Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows
- Autores
- Camussone, Cecilia; Reidel, Ivana Gabriela; Molineri, Ana Ines; Cicotello, Joaquin; Miotti, Camila; Suarez Archilla, Guillermo; Curti, C.C.; Veaute, Carolina Melania Isabel; Calvinho, Luis Fernando
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation.
EEA Rafaela
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina
Fil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.
Fil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cicotello, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Cicotello, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curti, C.C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Research in Veterinary Science 145 : 177-187 (July 2022)
- Materia
-
Novilla
Vaca
Staphylococcus aureus
Inmunización
Vacuna
Proteínas Recombinantes
Heifers
Cows
Immunization
Vaccines
Bovine Mastitis
Recombinant Proteins
Mastítis Bovina - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/11331
Ver los metadatos del registro completo
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Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cowsCamussone, CeciliaReidel, Ivana GabrielaMolineri, Ana InesCicotello, JoaquinMiotti, CamilaSuarez Archilla, GuillermoCurti, C.C.Veaute, Carolina Melania IsabelCalvinho, Luis FernandoNovillaVacaStaphylococcus aureusInmunizaciónVacunaProteínas RecombinantesHeifersCowsImmunizationVaccinesBovine MastitisRecombinant ProteinsMastítis BovinaThe aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation.EEA RafaelaFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; ArgentinaFil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.Fil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cicotello, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Cicotello, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Curti, C.C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; ArgentinaFil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2023-03-072022-03-07T14:02:23Z2022-03-07T14:02:23Z2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11331https://www.sciencedirect.com/science/article/pii/S00345288220005100034-5288https://doi.org/10.1016/j.rvsc.2022.02.014Research in Veterinary Science 145 : 177-187 (July 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud públicainfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:29Zoai:localhost:20.500.12123/11331instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:30.053INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| title |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| spellingShingle |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows Camussone, Cecilia Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina |
| title_short |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| title_full |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| title_fullStr |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| title_full_unstemmed |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| title_sort |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
| dc.creator.none.fl_str_mv |
Camussone, Cecilia Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando |
| author |
Camussone, Cecilia |
| author_facet |
Camussone, Cecilia Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando |
| author_role |
author |
| author2 |
Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina |
| topic |
Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina |
| dc.description.none.fl_txt_mv |
The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation. EEA Rafaela Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina Fil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cicotello, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Cicotello, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Curti, C.C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina. Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-03-07T14:02:23Z 2022-03-07T14:02:23Z 2022-07 info:eu-repo/date/embargoEnd/2023-03-07 |
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info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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acceptedVersion |
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http://hdl.handle.net/20.500.12123/11331 https://www.sciencedirect.com/science/article/pii/S0034528822000510 0034-5288 https://doi.org/10.1016/j.rvsc.2022.02.014 |
| url |
http://hdl.handle.net/20.500.12123/11331 https://www.sciencedirect.com/science/article/pii/S0034528822000510 https://doi.org/10.1016/j.rvsc.2022.02.014 |
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0034-5288 |
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eng |
| language |
eng |
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info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública |
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Elsevier |
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Elsevier |
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Research in Veterinary Science 145 : 177-187 (July 2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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