Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters
- Autores
- Simeone, Melina; Gomez, Claudio Andres; Bertalmío, Ana; Ruiz, Esperanza; Hauteville, Claudia; Godoy Suarez, Laura Magdalena; Tito, Blas Alfredo; García, María Laura
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.
EEA Concordia
Fil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bertalmío, Ana. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental Salto Grande; Uruguay
Fil: Ruiz, Esperanza. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina
Fil: Hauteville, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Godoy Suarez, Laura Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Tito, Blas Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina - Fuente
- Plant Pathology (First published: 18 January 2021)
- Materia
-
Citrus
Enfermedades de las Plantas
Diagnóstico
PCR
Virus de las Plantas
Plant Diseases
Diagnosis
Plant Viruses
Virus de la Psoriasis de los Cítricos
Citrus Psorosis Virus - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8837
Ver los metadatos del registro completo
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Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parametersSimeone, MelinaGomez, Claudio AndresBertalmío, AnaRuiz, EsperanzaHauteville, ClaudiaGodoy Suarez, Laura MagdalenaTito, Blas AlfredoGarcía, María LauraCitrusEnfermedades de las PlantasDiagnósticoPCRVirus de las PlantasPlant DiseasesDiagnosisPlant VirusesVirus de la Psoriasis de los CítricosCitrus Psorosis VirusCitrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.EEA ConcordiaFil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Bertalmío, Ana. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental Salto Grande; UruguayFil: Ruiz, Esperanza. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; ArgentinaFil: Hauteville, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Godoy Suarez, Laura Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Tito, Blas Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley2021-03-08T16:24:01Z2021-03-08T16:24:01Z2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8837https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.133411365-3059https://doi.org/10.1111/ppa.13341Plant Pathology (First published: 18 January 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/ERIOS-630081/AR./Incremento en la calidad, cantidad y diversidad de la producción frutícola de Entre Ríosinfo:eu-repograntAgreement/INTA/2019-PD-E4-I081-001/2019-PD-E4-I081-001/AR./Generación de reactivos, desarrollo de metodologías, validación y acreditación de ensayos para el diagnóstico de patógenos vegetales.info:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001/2019-RIST-E4-I091-001/AR./Multiplicación de propágulos de sanidad controladainfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:09Zoai:localhost:20.500.12123/8837instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:09.473INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| spellingShingle |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters Simeone, Melina Citrus Enfermedades de las Plantas Diagnóstico PCR Virus de las Plantas Plant Diseases Diagnosis Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus |
| title_short |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_full |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_fullStr |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_full_unstemmed |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_sort |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| dc.creator.none.fl_str_mv |
Simeone, Melina Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura |
| author |
Simeone, Melina |
| author_facet |
Simeone, Melina Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura |
| author_role |
author |
| author2 |
Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Citrus Enfermedades de las Plantas Diagnóstico PCR Virus de las Plantas Plant Diseases Diagnosis Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus |
| topic |
Citrus Enfermedades de las Plantas Diagnóstico PCR Virus de las Plantas Plant Diseases Diagnosis Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus |
| dc.description.none.fl_txt_mv |
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees. EEA Concordia Fil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bertalmío, Ana. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental Salto Grande; Uruguay Fil: Ruiz, Esperanza. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina Fil: Hauteville, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Godoy Suarez, Laura Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Tito, Blas Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina |
| description |
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees. |
| publishDate |
2021 |
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2021-03-08T16:24:01Z 2021-03-08T16:24:01Z 2021-01 |
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eng |
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