Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses

Autores
Di Martino, Ana Maria; Truchet, Daniela María; Butti, Mariano; Franco, María Del Rocío; Mendieta, Julieta Renée; Menone, Mirta Luján; Pérez, Débora Jesabel
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Lead (Pb) contamination in aquatic environments is increasing worldwide, yet studies integrating metal uptake, plant tolerance mechanisms, and post-remediation biomass valorization remain limited. Bridging phytoremediation efficiency with sustainable biomass management is essential to advance nature-based solutions. Objectives: This study evaluated the phytoremediation potential of the emergent macrophyte Typha latifolia, integrating Pb uptake and removal efficiency, physiological stress responses, and biogas production from Pb-exposed biomass. Methods: Plants were exposed under controlled hydroponic conditions to Pb concentrations of 50, 100, 1,000, and 10,000 µg L⁻¹ for 48 and 168 hours. Pb accumulation and translocation were quantified in roots and leaves, along with changes in aqueous Pb concentrations. Morphological traits and oxidative stress biomarkers were assessed in roots and leaves, while photosynthetic pigments were measured in leaves. Multivariate analyses, including the integrated biomarker response (IBR) index and principal component analysis (PCA), were applied. Biogas production assays were conducted using biomass from control plants and selected Pb treatments (1,000 and 10,000 µg L⁻¹). Results: Lead accumulated mainly in roots, with limited translocation to leaves. Aqueous Pb concentrations decreased by more than 60% at the highest exposure after 168 h. Despite substantial Pb accumulation, plants showed minimal morphological changes, indicating high short-term tolerance. Biochemical responses suggested activation of antioxidant defenses, together with a moderate increase in chlorophyll content in leaves at higher Pb concentrations, indicative of a physiological stress response supported by IBR and PCA analyses. Notably, shoots exposed to 1,000 µg L⁻¹ Pb produced the highest biogas yield. Conclusions: T. latifolia exhibits strong Pb tolerance, effective phytostabilization capacity, and maintains biomass suitability for energy recovery after exposure. These findings highlight its dual role in Pb-contaminated systems, combining remediation potential with sustainable biomass valorization within a circular bioeconomy framework.
EEA Pergamino
Fil: Di Martino, Ana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; Argentina
Fil: Truchet, Daniela M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina
Fil: Butti, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelos y Agua; Argentina
Fil: Franco, María R. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Mendieta, Julieta R. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; Argentina
Fil: Mendieta, Julieta R. Universidad Nacional de Mar del Plata; Argentina
Fil: Menone, Mirta L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina
Fil: Pérez, Débora J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fuente
Journal of Trace Elements and Minerals 16 : 100297. (June 2026)
Materia
Biogás
Macrofito
Biomarcadores
Economía Circular
Biogas
Macrophytes
Biomarkers
Circular Economy
Phytoremediation
Typha
Fitodecontaminación
Typha latifolia
Translocation
Phytoremediation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25859
Acceder
id INTADig_a875c4e54e2043b22e39d622a67247c8
oai_identifier_str oai:localhost:20.500.12123/25859
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responsesDi Martino, Ana MariaTruchet, Daniela MaríaButti, MarianoFranco, María Del RocíoMendieta, Julieta RenéeMenone, Mirta LujánPérez, Débora JesabelBiogásMacrofitoBiomarcadoresEconomía CircularBiogasMacrophytesBiomarkersCircular EconomyPhytoremediationTyphaFitodecontaminaciónTypha latifoliaTranslocationPhytoremediationBackground: Lead (Pb) contamination in aquatic environments is increasing worldwide, yet studies integrating metal uptake, plant tolerance mechanisms, and post-remediation biomass valorization remain limited. Bridging phytoremediation efficiency with sustainable biomass management is essential to advance nature-based solutions. Objectives: This study evaluated the phytoremediation potential of the emergent macrophyte Typha latifolia, integrating Pb uptake and removal efficiency, physiological stress responses, and biogas production from Pb-exposed biomass. Methods: Plants were exposed under controlled hydroponic conditions to Pb concentrations of 50, 100, 1,000, and 10,000 µg L⁻¹ for 48 and 168 hours. Pb accumulation and translocation were quantified in roots and leaves, along with changes in aqueous Pb concentrations. Morphological traits and oxidative stress biomarkers were assessed in roots and leaves, while photosynthetic pigments were measured in leaves. Multivariate analyses, including the integrated biomarker response (IBR) index and principal component analysis (PCA), were applied. Biogas production assays were conducted using biomass from control plants and selected Pb treatments (1,000 and 10,000 µg L⁻¹). Results: Lead accumulated mainly in roots, with limited translocation to leaves. Aqueous Pb concentrations decreased by more than 60% at the highest exposure after 168 h. Despite substantial Pb accumulation, plants showed minimal morphological changes, indicating high short-term tolerance. Biochemical responses suggested activation of antioxidant defenses, together with a moderate increase in chlorophyll content in leaves at higher Pb concentrations, indicative of a physiological stress response supported by IBR and PCA analyses. Notably, shoots exposed to 1,000 µg L⁻¹ Pb produced the highest biogas yield. Conclusions: T. latifolia exhibits strong Pb tolerance, effective phytostabilization capacity, and maintains biomass suitability for energy recovery after exposure. These findings highlight its dual role in Pb-contaminated systems, combining remediation potential with sustainable biomass valorization within a circular bioeconomy framework.EEA PergaminoFil: Di Martino, Ana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; ArgentinaFil: Truchet, Daniela M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; ArgentinaFil: Butti, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelos y Agua; ArgentinaFil: Franco, María R. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Mendieta, Julieta R. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; ArgentinaFil: Mendieta, Julieta R. Universidad Nacional de Mar del Plata; ArgentinaFil: Menone, Mirta L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; ArgentinaFil: Pérez, Débora J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaElsevier2026-04-20T10:06:12Z2026-04-20T10:06:12Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25859https://www.sciencedirect.com/science/article/pii/S27730506260002002773-0506 (online)https://doi.org/10.1016/j.jtemin.2026.100297Journal of Trace Elements and Minerals 16 : 100297. (June 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediacióninfo:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregadoinfo:eu-repograntAgreement/INTA/2023-PE-L02-I010, Mitigación de externalidades de los sistemas productivos Pampeanosinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-23T10:40:36Zoai:localhost:20.500.12123/25859instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-23 10:40:36.453INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
title Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
spellingShingle Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
Di Martino, Ana Maria
Biogás
Macrofito
Biomarcadores
Economía Circular
Biogas
Macrophytes
Biomarkers
Circular Economy
Phytoremediation
Typha
Fitodecontaminación
Typha latifolia
Translocation
Phytoremediation
title_short Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
title_full Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
title_fullStr Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
title_full_unstemmed Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
title_sort Lead phytoremediation and selective biogas production from Typha latifolia L. : physiological and biochemical responses
dc.creator.none.fl_str_mv Di Martino, Ana Maria
Truchet, Daniela María
Butti, Mariano
Franco, María Del Rocío
Mendieta, Julieta Renée
Menone, Mirta Luján
Pérez, Débora Jesabel
author Di Martino, Ana Maria
author_facet Di Martino, Ana Maria
Truchet, Daniela María
Butti, Mariano
Franco, María Del Rocío
Mendieta, Julieta Renée
Menone, Mirta Luján
Pérez, Débora Jesabel
author_role author
author2 Truchet, Daniela María
Butti, Mariano
Franco, María Del Rocío
Mendieta, Julieta Renée
Menone, Mirta Luján
Pérez, Débora Jesabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biogás
Macrofito
Biomarcadores
Economía Circular
Biogas
Macrophytes
Biomarkers
Circular Economy
Phytoremediation
Typha
Fitodecontaminación
Typha latifolia
Translocation
Phytoremediation
topic Biogás
Macrofito
Biomarcadores
Economía Circular
Biogas
Macrophytes
Biomarkers
Circular Economy
Phytoremediation
Typha
Fitodecontaminación
Typha latifolia
Translocation
Phytoremediation
dc.description.none.fl_txt_mv Background: Lead (Pb) contamination in aquatic environments is increasing worldwide, yet studies integrating metal uptake, plant tolerance mechanisms, and post-remediation biomass valorization remain limited. Bridging phytoremediation efficiency with sustainable biomass management is essential to advance nature-based solutions. Objectives: This study evaluated the phytoremediation potential of the emergent macrophyte Typha latifolia, integrating Pb uptake and removal efficiency, physiological stress responses, and biogas production from Pb-exposed biomass. Methods: Plants were exposed under controlled hydroponic conditions to Pb concentrations of 50, 100, 1,000, and 10,000 µg L⁻¹ for 48 and 168 hours. Pb accumulation and translocation were quantified in roots and leaves, along with changes in aqueous Pb concentrations. Morphological traits and oxidative stress biomarkers were assessed in roots and leaves, while photosynthetic pigments were measured in leaves. Multivariate analyses, including the integrated biomarker response (IBR) index and principal component analysis (PCA), were applied. Biogas production assays were conducted using biomass from control plants and selected Pb treatments (1,000 and 10,000 µg L⁻¹). Results: Lead accumulated mainly in roots, with limited translocation to leaves. Aqueous Pb concentrations decreased by more than 60% at the highest exposure after 168 h. Despite substantial Pb accumulation, plants showed minimal morphological changes, indicating high short-term tolerance. Biochemical responses suggested activation of antioxidant defenses, together with a moderate increase in chlorophyll content in leaves at higher Pb concentrations, indicative of a physiological stress response supported by IBR and PCA analyses. Notably, shoots exposed to 1,000 µg L⁻¹ Pb produced the highest biogas yield. Conclusions: T. latifolia exhibits strong Pb tolerance, effective phytostabilization capacity, and maintains biomass suitability for energy recovery after exposure. These findings highlight its dual role in Pb-contaminated systems, combining remediation potential with sustainable biomass valorization within a circular bioeconomy framework.
EEA Pergamino
Fil: Di Martino, Ana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; Argentina
Fil: Truchet, Daniela M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina
Fil: Butti, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelos y Agua; Argentina
Fil: Franco, María R. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Mendieta, Julieta R. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; Argentina
Fil: Mendieta, Julieta R. Universidad Nacional de Mar del Plata; Argentina
Fil: Menone, Mirta L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología; Argentina
Fil: Pérez, Débora J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
description Background: Lead (Pb) contamination in aquatic environments is increasing worldwide, yet studies integrating metal uptake, plant tolerance mechanisms, and post-remediation biomass valorization remain limited. Bridging phytoremediation efficiency with sustainable biomass management is essential to advance nature-based solutions. Objectives: This study evaluated the phytoremediation potential of the emergent macrophyte Typha latifolia, integrating Pb uptake and removal efficiency, physiological stress responses, and biogas production from Pb-exposed biomass. Methods: Plants were exposed under controlled hydroponic conditions to Pb concentrations of 50, 100, 1,000, and 10,000 µg L⁻¹ for 48 and 168 hours. Pb accumulation and translocation were quantified in roots and leaves, along with changes in aqueous Pb concentrations. Morphological traits and oxidative stress biomarkers were assessed in roots and leaves, while photosynthetic pigments were measured in leaves. Multivariate analyses, including the integrated biomarker response (IBR) index and principal component analysis (PCA), were applied. Biogas production assays were conducted using biomass from control plants and selected Pb treatments (1,000 and 10,000 µg L⁻¹). Results: Lead accumulated mainly in roots, with limited translocation to leaves. Aqueous Pb concentrations decreased by more than 60% at the highest exposure after 168 h. Despite substantial Pb accumulation, plants showed minimal morphological changes, indicating high short-term tolerance. Biochemical responses suggested activation of antioxidant defenses, together with a moderate increase in chlorophyll content in leaves at higher Pb concentrations, indicative of a physiological stress response supported by IBR and PCA analyses. Notably, shoots exposed to 1,000 µg L⁻¹ Pb produced the highest biogas yield. Conclusions: T. latifolia exhibits strong Pb tolerance, effective phytostabilization capacity, and maintains biomass suitability for energy recovery after exposure. These findings highlight its dual role in Pb-contaminated systems, combining remediation potential with sustainable biomass valorization within a circular bioeconomy framework.
publishDate 2026
dc.date.none.fl_str_mv 2026-04-20T10:06:12Z
2026-04-20T10:06:12Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25859
https://www.sciencedirect.com/science/article/pii/S2773050626000200
2773-0506 (online)
https://doi.org/10.1016/j.jtemin.2026.100297
url http://hdl.handle.net/20.500.12123/25859
https://www.sciencedirect.com/science/article/pii/S2773050626000200
https://doi.org/10.1016/j.jtemin.2026.100297
identifier_str_mv 2773-0506 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediación
info:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregado
info:eu-repograntAgreement/INTA/2023-PE-L02-I010, Mitigación de externalidades de los sistemas productivos Pampeanos
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Trace Elements and Minerals 16 : 100297. (June 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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