In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)

Autores
Carloni, Edgardo José; Rodriguez, Milena Nahir; Verga, Anibal; Grunberg, Karina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Verga, Anibal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Rioja. Agencia de Extensión Rural La Rioja; Argentina
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fuente
Plant Cell, Tissue and Organ Culture (PCTOC) 161 : 77. (Published: 28 May 2025)
Materia
Vegetative Propagation
Forestry
Propagación Vegetativa
Ciencia forestal
Prosopis alba
Plant Tissue Culture
Micrografting
Rejuvenation
Rhizogenesis
Algarrobo blanco
Neltuma alba
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22572
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spelling In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)Carloni, Edgardo JoséRodriguez, Milena NahirVerga, AnibalGrunberg, KarinaVegetative PropagationForestryPropagación VegetativaCiencia forestalProsopis albaPlant Tissue CultureMicrograftingRejuvenationRhizogenesisAlgarrobo blancoNeltuma albaNeltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Verga, Anibal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Rioja. Agencia de Extensión Rural La Rioja; ArgentinaFil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaSpringer2025-06-09T10:03:27Z2025-06-09T10:03:27Z2025-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22572https://link.springer.com/article/10.1007/s11240-025-03107-10167-68571573-5044https://doi.org/10.1007/s11240-025-03107-1Plant Cell, Tissue and Organ Culture (PCTOC) 161 : 77. (Published: 28 May 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001, Multiplicación de propágulos de sanidad controladainfo:eu-repograntAgreement/INTA/2023-PE-L01-I067, Mejoramiento genético y silvicultura de plantaciones para la producción sostenible de productos forestales para distintos destinos industrialesinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:19Zoai:localhost:20.500.12123/22572instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:20.112INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
title In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
spellingShingle In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
Carloni, Edgardo José
Vegetative Propagation
Forestry
Propagación Vegetativa
Ciencia forestal
Prosopis alba
Plant Tissue Culture
Micrografting
Rejuvenation
Rhizogenesis
Algarrobo blanco
Neltuma alba
title_short In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
title_full In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
title_fullStr In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
title_full_unstemmed In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
title_sort In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
dc.creator.none.fl_str_mv Carloni, Edgardo José
Rodriguez, Milena Nahir
Verga, Anibal
Grunberg, Karina
author Carloni, Edgardo José
author_facet Carloni, Edgardo José
Rodriguez, Milena Nahir
Verga, Anibal
Grunberg, Karina
author_role author
author2 Rodriguez, Milena Nahir
Verga, Anibal
Grunberg, Karina
author2_role author
author
author
dc.subject.none.fl_str_mv Vegetative Propagation
Forestry
Propagación Vegetativa
Ciencia forestal
Prosopis alba
Plant Tissue Culture
Micrografting
Rejuvenation
Rhizogenesis
Algarrobo blanco
Neltuma alba
topic Vegetative Propagation
Forestry
Propagación Vegetativa
Ciencia forestal
Prosopis alba
Plant Tissue Culture
Micrografting
Rejuvenation
Rhizogenesis
Algarrobo blanco
Neltuma alba
dc.description.none.fl_txt_mv Neltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Verga, Anibal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Rioja. Agencia de Extensión Rural La Rioja; Argentina
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
description Neltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-09T10:03:27Z
2025-06-09T10:03:27Z
2025-05-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22572
https://link.springer.com/article/10.1007/s11240-025-03107-1
0167-6857
1573-5044
https://doi.org/10.1007/s11240-025-03107-1
url http://hdl.handle.net/20.500.12123/22572
https://link.springer.com/article/10.1007/s11240-025-03107-1
https://doi.org/10.1007/s11240-025-03107-1
identifier_str_mv 0167-6857
1573-5044
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001, Multiplicación de propágulos de sanidad controlada
info:eu-repograntAgreement/INTA/2023-PE-L01-I067, Mejoramiento genético y silvicultura de plantaciones para la producción sostenible de productos forestales para distintos destinos industriales
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant Cell, Tissue and Organ Culture (PCTOC) 161 : 77. (Published: 28 May 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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