Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo

Autores
Ancarola, María Eugenia; Marcilla, Antonio; Herz, Michaela; Macchiaroli, Natalia; Pérez, Matías; Asurmendi, Sebastian; Brehm, Klaus; Poncini, Carolina; Rosenzvit, Mara Cecilia; Cucher, Marcela
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis. To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy. Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. Finally, we confirmed by capillary electrophoresis the presence of intravesicular RNA for both parasites and detected microRNAs by reverse transcription-PCR. This is the first report of EV secretion in cestode parasites and of an RNA secretion mechanism. These findings will provide valuable data not only for basic cestode biology but also for the rational search for new diagnostic targets.
Inst. de Biotecnología
Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Herz, Michaela. University of Würzburg, Institute of Hygiene and Microbiology; Alemania
Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Pérez, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Brehm, Klaus. University of Würzburg. Institute of Hygiene and Microbiology; Alemania
Fil: Poncini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Cucher, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fuente
International journal for parasitology 47 (10–11) : 675-686. (September 2017)
Materia
ARN
Cestoda
Platyhelminthes
Mesocestoides Corti
Echinococcus
RNA
Gusanos Planos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1457
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1457
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargoAncarola, María EugeniaMarcilla, AntonioHerz, MichaelaMacchiaroli, NataliaPérez, MatíasAsurmendi, SebastianBrehm, KlausPoncini, CarolinaRosenzvit, Mara CeciliaCucher, MarcelaARNCestodaPlatyhelminthesMesocestoides CortiEchinococcusRNAGusanos PlanosIntercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis. To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy. Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. Finally, we confirmed by capillary electrophoresis the presence of intravesicular RNA for both parasites and detected microRNAs by reverse transcription-PCR. This is the first report of EV secretion in cestode parasites and of an RNA secretion mechanism. These findings will provide valuable data not only for basic cestode biology but also for the rational search for new diagnostic targets.Inst. de BiotecnologíaFil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Herz, Michaela. University of Würzburg, Institute of Hygiene and Microbiology; AlemaniaFil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Pérez, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Brehm, Klaus. University of Würzburg. Institute of Hygiene and Microbiology; AlemaniaFil: Poncini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Cucher, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina2017-10-11T13:12:49Z2017-10-11T13:12:49Z2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1457http://www.sciencedirect.com/science/article/pii/S0020751917301984?via%3Dihub0020-7519 (Print)1879-0135 (Online)https://doi.org/10.1016/j.ijpara.2017.05.003International journal for parasitology 47 (10–11) : 675-686. (September 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:12Zoai:localhost:20.500.12123/1457instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:12.498INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
title Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
spellingShingle Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
Ancarola, María Eugenia
ARN
Cestoda
Platyhelminthes
Mesocestoides Corti
Echinococcus
RNA
Gusanos Planos
title_short Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
title_full Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
title_fullStr Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
title_full_unstemmed Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
title_sort Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
dc.creator.none.fl_str_mv Ancarola, María Eugenia
Marcilla, Antonio
Herz, Michaela
Macchiaroli, Natalia
Pérez, Matías
Asurmendi, Sebastian
Brehm, Klaus
Poncini, Carolina
Rosenzvit, Mara Cecilia
Cucher, Marcela
author Ancarola, María Eugenia
author_facet Ancarola, María Eugenia
Marcilla, Antonio
Herz, Michaela
Macchiaroli, Natalia
Pérez, Matías
Asurmendi, Sebastian
Brehm, Klaus
Poncini, Carolina
Rosenzvit, Mara Cecilia
Cucher, Marcela
author_role author
author2 Marcilla, Antonio
Herz, Michaela
Macchiaroli, Natalia
Pérez, Matías
Asurmendi, Sebastian
Brehm, Klaus
Poncini, Carolina
Rosenzvit, Mara Cecilia
Cucher, Marcela
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARN
Cestoda
Platyhelminthes
Mesocestoides Corti
Echinococcus
RNA
Gusanos Planos
topic ARN
Cestoda
Platyhelminthes
Mesocestoides Corti
Echinococcus
RNA
Gusanos Planos
dc.description.none.fl_txt_mv Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis. To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy. Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. Finally, we confirmed by capillary electrophoresis the presence of intravesicular RNA for both parasites and detected microRNAs by reverse transcription-PCR. This is the first report of EV secretion in cestode parasites and of an RNA secretion mechanism. These findings will provide valuable data not only for basic cestode biology but also for the rational search for new diagnostic targets.
Inst. de Biotecnología
Fil: Ancarola, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Herz, Michaela. University of Würzburg, Institute of Hygiene and Microbiology; Alemania
Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Pérez, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Brehm, Klaus. University of Würzburg. Institute of Hygiene and Microbiology; Alemania
Fil: Poncini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Cucher, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported in protozoans, trematodes and nematodes. Here we report that this mechanism is present in three different species of cestodes, Taenia crassiceps, Mesocestoides corti and Echinococcus multilocularis. To confirm this we determined, in vitro, the presence of EVs in culture supernatants by transmission electron microscopy. Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. Finally, we confirmed by capillary electrophoresis the presence of intravesicular RNA for both parasites and detected microRNAs by reverse transcription-PCR. This is the first report of EV secretion in cestode parasites and of an RNA secretion mechanism. These findings will provide valuable data not only for basic cestode biology but also for the rational search for new diagnostic targets.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-11T13:12:49Z
2017-10-11T13:12:49Z
2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1457
http://www.sciencedirect.com/science/article/pii/S0020751917301984?via%3Dihub
0020-7519 (Print)
1879-0135 (Online)
https://doi.org/10.1016/j.ijpara.2017.05.003
url http://hdl.handle.net/20.500.12123/1457
http://www.sciencedirect.com/science/article/pii/S0020751917301984?via%3Dihub
https://doi.org/10.1016/j.ijpara.2017.05.003
identifier_str_mv 0020-7519 (Print)
1879-0135 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv International journal for parasitology 47 (10–11) : 675-686. (September 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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