A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents

Autores
Rodriguez, Paula; Magallanes Noguera, Cynthia Alejandra; Menendez, Pilar; Orden, Alejandro Agustin; González, David; Kurina Sanz, Marcela Beatriz; Rodriguez, Sonia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The reported anti-Prelog selectivity in Raphanus sativus hairy roots prompted us to search for similar activity in other R. sativus systems, such as in vitro germinated sprouts, edible roots, and its associated endophytic microorganisms. Two test substrates were used in these studies: acetophenone and rac-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strains were evaluated for their ability to reduce both substrates. Among the isolates, two bacteria and a fungus having the potential to be used as biocatalysts were found: Bacillus megaterium which reduces acetophenone with excellent anti-Prelog selectivity, and Pseudomonas sp. and Penicillium chrysogenum with enantioselectivity and stereoselectivity for the reduction of rac-ethyl 2-ethyl-3-oxobutanoate, respectively.
Fil: Rodriguez, Paula. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Fil: Magallanes Noguera, Cynthia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Menendez, Pilar. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Fil: Orden, Alejandro Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: González, David. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Fil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Rodriguez, Sonia. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Materia
Raphanus Sativus
Bio-Reduction
Sec-Alcohols
Endophytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5851
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5851
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agentsRodriguez, PaulaMagallanes Noguera, Cynthia AlejandraMenendez, PilarOrden, Alejandro AgustinGonzález, DavidKurina Sanz, Marcela BeatrizRodriguez, SoniaRaphanus SativusBio-ReductionSec-AlcoholsEndophyteshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The reported anti-Prelog selectivity in Raphanus sativus hairy roots prompted us to search for similar activity in other R. sativus systems, such as in vitro germinated sprouts, edible roots, and its associated endophytic microorganisms. Two test substrates were used in these studies: acetophenone and rac-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strains were evaluated for their ability to reduce both substrates. Among the isolates, two bacteria and a fungus having the potential to be used as biocatalysts were found: Bacillus megaterium which reduces acetophenone with excellent anti-Prelog selectivity, and Pseudomonas sp. and Penicillium chrysogenum with enantioselectivity and stereoselectivity for the reduction of rac-ethyl 2-ethyl-3-oxobutanoate, respectively.Fil: Rodriguez, Paula. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; UruguayFil: Magallanes Noguera, Cynthia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Menendez, Pilar. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; UruguayFil: Orden, Alejandro Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: González, David. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; UruguayFil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Rodriguez, Sonia. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; UruguayTaylor & Francis2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5851Rodriguez, Paula; Magallanes Noguera, Cynthia Alejandra; Menendez, Pilar; Orden, Alejandro Agustin; González, David; et al.; A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents; Taylor & Francis; Biocatalysis and Biotransformation; 33; 2; 1-2015; 121-1291024-2422enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/10242422.2015.1053471info:eu-repo/semantics/altIdentifier/doi/10.3109/10242422.2015.1053471info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:20Zoai:ri.conicet.gov.ar:11336/5851instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:20.724CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
title A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
spellingShingle A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
Rodriguez, Paula
Raphanus Sativus
Bio-Reduction
Sec-Alcohols
Endophytes
title_short A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
title_full A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
title_fullStr A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
title_full_unstemmed A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
title_sort A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
dc.creator.none.fl_str_mv Rodriguez, Paula
Magallanes Noguera, Cynthia Alejandra
Menendez, Pilar
Orden, Alejandro Agustin
González, David
Kurina Sanz, Marcela Beatriz
Rodriguez, Sonia
author Rodriguez, Paula
author_facet Rodriguez, Paula
Magallanes Noguera, Cynthia Alejandra
Menendez, Pilar
Orden, Alejandro Agustin
González, David
Kurina Sanz, Marcela Beatriz
Rodriguez, Sonia
author_role author
author2 Magallanes Noguera, Cynthia Alejandra
Menendez, Pilar
Orden, Alejandro Agustin
González, David
Kurina Sanz, Marcela Beatriz
Rodriguez, Sonia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Raphanus Sativus
Bio-Reduction
Sec-Alcohols
Endophytes
topic Raphanus Sativus
Bio-Reduction
Sec-Alcohols
Endophytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The reported anti-Prelog selectivity in Raphanus sativus hairy roots prompted us to search for similar activity in other R. sativus systems, such as in vitro germinated sprouts, edible roots, and its associated endophytic microorganisms. Two test substrates were used in these studies: acetophenone and rac-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strains were evaluated for their ability to reduce both substrates. Among the isolates, two bacteria and a fungus having the potential to be used as biocatalysts were found: Bacillus megaterium which reduces acetophenone with excellent anti-Prelog selectivity, and Pseudomonas sp. and Penicillium chrysogenum with enantioselectivity and stereoselectivity for the reduction of rac-ethyl 2-ethyl-3-oxobutanoate, respectively.
Fil: Rodriguez, Paula. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Fil: Magallanes Noguera, Cynthia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Menendez, Pilar. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Fil: Orden, Alejandro Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: González, David. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
Fil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Rodriguez, Sonia. Universidad de la República. Facultad de Química. Departamento de Biociencias y Departamento de Química Orgánica. Laboratorio de Biocatálisis y Biotransformaciones; Uruguay
description The reported anti-Prelog selectivity in Raphanus sativus hairy roots prompted us to search for similar activity in other R. sativus systems, such as in vitro germinated sprouts, edible roots, and its associated endophytic microorganisms. Two test substrates were used in these studies: acetophenone and rac-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strains were evaluated for their ability to reduce both substrates. Among the isolates, two bacteria and a fungus having the potential to be used as biocatalysts were found: Bacillus megaterium which reduces acetophenone with excellent anti-Prelog selectivity, and Pseudomonas sp. and Penicillium chrysogenum with enantioselectivity and stereoselectivity for the reduction of rac-ethyl 2-ethyl-3-oxobutanoate, respectively.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5851
Rodriguez, Paula; Magallanes Noguera, Cynthia Alejandra; Menendez, Pilar; Orden, Alejandro Agustin; González, David; et al.; A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents; Taylor & Francis; Biocatalysis and Biotransformation; 33; 2; 1-2015; 121-129
1024-2422
url http://hdl.handle.net/11336/5851
identifier_str_mv Rodriguez, Paula; Magallanes Noguera, Cynthia Alejandra; Menendez, Pilar; Orden, Alejandro Agustin; González, David; et al.; A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents; Taylor & Francis; Biocatalysis and Biotransformation; 33; 2; 1-2015; 121-129
1024-2422
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/10242422.2015.1053471
info:eu-repo/semantics/altIdentifier/doi/10.3109/10242422.2015.1053471
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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