AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost
- Autores
- Vercellino, Roman Boris; Pandolfo, Claudio Ezequiel; Breccia, Gabriela; Cantamutto, Miguel Angel; Presotto, Alejandro Daniel
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- BACKGROUND: Feral radish (Raphanus sativus L.) is a problematic weed that has become resistant to acetohydroxyacid synthase (AHAS) inhibitor herbicides due to the Trp574Leu mutation. An AHAS gene mutation that causes herbicide resistance may have negative pleiotropic effects on plant fitness. This study reports the effects of the Trp574Leu mutation on AHAS activity and reproductive traits of R. sativus. RESULTS: Eight of 17 feral radish accessions presented individuals resistant to metsulfuron‐methyl at 0.5% to >90.0% and all the resistant individuals analyzed showed the Trp574Leu mutation. Without herbicide selection, the AHAS activity was 3.2‐fold higher in the susceptible accession than in the resistant one. The resistant accession was >9000‐fold more resistant to metsulfuron‐methyl and imazethapyr than the susceptible accession. Under low intraspecific competition during two growing seasons, AHAS‐resistant feral radish accessions showed 22–38% and 21–47% lower seed numbers and yield per plant than the susceptible accession. CONCLUSION: This is the first report of fitness cost associated with the AHAS Trp574Leu mutation in R. sativus populations. This fitness cost could reduce frequency of the resistant allele without herbicide selection
EEA Hilario Ascasubi
Fil: Vercellino, Roman B. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pandolfo, Claudio Ezequiel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cantamutto, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Hilario Ascasubi; Argentina
Fil: Presotto, Alejandro Daniel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina - Fuente
- Pest management science 74 (7):1600-1607. (2018 Jul.)
- Materia
-
Raphanus sativus
Actividad Enzimática
Enzyme Activity
Genetics
Mutation
Genética
Mutación - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2180
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AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness costVercellino, Roman BorisPandolfo, Claudio EzequielBreccia, GabrielaCantamutto, Miguel AngelPresotto, Alejandro DanielRaphanus sativusActividad EnzimáticaEnzyme ActivityGeneticsMutationGenéticaMutaciónBACKGROUND: Feral radish (Raphanus sativus L.) is a problematic weed that has become resistant to acetohydroxyacid synthase (AHAS) inhibitor herbicides due to the Trp574Leu mutation. An AHAS gene mutation that causes herbicide resistance may have negative pleiotropic effects on plant fitness. This study reports the effects of the Trp574Leu mutation on AHAS activity and reproductive traits of R. sativus. RESULTS: Eight of 17 feral radish accessions presented individuals resistant to metsulfuron‐methyl at 0.5% to >90.0% and all the resistant individuals analyzed showed the Trp574Leu mutation. Without herbicide selection, the AHAS activity was 3.2‐fold higher in the susceptible accession than in the resistant one. The resistant accession was >9000‐fold more resistant to metsulfuron‐methyl and imazethapyr than the susceptible accession. Under low intraspecific competition during two growing seasons, AHAS‐resistant feral radish accessions showed 22–38% and 21–47% lower seed numbers and yield per plant than the susceptible accession. CONCLUSION: This is the first report of fitness cost associated with the AHAS Trp574Leu mutation in R. sativus populations. This fitness cost could reduce frequency of the resistant allele without herbicide selectionEEA Hilario AscasubiFil: Vercellino, Roman B. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Pandolfo, Claudio Ezequiel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cantamutto, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Hilario Ascasubi; ArgentinaFil: Presotto, Alejandro Daniel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina2018-04-05T15:50:41Z2018-04-05T15:50:41Z2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4849http://hdl.handle.net/20.500.12123/21801526-498X1526-4998https://doi.org/10.1002/ps.4849Pest management science 74 (7):1600-1607. (2018 Jul.)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:17Zoai:localhost:20.500.12123/2180instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:17.399INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
title |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
spellingShingle |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost Vercellino, Roman Boris Raphanus sativus Actividad Enzimática Enzyme Activity Genetics Mutation Genética Mutación |
title_short |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
title_full |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
title_fullStr |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
title_full_unstemmed |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
title_sort |
AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost |
dc.creator.none.fl_str_mv |
Vercellino, Roman Boris Pandolfo, Claudio Ezequiel Breccia, Gabriela Cantamutto, Miguel Angel Presotto, Alejandro Daniel |
author |
Vercellino, Roman Boris |
author_facet |
Vercellino, Roman Boris Pandolfo, Claudio Ezequiel Breccia, Gabriela Cantamutto, Miguel Angel Presotto, Alejandro Daniel |
author_role |
author |
author2 |
Pandolfo, Claudio Ezequiel Breccia, Gabriela Cantamutto, Miguel Angel Presotto, Alejandro Daniel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Raphanus sativus Actividad Enzimática Enzyme Activity Genetics Mutation Genética Mutación |
topic |
Raphanus sativus Actividad Enzimática Enzyme Activity Genetics Mutation Genética Mutación |
dc.description.none.fl_txt_mv |
BACKGROUND: Feral radish (Raphanus sativus L.) is a problematic weed that has become resistant to acetohydroxyacid synthase (AHAS) inhibitor herbicides due to the Trp574Leu mutation. An AHAS gene mutation that causes herbicide resistance may have negative pleiotropic effects on plant fitness. This study reports the effects of the Trp574Leu mutation on AHAS activity and reproductive traits of R. sativus. RESULTS: Eight of 17 feral radish accessions presented individuals resistant to metsulfuron‐methyl at 0.5% to >90.0% and all the resistant individuals analyzed showed the Trp574Leu mutation. Without herbicide selection, the AHAS activity was 3.2‐fold higher in the susceptible accession than in the resistant one. The resistant accession was >9000‐fold more resistant to metsulfuron‐methyl and imazethapyr than the susceptible accession. Under low intraspecific competition during two growing seasons, AHAS‐resistant feral radish accessions showed 22–38% and 21–47% lower seed numbers and yield per plant than the susceptible accession. CONCLUSION: This is the first report of fitness cost associated with the AHAS Trp574Leu mutation in R. sativus populations. This fitness cost could reduce frequency of the resistant allele without herbicide selection EEA Hilario Ascasubi Fil: Vercellino, Roman B. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Pandolfo, Claudio Ezequiel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cantamutto, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Hilario Ascasubi; Argentina Fil: Presotto, Alejandro Daniel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina |
description |
BACKGROUND: Feral radish (Raphanus sativus L.) is a problematic weed that has become resistant to acetohydroxyacid synthase (AHAS) inhibitor herbicides due to the Trp574Leu mutation. An AHAS gene mutation that causes herbicide resistance may have negative pleiotropic effects on plant fitness. This study reports the effects of the Trp574Leu mutation on AHAS activity and reproductive traits of R. sativus. RESULTS: Eight of 17 feral radish accessions presented individuals resistant to metsulfuron‐methyl at 0.5% to >90.0% and all the resistant individuals analyzed showed the Trp574Leu mutation. Without herbicide selection, the AHAS activity was 3.2‐fold higher in the susceptible accession than in the resistant one. The resistant accession was >9000‐fold more resistant to metsulfuron‐methyl and imazethapyr than the susceptible accession. Under low intraspecific competition during two growing seasons, AHAS‐resistant feral radish accessions showed 22–38% and 21–47% lower seed numbers and yield per plant than the susceptible accession. CONCLUSION: This is the first report of fitness cost associated with the AHAS Trp574Leu mutation in R. sativus populations. This fitness cost could reduce frequency of the resistant allele without herbicide selection |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-05T15:50:41Z 2018-04-05T15:50:41Z 2018-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4849 http://hdl.handle.net/20.500.12123/2180 1526-498X 1526-4998 https://doi.org/10.1002/ps.4849 |
url |
https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4849 http://hdl.handle.net/20.500.12123/2180 https://doi.org/10.1002/ps.4849 |
identifier_str_mv |
1526-498X 1526-4998 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Pest management science 74 (7):1600-1607. (2018 Jul.) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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12.559606 |