Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach

Autores
Oberschelp, Gustavo Pedro Javier; Guarnaschelli, Ana Beatriz; Teson, Natalia; Harrand, Leonel; Podestá, Florencio Esteban; Margarit, Ezequiel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The ability of plants to cope with frost events relies on the physiological and molecular responses triggered by cold temperatures. This process, named acclimation, involves reprogramming gene expression in order to adjust metabolism. Planted Eucalyptus species are highly productive but most of them are frost sensitive. However, acclimation process varies among species and environmental conditions, promoting more or less frost damage in young plantations of frost-prone areas. To identify metabolites and proteins responsible for these differences, two acclimation regimes were imposed to seedling of Eucalyptus grandis Hill ex Maiden (Eg), Eucalyptus dunnii Maiden (Ed) and Eucalyptus benthamii Maiden Cambage (Eb), and leaves submitted to biochemical and molecular analyses. Further, seedlings were used for simulated frosts in order to test the acclimation status effect on frost tolerance. Eb showed higher frost tolerance than Ed and Eg under control and acclimation scenarios, possibly due to its higher accumulation of phenolics, anthocyanins and soluble sugars as well as lower levels of photosynthetic pigments and related proteins. Also, a rise in frost tolerance and in osmoprotectants and antioxidants was observed for all the species due to cold acclimation treatment. Interestingly, metabolic profiles differed among species, suggesting different mechanisms to endure frosts and, probably, different requirements for cold acclimation. Shotgun proteomics reinforced differences and commonalities and supported metabolome observations. An in depth understanding of these responses could help to safeguard planted forests productivity through breeding of tolerant genetic material.
EEA Concordia
Fil: Oberschelp, Gustavo Pedro Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Guarnaschelli, Ana Beatriz. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Tesón, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina.
Fil: Harrand, Leonel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fil: Margarit, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fuente
Plant Physiology and Biochemistry (Available online 26 May 2020)
Materia
Eucalyptus
Eucalyptus grandis
Eucalyptus benthamii
Variedades
Aclimatación
Tolerancia al Frío
Varieties
Acclimatization
Cold Tolerance
Eucalyptus dunnii
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7381
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7381
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spelling Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approachOberschelp, Gustavo Pedro JavierGuarnaschelli, Ana BeatrizTeson, NataliaHarrand, LeonelPodestá, Florencio EstebanMargarit, EzequielEucalyptusEucalyptus grandisEucalyptus benthamiiVariedadesAclimataciónTolerancia al FríoVarietiesAcclimatizationCold ToleranceEucalyptus dunniiThe ability of plants to cope with frost events relies on the physiological and molecular responses triggered by cold temperatures. This process, named acclimation, involves reprogramming gene expression in order to adjust metabolism. Planted Eucalyptus species are highly productive but most of them are frost sensitive. However, acclimation process varies among species and environmental conditions, promoting more or less frost damage in young plantations of frost-prone areas. To identify metabolites and proteins responsible for these differences, two acclimation regimes were imposed to seedling of Eucalyptus grandis Hill ex Maiden (Eg), Eucalyptus dunnii Maiden (Ed) and Eucalyptus benthamii Maiden Cambage (Eb), and leaves submitted to biochemical and molecular analyses. Further, seedlings were used for simulated frosts in order to test the acclimation status effect on frost tolerance. Eb showed higher frost tolerance than Ed and Eg under control and acclimation scenarios, possibly due to its higher accumulation of phenolics, anthocyanins and soluble sugars as well as lower levels of photosynthetic pigments and related proteins. Also, a rise in frost tolerance and in osmoprotectants and antioxidants was observed for all the species due to cold acclimation treatment. Interestingly, metabolic profiles differed among species, suggesting different mechanisms to endure frosts and, probably, different requirements for cold acclimation. Shotgun proteomics reinforced differences and commonalities and supported metabolome observations. An in depth understanding of these responses could help to safeguard planted forests productivity through breeding of tolerant genetic material.EEA ConcordiaFil: Oberschelp, Gustavo Pedro Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Guarnaschelli, Ana Beatriz. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Tesón, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina.Fil: Harrand, Leonel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; ArgentinaFil: Margarit, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2022-06-092020-06-09T14:12:23Z2020-06-09T14:12:23Z2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7381https://www.sciencedirect.com/science/article/abs/pii/S09819428203025880981-94281873-2690https://doi.org/10.1016/j.plaphy.2020.05.026Plant Physiology and Biochemistry (Available online 26 May 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:57Zoai:localhost:20.500.12123/7381instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:57.792INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
title Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
spellingShingle Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
Oberschelp, Gustavo Pedro Javier
Eucalyptus
Eucalyptus grandis
Eucalyptus benthamii
Variedades
Aclimatación
Tolerancia al Frío
Varieties
Acclimatization
Cold Tolerance
Eucalyptus dunnii
title_short Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
title_full Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
title_fullStr Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
title_full_unstemmed Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
title_sort Cold acclimation and freezing tolerance in three Eucalyptus species: A metabolomic and proteomic approach
dc.creator.none.fl_str_mv Oberschelp, Gustavo Pedro Javier
Guarnaschelli, Ana Beatriz
Teson, Natalia
Harrand, Leonel
Podestá, Florencio Esteban
Margarit, Ezequiel
author Oberschelp, Gustavo Pedro Javier
author_facet Oberschelp, Gustavo Pedro Javier
Guarnaschelli, Ana Beatriz
Teson, Natalia
Harrand, Leonel
Podestá, Florencio Esteban
Margarit, Ezequiel
author_role author
author2 Guarnaschelli, Ana Beatriz
Teson, Natalia
Harrand, Leonel
Podestá, Florencio Esteban
Margarit, Ezequiel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Eucalyptus
Eucalyptus grandis
Eucalyptus benthamii
Variedades
Aclimatación
Tolerancia al Frío
Varieties
Acclimatization
Cold Tolerance
Eucalyptus dunnii
topic Eucalyptus
Eucalyptus grandis
Eucalyptus benthamii
Variedades
Aclimatación
Tolerancia al Frío
Varieties
Acclimatization
Cold Tolerance
Eucalyptus dunnii
dc.description.none.fl_txt_mv The ability of plants to cope with frost events relies on the physiological and molecular responses triggered by cold temperatures. This process, named acclimation, involves reprogramming gene expression in order to adjust metabolism. Planted Eucalyptus species are highly productive but most of them are frost sensitive. However, acclimation process varies among species and environmental conditions, promoting more or less frost damage in young plantations of frost-prone areas. To identify metabolites and proteins responsible for these differences, two acclimation regimes were imposed to seedling of Eucalyptus grandis Hill ex Maiden (Eg), Eucalyptus dunnii Maiden (Ed) and Eucalyptus benthamii Maiden Cambage (Eb), and leaves submitted to biochemical and molecular analyses. Further, seedlings were used for simulated frosts in order to test the acclimation status effect on frost tolerance. Eb showed higher frost tolerance than Ed and Eg under control and acclimation scenarios, possibly due to its higher accumulation of phenolics, anthocyanins and soluble sugars as well as lower levels of photosynthetic pigments and related proteins. Also, a rise in frost tolerance and in osmoprotectants and antioxidants was observed for all the species due to cold acclimation treatment. Interestingly, metabolic profiles differed among species, suggesting different mechanisms to endure frosts and, probably, different requirements for cold acclimation. Shotgun proteomics reinforced differences and commonalities and supported metabolome observations. An in depth understanding of these responses could help to safeguard planted forests productivity through breeding of tolerant genetic material.
EEA Concordia
Fil: Oberschelp, Gustavo Pedro Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Guarnaschelli, Ana Beatriz. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Tesón, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina.
Fil: Harrand, Leonel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
Fil: Margarit, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosinteticos y Bioquímicos; Argentina
description The ability of plants to cope with frost events relies on the physiological and molecular responses triggered by cold temperatures. This process, named acclimation, involves reprogramming gene expression in order to adjust metabolism. Planted Eucalyptus species are highly productive but most of them are frost sensitive. However, acclimation process varies among species and environmental conditions, promoting more or less frost damage in young plantations of frost-prone areas. To identify metabolites and proteins responsible for these differences, two acclimation regimes were imposed to seedling of Eucalyptus grandis Hill ex Maiden (Eg), Eucalyptus dunnii Maiden (Ed) and Eucalyptus benthamii Maiden Cambage (Eb), and leaves submitted to biochemical and molecular analyses. Further, seedlings were used for simulated frosts in order to test the acclimation status effect on frost tolerance. Eb showed higher frost tolerance than Ed and Eg under control and acclimation scenarios, possibly due to its higher accumulation of phenolics, anthocyanins and soluble sugars as well as lower levels of photosynthetic pigments and related proteins. Also, a rise in frost tolerance and in osmoprotectants and antioxidants was observed for all the species due to cold acclimation treatment. Interestingly, metabolic profiles differed among species, suggesting different mechanisms to endure frosts and, probably, different requirements for cold acclimation. Shotgun proteomics reinforced differences and commonalities and supported metabolome observations. An in depth understanding of these responses could help to safeguard planted forests productivity through breeding of tolerant genetic material.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-09T14:12:23Z
2020-06-09T14:12:23Z
2020-05
info:eu-repo/date/embargoEnd/2022-06-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7381
https://www.sciencedirect.com/science/article/abs/pii/S0981942820302588
0981-9428
1873-2690
https://doi.org/10.1016/j.plaphy.2020.05.026
url http://hdl.handle.net/20.500.12123/7381
https://www.sciencedirect.com/science/article/abs/pii/S0981942820302588
https://doi.org/10.1016/j.plaphy.2020.05.026
identifier_str_mv 0981-9428
1873-2690
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Plant Physiology and Biochemistry (Available online 26 May 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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