Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle

Autores
Hidalgo-Ruiz, Mario; Mejia-López, Susana; Pérez-Serrano, Rosa M.; Zaldívar-Lelo de Larrea, Guadalupe; Ganzinelli, Sabrina Belen; Florin-Christensen, Monica; Suarez, Carlos Esteban; Hernández-Ortiz, Rubén; Mercado-Uriostegui, Miguel A.; Rodríguez-Torres, Angelina; Carvajal-Gamez, Bertha I.; Camacho-Nuez, Minerva; Wilkowsky, Silvina Elizabeth; Mosqueda, Juan
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis.
Instituto de Patobiología
Fil: Hidalgo-Ruiz, Mario. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Mejia-López, Susana. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Pérez-Serrano, Rosa M. Universidad Autónoma de Querétaro. Facultad de Medicina; México
Fil: Zaldívar-Lelo de Larrea, Guadalupe. Universidad Autónoma de Querétaro. Facultad de Medicina; México
Fil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina
Fil: Ganzinelli Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina
Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidos
Fil: Hernández-Ortiz, Rubén. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Centro de Estudios e Investigaciones para el Desarrollo Docente (CENID). Salud Animal e Inocuidad; México
Fil: Mercado-Uriostegui, Miguel A. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Rodríguez-Torres, Angelina. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Carvajal-Gamez, Bertha I. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Camacho-Nuez, Minerva. Universidad Autónoma de la Ciudad de México. Posgrado en Ciencias Genómicas; México
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mosqueda, Juan. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fuente
Vaccine 40 (8) : 1108-1115 (Febrero 2022)
Materia
Babesia bovis
Linfocitos-t
Interferonas
Anticuerpos
Respuesta Inmunológica
Neutralización
T-lymphocytes
Interferons
Antibodies
Immune Response
Neutralization
T-cell
Células-t
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11574
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spelling Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattleHidalgo-Ruiz, MarioMejia-López, SusanaPérez-Serrano, Rosa M.Zaldívar-Lelo de Larrea, GuadalupeGanzinelli, Sabrina BelenFlorin-Christensen, MonicaSuarez, Carlos EstebanHernández-Ortiz, RubénMercado-Uriostegui, Miguel A.Rodríguez-Torres, AngelinaCarvajal-Gamez, Bertha I.Camacho-Nuez, MinervaWilkowsky, Silvina ElizabethMosqueda, JuanBabesia bovisLinfocitos-tInterferonasAnticuerposRespuesta InmunológicaNeutralizaciónT-lymphocytesInterferonsAntibodiesImmune ResponseNeutralizationT-cellCélulas-tVaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis.Instituto de PatobiologíaFil: Hidalgo-Ruiz, Mario. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; MéxicoFil: Mejia-López, Susana. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; MéxicoFil: Pérez-Serrano, Rosa M. Universidad Autónoma de Querétaro. Facultad de Medicina; MéxicoFil: Zaldívar-Lelo de Larrea, Guadalupe. Universidad Autónoma de Querétaro. Facultad de Medicina; MéxicoFil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); ArgentinaFil: Ganzinelli Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); ArgentinaFil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados UnidosFil: Hernández-Ortiz, Rubén. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Centro de Estudios e Investigaciones para el Desarrollo Docente (CENID). Salud Animal e Inocuidad; MéxicoFil: Mercado-Uriostegui, Miguel A. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; MéxicoFil: Rodríguez-Torres, Angelina. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; MéxicoFil: Carvajal-Gamez, Bertha I. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; MéxicoFil: Camacho-Nuez, Minerva. Universidad Autónoma de la Ciudad de México. Posgrado en Ciencias Genómicas; MéxicoFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Mosqueda, Juan. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; MéxicoElsevier2022-04-07T10:43:44Z2022-04-07T10:43:44Z2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11574https://www.sciencedirect.com/science/article/pii/S0264410X220004940264-410Xhttps://doi.org/10.1016/j.vaccine.2022.01.023Vaccine 40 (8) : 1108-1115 (Febrero 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:31Zoai:localhost:20.500.12123/11574instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:31.698INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
title Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
spellingShingle Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
Hidalgo-Ruiz, Mario
Babesia bovis
Linfocitos-t
Interferonas
Anticuerpos
Respuesta Inmunológica
Neutralización
T-lymphocytes
Interferons
Antibodies
Immune Response
Neutralization
T-cell
Células-t
title_short Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
title_full Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
title_fullStr Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
title_full_unstemmed Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
title_sort Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
dc.creator.none.fl_str_mv Hidalgo-Ruiz, Mario
Mejia-López, Susana
Pérez-Serrano, Rosa M.
Zaldívar-Lelo de Larrea, Guadalupe
Ganzinelli, Sabrina Belen
Florin-Christensen, Monica
Suarez, Carlos Esteban
Hernández-Ortiz, Rubén
Mercado-Uriostegui, Miguel A.
Rodríguez-Torres, Angelina
Carvajal-Gamez, Bertha I.
Camacho-Nuez, Minerva
Wilkowsky, Silvina Elizabeth
Mosqueda, Juan
author Hidalgo-Ruiz, Mario
author_facet Hidalgo-Ruiz, Mario
Mejia-López, Susana
Pérez-Serrano, Rosa M.
Zaldívar-Lelo de Larrea, Guadalupe
Ganzinelli, Sabrina Belen
Florin-Christensen, Monica
Suarez, Carlos Esteban
Hernández-Ortiz, Rubén
Mercado-Uriostegui, Miguel A.
Rodríguez-Torres, Angelina
Carvajal-Gamez, Bertha I.
Camacho-Nuez, Minerva
Wilkowsky, Silvina Elizabeth
Mosqueda, Juan
author_role author
author2 Mejia-López, Susana
Pérez-Serrano, Rosa M.
Zaldívar-Lelo de Larrea, Guadalupe
Ganzinelli, Sabrina Belen
Florin-Christensen, Monica
Suarez, Carlos Esteban
Hernández-Ortiz, Rubén
Mercado-Uriostegui, Miguel A.
Rodríguez-Torres, Angelina
Carvajal-Gamez, Bertha I.
Camacho-Nuez, Minerva
Wilkowsky, Silvina Elizabeth
Mosqueda, Juan
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia bovis
Linfocitos-t
Interferonas
Anticuerpos
Respuesta Inmunológica
Neutralización
T-lymphocytes
Interferons
Antibodies
Immune Response
Neutralization
T-cell
Células-t
topic Babesia bovis
Linfocitos-t
Interferonas
Anticuerpos
Respuesta Inmunológica
Neutralización
T-lymphocytes
Interferons
Antibodies
Immune Response
Neutralization
T-cell
Células-t
dc.description.none.fl_txt_mv Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis.
Instituto de Patobiología
Fil: Hidalgo-Ruiz, Mario. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Mejia-López, Susana. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Pérez-Serrano, Rosa M. Universidad Autónoma de Querétaro. Facultad de Medicina; México
Fil: Zaldívar-Lelo de Larrea, Guadalupe. Universidad Autónoma de Querétaro. Facultad de Medicina; México
Fil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina
Fil: Ganzinelli Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina
Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidos
Fil: Hernández-Ortiz, Rubén. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Centro de Estudios e Investigaciones para el Desarrollo Docente (CENID). Salud Animal e Inocuidad; México
Fil: Mercado-Uriostegui, Miguel A. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Rodríguez-Torres, Angelina. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Carvajal-Gamez, Bertha I. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
Fil: Camacho-Nuez, Minerva. Universidad Autónoma de la Ciudad de México. Posgrado en Ciencias Genómicas; México
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mosqueda, Juan. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México
description Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-07T10:43:44Z
2022-04-07T10:43:44Z
2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11574
https://www.sciencedirect.com/science/article/pii/S0264410X22000494
0264-410X
https://doi.org/10.1016/j.vaccine.2022.01.023
url http://hdl.handle.net/20.500.12123/11574
https://www.sciencedirect.com/science/article/pii/S0264410X22000494
https://doi.org/10.1016/j.vaccine.2022.01.023
identifier_str_mv 0264-410X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Vaccine 40 (8) : 1108-1115 (Febrero 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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