Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential
- Autores
- Salgueiro, Julieta; Nussenbaum, Ana Laura; Marchesini, María Inés; Garbalena, Micaela Soledad; Brambilla, Silvina Maricel; Belliard, Silvina Ahnahi; Cuadros, Fabián; Núñez, Mauricio; Yáñez, Carolina; Juárez, María Laura; Vera, María Teresa; Lanzavecchia, Silvia Beatriz; Tsiamis, George; Segura, Diego Fernando
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). The presence of diazotrophic bacteria in A. fraterculus sp. 1 has been demonstrated through molecular, culture-independent methods. This study is aimed to characterize the composition and diversity of culturable gut bacteria of A. fraterculus sp. 1 males from different origins, and explore their metabolic roles, focusing on diazotrophic bacteria. Three male groups were studied: wild-caught (WW), lab-reared from wild larvae (WL), and lab-colony raised (LL). Gut bacteria were collected and characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL and NFb). Phylogenetic analysis of 16S rRNA gene mapped potential diazotrophs across the bacterial collection, while biochemical profiling and ARDRA (Amplified rDNA Restriction Analysis) were used to quickly differentiate diazotrophic bacteria. PCR testing for the nifH gene, associated with nitrogen fixation, was also performed. Bacterial diversity was highest in WW, followed by WL, and lowest in LL. In LL and WL, Enterobacter was the most frequent genus, while Klebsiella dominated in WW. Among the 20 SIL+ isolates identified, 10 came from WW, 9 from WL, and 1 from LL. One of these isolates (Enterobacter sp.) was tested as a supplement to the adult diet, without showing a beneficial effect on males pheromone calling behavior. Three isolates were also NFb+; two had the nifH gene. ARDRA was effective for rapid diazotroph discrimination. These findings highlight the potential of gut symbiotic bacteria in eco-friendly pest management strategies like the sterile insect technique (SIT). By using diazotrophic bacteria, protein requirements in artificial diets could be reduced, cutting costs and improving the affordability of SIT programs.
Instituto de Genética
Fil: Salgueiro, Julieta. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); Argentina
Fil: Salgueiro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); Argentina
Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; Argentina
Fil: Marchesini, María Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Marchesini, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garbalena, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Brambilla, Silvina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Belliard, Silvina Ahnahi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental (DBBE); Argentina
Fil: Cuadros, Fabián. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile
Fil: Núñez, Mauricio. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile
Fil: Yáñez, Carolina. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile
Fil: Juárez, María Laura. Fundación Miguel Lillo. Unidad Ejecutora Lillo; Argentina
Fil: Juárez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vera, María Teresa. Joint FAO/IAEA Center of Nuclear Techniques in Food and Agriculture. Insect Pest Control Laboratory; Austria
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tsiamis, George. University of Patras. Department of Environmental Engineering; Grecia
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; Argentina - Fuente
- Insect Science : 1-20 (First published: 24 February 2025)
- Materia
-
Anastrepha fraterculus
Nitrogen Fixation
Symbionts
Nitrogen-fixing Bacteria
Tephritidae
Sterile Insect Release
Bacteria
Fijación del Nitrógeno
Simbiontico
Bacteria Fijadora del Nitrógeno
Liberación de Insectos Estériles - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21545
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Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potentialSalgueiro, JulietaNussenbaum, Ana LauraMarchesini, María InésGarbalena, Micaela SoledadBrambilla, Silvina MaricelBelliard, Silvina AhnahiCuadros, FabiánNúñez, MauricioYáñez, CarolinaJuárez, María LauraVera, María TeresaLanzavecchia, Silvia BeatrizTsiamis, GeorgeSegura, Diego FernandoAnastrepha fraterculusNitrogen FixationSymbiontsNitrogen-fixing BacteriaTephritidaeSterile Insect ReleaseBacteriaFijación del NitrógenoSimbionticoBacteria Fijadora del NitrógenoLiberación de Insectos EstérilesAnastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). The presence of diazotrophic bacteria in A. fraterculus sp. 1 has been demonstrated through molecular, culture-independent methods. This study is aimed to characterize the composition and diversity of culturable gut bacteria of A. fraterculus sp. 1 males from different origins, and explore their metabolic roles, focusing on diazotrophic bacteria. Three male groups were studied: wild-caught (WW), lab-reared from wild larvae (WL), and lab-colony raised (LL). Gut bacteria were collected and characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL and NFb). Phylogenetic analysis of 16S rRNA gene mapped potential diazotrophs across the bacterial collection, while biochemical profiling and ARDRA (Amplified rDNA Restriction Analysis) were used to quickly differentiate diazotrophic bacteria. PCR testing for the nifH gene, associated with nitrogen fixation, was also performed. Bacterial diversity was highest in WW, followed by WL, and lowest in LL. In LL and WL, Enterobacter was the most frequent genus, while Klebsiella dominated in WW. Among the 20 SIL+ isolates identified, 10 came from WW, 9 from WL, and 1 from LL. One of these isolates (Enterobacter sp.) was tested as a supplement to the adult diet, without showing a beneficial effect on males pheromone calling behavior. Three isolates were also NFb+; two had the nifH gene. ARDRA was effective for rapid diazotroph discrimination. These findings highlight the potential of gut symbiotic bacteria in eco-friendly pest management strategies like the sterile insect technique (SIT). By using diazotrophic bacteria, protein requirements in artificial diets could be reduced, cutting costs and improving the affordability of SIT programs.Instituto de GenéticaFil: Salgueiro, Julieta. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); ArgentinaFil: Salgueiro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); ArgentinaFil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; ArgentinaFil: Marchesini, María Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Marchesini, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Garbalena, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Brambilla, Silvina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Belliard, Silvina Ahnahi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental (DBBE); ArgentinaFil: Cuadros, Fabián. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; ChileFil: Núñez, Mauricio. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; ChileFil: Yáñez, Carolina. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; ChileFil: Juárez, María Laura. Fundación Miguel Lillo. Unidad Ejecutora Lillo; ArgentinaFil: Juárez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vera, María Teresa. Joint FAO/IAEA Center of Nuclear Techniques in Food and Agriculture. Insect Pest Control Laboratory; AustriaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tsiamis, George. University of Patras. Department of Environmental Engineering; GreciaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; ArgentinaWiley2025-03-05T14:43:30Z2025-03-05T14:43:30Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21545https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.700041744-7917https://doi.org/10.1111/1744-7917.70004Insect Science : 1-20 (First published: 24 February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:25Zoai:localhost:20.500.12123/21545instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:26.144INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| spellingShingle |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential Salgueiro, Julieta Anastrepha fraterculus Nitrogen Fixation Symbionts Nitrogen-fixing Bacteria Tephritidae Sterile Insect Release Bacteria Fijación del Nitrógeno Simbiontico Bacteria Fijadora del Nitrógeno Liberación de Insectos Estériles |
| title_short |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_full |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_fullStr |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_full_unstemmed |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_sort |
Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| dc.creator.none.fl_str_mv |
Salgueiro, Julieta Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author |
Salgueiro, Julieta |
| author_facet |
Salgueiro, Julieta Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author_role |
author |
| author2 |
Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Anastrepha fraterculus Nitrogen Fixation Symbionts Nitrogen-fixing Bacteria Tephritidae Sterile Insect Release Bacteria Fijación del Nitrógeno Simbiontico Bacteria Fijadora del Nitrógeno Liberación de Insectos Estériles |
| topic |
Anastrepha fraterculus Nitrogen Fixation Symbionts Nitrogen-fixing Bacteria Tephritidae Sterile Insect Release Bacteria Fijación del Nitrógeno Simbiontico Bacteria Fijadora del Nitrógeno Liberación de Insectos Estériles |
| dc.description.none.fl_txt_mv |
Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). The presence of diazotrophic bacteria in A. fraterculus sp. 1 has been demonstrated through molecular, culture-independent methods. This study is aimed to characterize the composition and diversity of culturable gut bacteria of A. fraterculus sp. 1 males from different origins, and explore their metabolic roles, focusing on diazotrophic bacteria. Three male groups were studied: wild-caught (WW), lab-reared from wild larvae (WL), and lab-colony raised (LL). Gut bacteria were collected and characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL and NFb). Phylogenetic analysis of 16S rRNA gene mapped potential diazotrophs across the bacterial collection, while biochemical profiling and ARDRA (Amplified rDNA Restriction Analysis) were used to quickly differentiate diazotrophic bacteria. PCR testing for the nifH gene, associated with nitrogen fixation, was also performed. Bacterial diversity was highest in WW, followed by WL, and lowest in LL. In LL and WL, Enterobacter was the most frequent genus, while Klebsiella dominated in WW. Among the 20 SIL+ isolates identified, 10 came from WW, 9 from WL, and 1 from LL. One of these isolates (Enterobacter sp.) was tested as a supplement to the adult diet, without showing a beneficial effect on males pheromone calling behavior. Three isolates were also NFb+; two had the nifH gene. ARDRA was effective for rapid diazotroph discrimination. These findings highlight the potential of gut symbiotic bacteria in eco-friendly pest management strategies like the sterile insect technique (SIT). By using diazotrophic bacteria, protein requirements in artificial diets could be reduced, cutting costs and improving the affordability of SIT programs. Instituto de Genética Fil: Salgueiro, Julieta. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); Argentina Fil: Salgueiro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; Argentina Fil: Marchesini, María Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Marchesini, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina Fil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garbalena, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Brambilla, Silvina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Belliard, Silvina Ahnahi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental (DBBE); Argentina Fil: Cuadros, Fabián. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile Fil: Núñez, Mauricio. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile Fil: Yáñez, Carolina. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile Fil: Juárez, María Laura. Fundación Miguel Lillo. Unidad Ejecutora Lillo; Argentina Fil: Juárez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vera, María Teresa. Joint FAO/IAEA Center of Nuclear Techniques in Food and Agriculture. Insect Pest Control Laboratory; Austria Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tsiamis, George. University of Patras. Department of Environmental Engineering; Grecia Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; Argentina |
| description |
Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). The presence of diazotrophic bacteria in A. fraterculus sp. 1 has been demonstrated through molecular, culture-independent methods. This study is aimed to characterize the composition and diversity of culturable gut bacteria of A. fraterculus sp. 1 males from different origins, and explore their metabolic roles, focusing on diazotrophic bacteria. Three male groups were studied: wild-caught (WW), lab-reared from wild larvae (WL), and lab-colony raised (LL). Gut bacteria were collected and characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL and NFb). Phylogenetic analysis of 16S rRNA gene mapped potential diazotrophs across the bacterial collection, while biochemical profiling and ARDRA (Amplified rDNA Restriction Analysis) were used to quickly differentiate diazotrophic bacteria. PCR testing for the nifH gene, associated with nitrogen fixation, was also performed. Bacterial diversity was highest in WW, followed by WL, and lowest in LL. In LL and WL, Enterobacter was the most frequent genus, while Klebsiella dominated in WW. Among the 20 SIL+ isolates identified, 10 came from WW, 9 from WL, and 1 from LL. One of these isolates (Enterobacter sp.) was tested as a supplement to the adult diet, without showing a beneficial effect on males pheromone calling behavior. Three isolates were also NFb+; two had the nifH gene. ARDRA was effective for rapid diazotroph discrimination. These findings highlight the potential of gut symbiotic bacteria in eco-friendly pest management strategies like the sterile insect technique (SIT). By using diazotrophic bacteria, protein requirements in artificial diets could be reduced, cutting costs and improving the affordability of SIT programs. |
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2025 |
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