Urinary bladder lesions of bovine enzootic hematuria in Argentina

Autores
Aguirre Castro, Laura Sabrina; Barbeito, Claudio Gustavo; Medina, Diego Martín Nicolás; Colque Caro, Luis Adrián; Micheloud, Juan Francisco
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer.
Instituto de Investigación Animal del Chaco Semiárido
Fil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguirre Castro, Laura Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Aguirre Castro, Laura Sabrina. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada; Argentina
Fil: Medina, Diego Martín Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Diego Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Medina, Diego Martín Nicolás. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fuente
Research in Veterinary Science 193 : 105797. (September 2025)
Materia
Ganado Bovino
Enfermedades del Tracto Urinario
Hematuria
Plantas Tóxicas
Argentina
Cattle
Urinary Tract Diseases
Haematuria
Poisonous Plants
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23062
Acceder
id INTADig_93a741ed998800539d3cfa516ae2fe13
oai_identifier_str oai:localhost:20.500.12123/23062
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Urinary bladder lesions of bovine enzootic hematuria in ArgentinaAguirre Castro, Laura SabrinaBarbeito, Claudio GustavoMedina, Diego Martín NicolásColque Caro, Luis AdriánMicheloud, Juan FranciscoGanado BovinoEnfermedades del Tracto UrinarioHematuriaPlantas TóxicasArgentinaCattleUrinary Tract DiseasesHaematuriaPoisonous PlantsBovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer.Instituto de Investigación Animal del Chaco SemiáridoFil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aguirre Castro, Laura Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; ArgentinaFil: Aguirre Castro, Laura Sabrina. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaFil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada; ArgentinaFil: Medina, Diego Martín Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medina, Diego Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; ArgentinaFil: Medina, Diego Martín Nicolás. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaFil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; ArgentinaFil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; ArgentinaFil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaElsevier2025-07-17T13:28:51Z2025-07-17T13:28:51Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23062https://www.sciencedirect.com/science/article/abs/pii/S00345288250027110034-52881532-2661https://doi.org/10.1016/j.rvsc.2025.105797Research in Veterinary Science 193 : 105797. (September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:25Zoai:localhost:20.500.12123/23062instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:25.474INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Urinary bladder lesions of bovine enzootic hematuria in Argentina
title Urinary bladder lesions of bovine enzootic hematuria in Argentina
spellingShingle Urinary bladder lesions of bovine enzootic hematuria in Argentina
Aguirre Castro, Laura Sabrina
Ganado Bovino
Enfermedades del Tracto Urinario
Hematuria
Plantas Tóxicas
Argentina
Cattle
Urinary Tract Diseases
Haematuria
Poisonous Plants
title_short Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_full Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_fullStr Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_full_unstemmed Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_sort Urinary bladder lesions of bovine enzootic hematuria in Argentina
dc.creator.none.fl_str_mv Aguirre Castro, Laura Sabrina
Barbeito, Claudio Gustavo
Medina, Diego Martín Nicolás
Colque Caro, Luis Adrián
Micheloud, Juan Francisco
author Aguirre Castro, Laura Sabrina
author_facet Aguirre Castro, Laura Sabrina
Barbeito, Claudio Gustavo
Medina, Diego Martín Nicolás
Colque Caro, Luis Adrián
Micheloud, Juan Francisco
author_role author
author2 Barbeito, Claudio Gustavo
Medina, Diego Martín Nicolás
Colque Caro, Luis Adrián
Micheloud, Juan Francisco
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades del Tracto Urinario
Hematuria
Plantas Tóxicas
Argentina
Cattle
Urinary Tract Diseases
Haematuria
Poisonous Plants
topic Ganado Bovino
Enfermedades del Tracto Urinario
Hematuria
Plantas Tóxicas
Argentina
Cattle
Urinary Tract Diseases
Haematuria
Poisonous Plants
dc.description.none.fl_txt_mv Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer.
Instituto de Investigación Animal del Chaco Semiárido
Fil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguirre Castro, Laura Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Aguirre Castro, Laura Sabrina. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada; Argentina
Fil: Medina, Diego Martín Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Diego Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Medina, Diego Martín Nicolás. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
description Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-17T13:28:51Z
2025-07-17T13:28:51Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23062
https://www.sciencedirect.com/science/article/abs/pii/S0034528825002711
0034-5288
1532-2661
https://doi.org/10.1016/j.rvsc.2025.105797
url http://hdl.handle.net/20.500.12123/23062
https://www.sciencedirect.com/science/article/abs/pii/S0034528825002711
https://doi.org/10.1016/j.rvsc.2025.105797
identifier_str_mv 0034-5288
1532-2661
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science 193 : 105797. (September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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