Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages

Autores
Blanco, Andrés; Pignata, María L.; Lascano, Hernan Ramiro; Rodriguez, Judith Hebelen
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Blanco, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; Argentina
Fil: Blanco, Andrés. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Pignata, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; Argentina
Fil: Pignata, María L. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina
Fil: Rodriguez, Judith H. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores. Argentina
Fil: Rodriguez, Judith H. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fuente
Environmental Science and Pollution Research 28: 22843–22852. (2021)
Materia
Soybeans
Toxicity
Abiotic Stress
Bioaccumulation
Hydroponics
Toxicidad
Estrés Abiótico
Glycine Max
Soja
Bioacumulación
Cultivo Hidropónico
Pb
Morpho-Physiological Parameters
Hydroponic System
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10333
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network_name_str INTA Digital (INTA)
spelling Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stagesBlanco, AndrésPignata, María L.Lascano, Hernan RamiroRodriguez, Judith HebelenSoybeansToxicityAbiotic StressBioaccumulationHydroponicsToxicidadEstrés AbióticoGlycine MaxSojaBioacumulaciónCultivo HidropónicoPbMorpho-Physiological ParametersHydroponic SystemLead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Blanco, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; ArgentinaFil: Blanco, Andrés. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Pignata, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; ArgentinaFil: Pignata, María L. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. ArgentinaFil: Rodriguez, Judith H. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores. ArgentinaFil: Rodriguez, Judith H. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaSpringer2021-09-22T18:56:21Z2021-09-22T18:56:21Z2021-01-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10333https://link.springer.com/article/10.1007/s11356-021-12389-90944-13441614-7499 (online)https://doi.org/10.1007/s11356-021-12389-9Environmental Science and Pollution Research 28: 22843–22852. (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-06T09:40:59Zoai:localhost:20.500.12123/10333instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:40:59.753INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
title Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
spellingShingle Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
Blanco, Andrés
Soybeans
Toxicity
Abiotic Stress
Bioaccumulation
Hydroponics
Toxicidad
Estrés Abiótico
Glycine Max
Soja
Bioacumulación
Cultivo Hidropónico
Pb
Morpho-Physiological Parameters
Hydroponic System
title_short Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
title_full Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
title_fullStr Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
title_full_unstemmed Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
title_sort Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages
dc.creator.none.fl_str_mv Blanco, Andrés
Pignata, María L.
Lascano, Hernan Ramiro
Rodriguez, Judith Hebelen
author Blanco, Andrés
author_facet Blanco, Andrés
Pignata, María L.
Lascano, Hernan Ramiro
Rodriguez, Judith Hebelen
author_role author
author2 Pignata, María L.
Lascano, Hernan Ramiro
Rodriguez, Judith Hebelen
author2_role author
author
author
dc.subject.none.fl_str_mv Soybeans
Toxicity
Abiotic Stress
Bioaccumulation
Hydroponics
Toxicidad
Estrés Abiótico
Glycine Max
Soja
Bioacumulación
Cultivo Hidropónico
Pb
Morpho-Physiological Parameters
Hydroponic System
topic Soybeans
Toxicity
Abiotic Stress
Bioaccumulation
Hydroponics
Toxicidad
Estrés Abiótico
Glycine Max
Soja
Bioacumulación
Cultivo Hidropónico
Pb
Morpho-Physiological Parameters
Hydroponic System
dc.description.none.fl_txt_mv Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Blanco, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; Argentina
Fil: Blanco, Andrés. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Pignata, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores; Argentina
Fil: Pignata, María L. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina
Fil: Rodriguez, Judith H. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal (IMBIV). Área Contaminación y Bioindicadores. Argentina
Fil: Rodriguez, Judith H. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
description Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-22T18:56:21Z
2021-09-22T18:56:21Z
2021-01-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10333
https://link.springer.com/article/10.1007/s11356-021-12389-9
0944-1344
1614-7499 (online)
https://doi.org/10.1007/s11356-021-12389-9
url http://hdl.handle.net/20.500.12123/10333
https://link.springer.com/article/10.1007/s11356-021-12389-9
https://doi.org/10.1007/s11356-021-12389-9
identifier_str_mv 0944-1344
1614-7499 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Environmental Science and Pollution Research 28: 22843–22852. (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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