Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene

Autores
Tranquilli, Gabriela; Lewis, Silvina; Kippes, Nestor; Dubcovsky, Jorge; Alvarez, María Alejandra
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wheat cultivars exposed to optimal photoperiod and vernalization treatments still exhibit differences in heading time. This variation, known as earliness per se (Eps), is important for the fine-tuning of flowering and the adaptation to different environments. We previously identified the Eps-Am1 locus from Triticum monococcum and showed that the allele from cultivated accession DV92 significantly delays flowering and increases the number of spikelets per spike relative to the allele from wild accession G3116. We expanded a high-density genetic map and physical map of the Eps-Am1 region and identified the wheat ortholog of circadian clock regulator EARLY FLOWERING 3 (ELF3) as one of the candidate genes. No differences were found in ELF3 transcript levels between NILs carrying the DV92 and G3116 Eps-Am1 alleles, but the encoded ELF3 proteins differed in four amino acids. These differences were associated with altered transcription profiles of PIF-like, PPD1 and FT1, which are known downstream targets of ELF3. Tetraploid wheat lines with combined truncation mutations in the A- and B-genome copies of ELF3 flowered earlier and had less spikelets per spike than the wild type control under SD and LD conditions. Both effects were stronger in the photoperiod sensitive than the photoperiod insensitive background, indicating an epistatic interaction between PPD1 and ELF3. By contrast, the introgression of the T. monococcum chromosome segment carrying Eps-Am1-l allele from DV92 into durum wheat delayed flowering and increased the number of spikelets per spike in the field, providing a novel allele to modulate flowering time and spike development in wheat.
Inst. de Recursos Biológicos
Fil: Alvarez, María Alejandra. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Lewis, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Kippes, Nestor. University of California. Department of Plant Sciences; Estados Unidos
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fuente
Functional & Integrative Genomics 16 (4) : 365–382 (July 2016)
Materia
Triticum Monococcum
Trigo
Floración
Genes
Wheat
Flowering
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1703
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network_name_str INTA Digital (INTA)
spelling Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate geneTranquilli, GabrielaLewis, SilvinaKippes, NestorDubcovsky, JorgeAlvarez, María AlejandraTriticum MonococcumTrigoFloraciónGenesWheatFloweringWheat cultivars exposed to optimal photoperiod and vernalization treatments still exhibit differences in heading time. This variation, known as earliness per se (Eps), is important for the fine-tuning of flowering and the adaptation to different environments. We previously identified the Eps-Am1 locus from Triticum monococcum and showed that the allele from cultivated accession DV92 significantly delays flowering and increases the number of spikelets per spike relative to the allele from wild accession G3116. We expanded a high-density genetic map and physical map of the Eps-Am1 region and identified the wheat ortholog of circadian clock regulator EARLY FLOWERING 3 (ELF3) as one of the candidate genes. No differences were found in ELF3 transcript levels between NILs carrying the DV92 and G3116 Eps-Am1 alleles, but the encoded ELF3 proteins differed in four amino acids. These differences were associated with altered transcription profiles of PIF-like, PPD1 and FT1, which are known downstream targets of ELF3. Tetraploid wheat lines with combined truncation mutations in the A- and B-genome copies of ELF3 flowered earlier and had less spikelets per spike than the wild type control under SD and LD conditions. Both effects were stronger in the photoperiod sensitive than the photoperiod insensitive background, indicating an epistatic interaction between PPD1 and ELF3. By contrast, the introgression of the T. monococcum chromosome segment carrying Eps-Am1-l allele from DV92 into durum wheat delayed flowering and increased the number of spikelets per spike in the field, providing a novel allele to modulate flowering time and spike development in wheat.Inst. de Recursos BiológicosFil: Alvarez, María Alejandra. University of California. Department of Plant Sciences; Estados UnidosFil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Lewis, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Kippes, Nestor. University of California. Department of Plant Sciences; Estados UnidosFil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos2017-11-08T11:41:21Z2017-11-08T11:41:21Z2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1703https://link.springer.com/article/10.1007%2Fs10142-016-0490-31438-793X (Print)1438-7948 (Online)https://doi.org/10.1007/s10142-016-0490-3Functional & Integrative Genomics 16 (4) : 365–382 (July 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:04Zoai:localhost:20.500.12123/1703instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:04.423INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
title Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
spellingShingle Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
Tranquilli, Gabriela
Triticum Monococcum
Trigo
Floración
Genes
Wheat
Flowering
title_short Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
title_full Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
title_fullStr Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
title_full_unstemmed Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
title_sort Genetic and physical mapping of the earliness perse locus Eps - Am1 in Triticum monococcum identifies early flowering 3 (ELF3) as a candidate gene
dc.creator.none.fl_str_mv Tranquilli, Gabriela
Lewis, Silvina
Kippes, Nestor
Dubcovsky, Jorge
Alvarez, María Alejandra
author Tranquilli, Gabriela
author_facet Tranquilli, Gabriela
Lewis, Silvina
Kippes, Nestor
Dubcovsky, Jorge
Alvarez, María Alejandra
author_role author
author2 Lewis, Silvina
Kippes, Nestor
Dubcovsky, Jorge
Alvarez, María Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Triticum Monococcum
Trigo
Floración
Genes
Wheat
Flowering
topic Triticum Monococcum
Trigo
Floración
Genes
Wheat
Flowering
dc.description.none.fl_txt_mv Wheat cultivars exposed to optimal photoperiod and vernalization treatments still exhibit differences in heading time. This variation, known as earliness per se (Eps), is important for the fine-tuning of flowering and the adaptation to different environments. We previously identified the Eps-Am1 locus from Triticum monococcum and showed that the allele from cultivated accession DV92 significantly delays flowering and increases the number of spikelets per spike relative to the allele from wild accession G3116. We expanded a high-density genetic map and physical map of the Eps-Am1 region and identified the wheat ortholog of circadian clock regulator EARLY FLOWERING 3 (ELF3) as one of the candidate genes. No differences were found in ELF3 transcript levels between NILs carrying the DV92 and G3116 Eps-Am1 alleles, but the encoded ELF3 proteins differed in four amino acids. These differences were associated with altered transcription profiles of PIF-like, PPD1 and FT1, which are known downstream targets of ELF3. Tetraploid wheat lines with combined truncation mutations in the A- and B-genome copies of ELF3 flowered earlier and had less spikelets per spike than the wild type control under SD and LD conditions. Both effects were stronger in the photoperiod sensitive than the photoperiod insensitive background, indicating an epistatic interaction between PPD1 and ELF3. By contrast, the introgression of the T. monococcum chromosome segment carrying Eps-Am1-l allele from DV92 into durum wheat delayed flowering and increased the number of spikelets per spike in the field, providing a novel allele to modulate flowering time and spike development in wheat.
Inst. de Recursos Biológicos
Fil: Alvarez, María Alejandra. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Lewis, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Kippes, Nestor. University of California. Department of Plant Sciences; Estados Unidos
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
description Wheat cultivars exposed to optimal photoperiod and vernalization treatments still exhibit differences in heading time. This variation, known as earliness per se (Eps), is important for the fine-tuning of flowering and the adaptation to different environments. We previously identified the Eps-Am1 locus from Triticum monococcum and showed that the allele from cultivated accession DV92 significantly delays flowering and increases the number of spikelets per spike relative to the allele from wild accession G3116. We expanded a high-density genetic map and physical map of the Eps-Am1 region and identified the wheat ortholog of circadian clock regulator EARLY FLOWERING 3 (ELF3) as one of the candidate genes. No differences were found in ELF3 transcript levels between NILs carrying the DV92 and G3116 Eps-Am1 alleles, but the encoded ELF3 proteins differed in four amino acids. These differences were associated with altered transcription profiles of PIF-like, PPD1 and FT1, which are known downstream targets of ELF3. Tetraploid wheat lines with combined truncation mutations in the A- and B-genome copies of ELF3 flowered earlier and had less spikelets per spike than the wild type control under SD and LD conditions. Both effects were stronger in the photoperiod sensitive than the photoperiod insensitive background, indicating an epistatic interaction between PPD1 and ELF3. By contrast, the introgression of the T. monococcum chromosome segment carrying Eps-Am1-l allele from DV92 into durum wheat delayed flowering and increased the number of spikelets per spike in the field, providing a novel allele to modulate flowering time and spike development in wheat.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
2017-11-08T11:41:21Z
2017-11-08T11:41:21Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1703
https://link.springer.com/article/10.1007%2Fs10142-016-0490-3
1438-793X (Print)
1438-7948 (Online)
https://doi.org/10.1007/s10142-016-0490-3
url http://hdl.handle.net/20.500.12123/1703
https://link.springer.com/article/10.1007%2Fs10142-016-0490-3
https://doi.org/10.1007/s10142-016-0490-3
identifier_str_mv 1438-793X (Print)
1438-7948 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Functional & Integrative Genomics 16 (4) : 365–382 (July 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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