS)
WRKY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv MicroRNAs (miRNAs) are small 21-nucleotide RNAs that post-transcriptionally regulate gene expression. MiR396 controls leaf development by targeting GRF and bHLH transcription factors in Arabidopsis. WRKY transcription factors, unique to plants, have been identified as mediating varied stress responses. The sunflower (Helianthus annuus) HaWRKY6 is a particularly divergent WRKY gene exhibiting a putative target site for the miR396. A possible post-transcriptional regulation of HaWRKY6 by miR396 was investigated. Here, we used expression analyses, performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blots together with computational approaches to establish the regulatory interaction between HaWRKY6 and the identified sunflower miR396. Arabidopsis plants expressing a mi396-resistant version of HaWRKY6 confirmed the miRNA-dependency of the HaWRKY6 silencing. Sunflower plants exposed to high temperatures or salicylic acid presented opposite expression of HaWRKY6 and miR396. Experiments using the wildtype and miRNA-resistant versions of HaWRKY6 showed altered stress responses. Our results showed a role of the recently evolved miR396 regulation of HaWRKY6 during early responses to high temperature. Our study reveals how a miRNA that normally regulates development has been recruited for high-temperature protection in sunflower, a plant particularly well adapted to this type of stress.
Fil: Giacomelli, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Weigel, Detlef. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie; Alemania
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina. Institut Max Planck Fuer Gesellschaft. Institut Fur Entwicklungsbiolobie; Alemania
description MicroRNAs (miRNAs) are small 21-nucleotide RNAs that post-transcriptionally regulate gene expression. MiR396 controls leaf development by targeting GRF and bHLH transcription factors in Arabidopsis. WRKY transcription factors, unique to plants, have been identified as mediating varied stress responses. The sunflower (Helianthus annuus) HaWRKY6 is a particularly divergent WRKY gene exhibiting a putative target site for the miR396. A possible post-transcriptional regulation of HaWRKY6 by miR396 was investigated. Here, we used expression analyses, performed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blots together with computational approaches to establish the regulatory interaction between HaWRKY6 and the identified sunflower miR396. Arabidopsis plants expressing a mi396-resistant version of HaWRKY6 confirmed the miRNA-dependency of the HaWRKY6 silencing. Sunflower plants exposed to high temperatures or salicylic acid presented opposite expression of HaWRKY6 and miR396. Experiments using the wildtype and miRNA-resistant versions of HaWRKY6 showed altered stress responses. Our results showed a role of the recently evolved miR396 regulation of HaWRKY6 during early responses to high temperature. Our study reveals how a miRNA that normally regulates development has been recruited for high-temperature protection in sunflower, a plant particularly well adapted to this type of stress.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225600
Giacomelli, Jorge Ignacio; Weigel, Detlef; Chan, Raquel Lia; Manavella, Pablo Andrés; Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage; Wiley Blackwell Publishing, Inc; New Phytologist; 195; 4; 7-2012; 766-773
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225600
identifier_str_mv Giacomelli, Jorge Ignacio; Weigel, Detlef; Chan, Raquel Lia; Manavella, Pablo Andrés; Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage; Wiley Blackwell Publishing, Inc; New Phytologist; 195; 4; 7-2012; 766-773
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2012.04259.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1469-8137.2012.04259.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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