Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions

Autores
Setten, Lorena; Soto, Gabriela Cinthia; Mozzicafreddo, Matteo; Fox, Ana Romina; Lisi, Christian Daniel; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Diaz Paleo, Antonio Horacio; Ayub, Nicolás Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.
Fil: Setten, Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mozzicafreddo, Matteo. Università di Camerino. School of Biosciences and Biotechnology; Italia
Fil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lisi, Christian Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Cuccioloni, Massimiliano. Università di Camerino. School of Biosciences and Biotechnology; Italia
Fil: Angeletti, Mauro. Università di Camerino. School of Biosciences and Biotechnology; Italia
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
PLoS ONE 8 (5) : e63666. (2013)
Materia
Pseudomonas
Microorganismos
Organismos Modificados Genéticamente
Bacteria Fijadora del Nitrógeno
Fijación del Nitrógeno
Fijación del Amonio
Nitrogenasa
Rizosfera
Nitrógeno Asimilable
Inoculación
Inoculation
Arabidopsis
Biotecnología Vegetal
Sustancias de Crecimiento Vegetal
Deficiencias del Suelo
Microorganisms
Genetically Modified Organisms
Nitrogen Fixing Bacteria
Nitrogen Fixation
Ammonium Fixation
Nitrogenase
Rhizosphere
Digestible Nitrogen
Plant Biotechnology
Soil Deficiencies
Argentina
Diazotrofos Endofitos
Bacterias Recombinantes
Pseudomonas Protogens Pf5
Fijación Biológica del Nitrógeno
Deficiencias de Nitrógeno
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1450
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oai_identifier_str oai:localhost:20.500.12123/1450
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network_name_str INTA Digital (INTA)
spelling Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditionsSetten, LorenaSoto, Gabriela CinthiaMozzicafreddo, MatteoFox, Ana RominaLisi, Christian DanielCuccioloni, MassimilianoAngeletti, MauroPagano, Elba MariaDiaz Paleo, Antonio HoracioAyub, Nicolás DanielPseudomonasMicroorganismosOrganismos Modificados GenéticamenteBacteria Fijadora del NitrógenoFijación del NitrógenoFijación del AmonioNitrogenasaRizosferaNitrógeno AsimilableInoculaciónInoculationArabidopsisBiotecnología VegetalSustancias de Crecimiento VegetalDeficiencias del SueloMicroorganismsGenetically Modified OrganismsNitrogen Fixing BacteriaNitrogen FixationAmmonium FixationNitrogenaseRhizosphereDigestible NitrogenPlant BiotechnologySoil DeficienciesArgentinaDiazotrofos EndofitosBacterias RecombinantesPseudomonas Protogens Pf5Fijación Biológica del NitrógenoDeficiencias de NitrógenoNitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.Fil: Setten, Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mozzicafreddo, Matteo. Università di Camerino. School of Biosciences and Biotechnology; ItaliaFil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lisi, Christian Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Cuccioloni, Massimiliano. Università di Camerino. School of Biosciences and Biotechnology; ItaliaFil: Angeletti, Mauro. Università di Camerino. School of Biosciences and Biotechnology; ItaliaFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-10-10T14:32:22Z2017-10-10T14:32:22Z2013-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1450http://journals.plos.org/plosone/article?id=10.1371/journal.pone.00636661932-6203https://doi.org/10.1371/journal.pone.0063666PLoS ONE 8 (5) : e63666. (2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:05Zoai:localhost:20.500.12123/1450instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:06.298INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
title Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
spellingShingle Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
Setten, Lorena
Pseudomonas
Microorganismos
Organismos Modificados Genéticamente
Bacteria Fijadora del Nitrógeno
Fijación del Nitrógeno
Fijación del Amonio
Nitrogenasa
Rizosfera
Nitrógeno Asimilable
Inoculación
Inoculation
Arabidopsis
Biotecnología Vegetal
Sustancias de Crecimiento Vegetal
Deficiencias del Suelo
Microorganisms
Genetically Modified Organisms
Nitrogen Fixing Bacteria
Nitrogen Fixation
Ammonium Fixation
Nitrogenase
Rhizosphere
Digestible Nitrogen
Plant Biotechnology
Soil Deficiencies
Argentina
Diazotrofos Endofitos
Bacterias Recombinantes
Pseudomonas Protogens Pf5
Fijación Biológica del Nitrógeno
Deficiencias de Nitrógeno
title_short Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
title_full Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
title_fullStr Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
title_full_unstemmed Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
title_sort Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
dc.creator.none.fl_str_mv Setten, Lorena
Soto, Gabriela Cinthia
Mozzicafreddo, Matteo
Fox, Ana Romina
Lisi, Christian Daniel
Cuccioloni, Massimiliano
Angeletti, Mauro
Pagano, Elba Maria
Diaz Paleo, Antonio Horacio
Ayub, Nicolás Daniel
author Setten, Lorena
author_facet Setten, Lorena
Soto, Gabriela Cinthia
Mozzicafreddo, Matteo
Fox, Ana Romina
Lisi, Christian Daniel
Cuccioloni, Massimiliano
Angeletti, Mauro
Pagano, Elba Maria
Diaz Paleo, Antonio Horacio
Ayub, Nicolás Daniel
author_role author
author2 Soto, Gabriela Cinthia
Mozzicafreddo, Matteo
Fox, Ana Romina
Lisi, Christian Daniel
Cuccioloni, Massimiliano
Angeletti, Mauro
Pagano, Elba Maria
Diaz Paleo, Antonio Horacio
Ayub, Nicolás Daniel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pseudomonas
Microorganismos
Organismos Modificados Genéticamente
Bacteria Fijadora del Nitrógeno
Fijación del Nitrógeno
Fijación del Amonio
Nitrogenasa
Rizosfera
Nitrógeno Asimilable
Inoculación
Inoculation
Arabidopsis
Biotecnología Vegetal
Sustancias de Crecimiento Vegetal
Deficiencias del Suelo
Microorganisms
Genetically Modified Organisms
Nitrogen Fixing Bacteria
Nitrogen Fixation
Ammonium Fixation
Nitrogenase
Rhizosphere
Digestible Nitrogen
Plant Biotechnology
Soil Deficiencies
Argentina
Diazotrofos Endofitos
Bacterias Recombinantes
Pseudomonas Protogens Pf5
Fijación Biológica del Nitrógeno
Deficiencias de Nitrógeno
topic Pseudomonas
Microorganismos
Organismos Modificados Genéticamente
Bacteria Fijadora del Nitrógeno
Fijación del Nitrógeno
Fijación del Amonio
Nitrogenasa
Rizosfera
Nitrógeno Asimilable
Inoculación
Inoculation
Arabidopsis
Biotecnología Vegetal
Sustancias de Crecimiento Vegetal
Deficiencias del Suelo
Microorganisms
Genetically Modified Organisms
Nitrogen Fixing Bacteria
Nitrogen Fixation
Ammonium Fixation
Nitrogenase
Rhizosphere
Digestible Nitrogen
Plant Biotechnology
Soil Deficiencies
Argentina
Diazotrofos Endofitos
Bacterias Recombinantes
Pseudomonas Protogens Pf5
Fijación Biológica del Nitrógeno
Deficiencias de Nitrógeno
dc.description.none.fl_txt_mv Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.
Fil: Setten, Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mozzicafreddo, Matteo. Università di Camerino. School of Biosciences and Biotechnology; Italia
Fil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lisi, Christian Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Cuccioloni, Massimiliano. Università di Camerino. School of Biosciences and Biotechnology; Italia
Fil: Angeletti, Mauro. Università di Camerino. School of Biosciences and Biotechnology; Italia
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-13
2017-10-10T14:32:22Z
2017-10-10T14:32:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1450
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666
1932-6203
https://doi.org/10.1371/journal.pone.0063666
url http://hdl.handle.net/20.500.12123/1450
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063666
https://doi.org/10.1371/journal.pone.0063666
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 8 (5) : e63666. (2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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