Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis

Autores
Mateos, Julieta Lisa; Madrigal, Pedro; Tsuda, Kenichi; Rawat, Vimal; Richter, René; Romera Branchat, Maida; Fornara, Fabio; Schneeberger, Korbinian; Krajewski, Pawel; Coupland, George
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: The initiation of flowering is an important developmental transition as it marks the beginning of the reproductive phase in plants. The MADS-box transcription factors (TFs) FLOWERING LOCUS C (FLC) and SHORT VEGETATIVE PHASE (SVP) form a complex to repress the expression of genes that initiate flowering in Arabidopsis. Both TFs play a central role in the regulatory network by conferring seasonal patterns of flowering. However, their interdependence and biological relevance when acting as a complex have not been extensively studied. RESULTS: We characterized the effects of both TFs individually and as a complex on flowering initiation using transcriptome profiling and DNA-binding occupancy. We find four major clusters regulating transcriptional responses, and that DNA binding scenarios are highly affected by the presence of the cognate partner. Remarkably, we identify genes whose regulation depends exclusively on simultaneous action of both proteins, thus distinguishing between the specificity of the SVP:FLC complex and that of each TF acting individually. The downstream targets of the SVP:FLC complex include a higher proportion of genes regulating floral induction, whereas those bound by either TF independently are biased towards floral development. Many genes involved in gibberellin-related processes are bound by the SVP:FLC complex, suggesting that direct regulation of gibberellin metabolism by FLC and SVP contributes to their effects on flowering. CONCLUSIONS: The regulatory codes controlled by SVP and FLC were deciphered at the genome-wide level revealing substantial flexibility based on dependent and independent DNA binding that may contribute to variation and robustness in the regulation of flowering.
Fil: Mateos, Julieta Lisa. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Madrigal, Pedro. Polish Academy of Sciences; Polonia
Fil: Tsuda, Kenichi. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Rawat, Vimal. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Richter, René. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Romera Branchat, Maida. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Fornara, Fabio. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Schneeberger, Korbinian. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Krajewski, Pawel. Polish Academy of Sciences; Polonia
Fil: Coupland, George. Max Planck Institute for Plant Breeding Research; Alemania
Materia
TRANSCRIPTION FACTOR
FLOWERING TIME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10491
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10491
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in ArabidopsisMateos, Julieta LisaMadrigal, PedroTsuda, KenichiRawat, VimalRichter, RenéRomera Branchat, MaidaFornara, FabioSchneeberger, KorbinianKrajewski, PawelCoupland, GeorgeTRANSCRIPTION FACTORFLOWERING TIMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1BACKGROUND: The initiation of flowering is an important developmental transition as it marks the beginning of the reproductive phase in plants. The MADS-box transcription factors (TFs) FLOWERING LOCUS C (FLC) and SHORT VEGETATIVE PHASE (SVP) form a complex to repress the expression of genes that initiate flowering in Arabidopsis. Both TFs play a central role in the regulatory network by conferring seasonal patterns of flowering. However, their interdependence and biological relevance when acting as a complex have not been extensively studied. RESULTS: We characterized the effects of both TFs individually and as a complex on flowering initiation using transcriptome profiling and DNA-binding occupancy. We find four major clusters regulating transcriptional responses, and that DNA binding scenarios are highly affected by the presence of the cognate partner. Remarkably, we identify genes whose regulation depends exclusively on simultaneous action of both proteins, thus distinguishing between the specificity of the SVP:FLC complex and that of each TF acting individually. The downstream targets of the SVP:FLC complex include a higher proportion of genes regulating floral induction, whereas those bound by either TF independently are biased towards floral development. Many genes involved in gibberellin-related processes are bound by the SVP:FLC complex, suggesting that direct regulation of gibberellin metabolism by FLC and SVP contributes to their effects on flowering. CONCLUSIONS: The regulatory codes controlled by SVP and FLC were deciphered at the genome-wide level revealing substantial flexibility based on dependent and independent DNA binding that may contribute to variation and robustness in the regulation of flowering.Fil: Mateos, Julieta Lisa. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Madrigal, Pedro. Polish Academy of Sciences; PoloniaFil: Tsuda, Kenichi. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Rawat, Vimal. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Richter, René. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Romera Branchat, Maida. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Fornara, Fabio. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Schneeberger, Korbinian. Max Planck Institute for Plant Breeding Research; AlemaniaFil: Krajewski, Pawel. Polish Academy of Sciences; PoloniaFil: Coupland, George. Max Planck Institute for Plant Breeding Research; AlemaniaBioMed Central2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10491Mateos, Julieta Lisa; Madrigal, Pedro; Tsuda, Kenichi; Rawat, Vimal; Richter, René; et al.; Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis; BioMed Central; Genome Biology; 16; 31; 2-20151465-6906enginfo:eu-repo/semantics/altIdentifier/url/https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0597-1info:eu-repo/semantics/altIdentifier/doi/10.1186/s13059-015-0597-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:22Zoai:ri.conicet.gov.ar:11336/10491instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:22.718CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
title Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
spellingShingle Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
Mateos, Julieta Lisa
TRANSCRIPTION FACTOR
FLOWERING TIME
title_short Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
title_full Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
title_fullStr Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
title_full_unstemmed Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
title_sort Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis
dc.creator.none.fl_str_mv Mateos, Julieta Lisa
Madrigal, Pedro
Tsuda, Kenichi
Rawat, Vimal
Richter, René
Romera Branchat, Maida
Fornara, Fabio
Schneeberger, Korbinian
Krajewski, Pawel
Coupland, George
author Mateos, Julieta Lisa
author_facet Mateos, Julieta Lisa
Madrigal, Pedro
Tsuda, Kenichi
Rawat, Vimal
Richter, René
Romera Branchat, Maida
Fornara, Fabio
Schneeberger, Korbinian
Krajewski, Pawel
Coupland, George
author_role author
author2 Madrigal, Pedro
Tsuda, Kenichi
Rawat, Vimal
Richter, René
Romera Branchat, Maida
Fornara, Fabio
Schneeberger, Korbinian
Krajewski, Pawel
Coupland, George
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRANSCRIPTION FACTOR
FLOWERING TIME
topic TRANSCRIPTION FACTOR
FLOWERING TIME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BACKGROUND: The initiation of flowering is an important developmental transition as it marks the beginning of the reproductive phase in plants. The MADS-box transcription factors (TFs) FLOWERING LOCUS C (FLC) and SHORT VEGETATIVE PHASE (SVP) form a complex to repress the expression of genes that initiate flowering in Arabidopsis. Both TFs play a central role in the regulatory network by conferring seasonal patterns of flowering. However, their interdependence and biological relevance when acting as a complex have not been extensively studied. RESULTS: We characterized the effects of both TFs individually and as a complex on flowering initiation using transcriptome profiling and DNA-binding occupancy. We find four major clusters regulating transcriptional responses, and that DNA binding scenarios are highly affected by the presence of the cognate partner. Remarkably, we identify genes whose regulation depends exclusively on simultaneous action of both proteins, thus distinguishing between the specificity of the SVP:FLC complex and that of each TF acting individually. The downstream targets of the SVP:FLC complex include a higher proportion of genes regulating floral induction, whereas those bound by either TF independently are biased towards floral development. Many genes involved in gibberellin-related processes are bound by the SVP:FLC complex, suggesting that direct regulation of gibberellin metabolism by FLC and SVP contributes to their effects on flowering. CONCLUSIONS: The regulatory codes controlled by SVP and FLC were deciphered at the genome-wide level revealing substantial flexibility based on dependent and independent DNA binding that may contribute to variation and robustness in the regulation of flowering.
Fil: Mateos, Julieta Lisa. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Madrigal, Pedro. Polish Academy of Sciences; Polonia
Fil: Tsuda, Kenichi. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Rawat, Vimal. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Richter, René. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Romera Branchat, Maida. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Fornara, Fabio. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Schneeberger, Korbinian. Max Planck Institute for Plant Breeding Research; Alemania
Fil: Krajewski, Pawel. Polish Academy of Sciences; Polonia
Fil: Coupland, George. Max Planck Institute for Plant Breeding Research; Alemania
description BACKGROUND: The initiation of flowering is an important developmental transition as it marks the beginning of the reproductive phase in plants. The MADS-box transcription factors (TFs) FLOWERING LOCUS C (FLC) and SHORT VEGETATIVE PHASE (SVP) form a complex to repress the expression of genes that initiate flowering in Arabidopsis. Both TFs play a central role in the regulatory network by conferring seasonal patterns of flowering. However, their interdependence and biological relevance when acting as a complex have not been extensively studied. RESULTS: We characterized the effects of both TFs individually and as a complex on flowering initiation using transcriptome profiling and DNA-binding occupancy. We find four major clusters regulating transcriptional responses, and that DNA binding scenarios are highly affected by the presence of the cognate partner. Remarkably, we identify genes whose regulation depends exclusively on simultaneous action of both proteins, thus distinguishing between the specificity of the SVP:FLC complex and that of each TF acting individually. The downstream targets of the SVP:FLC complex include a higher proportion of genes regulating floral induction, whereas those bound by either TF independently are biased towards floral development. Many genes involved in gibberellin-related processes are bound by the SVP:FLC complex, suggesting that direct regulation of gibberellin metabolism by FLC and SVP contributes to their effects on flowering. CONCLUSIONS: The regulatory codes controlled by SVP and FLC were deciphered at the genome-wide level revealing substantial flexibility based on dependent and independent DNA binding that may contribute to variation and robustness in the regulation of flowering.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10491
Mateos, Julieta Lisa; Madrigal, Pedro; Tsuda, Kenichi; Rawat, Vimal; Richter, René; et al.; Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis; BioMed Central; Genome Biology; 16; 31; 2-2015
1465-6906
url http://hdl.handle.net/11336/10491
identifier_str_mv Mateos, Julieta Lisa; Madrigal, Pedro; Tsuda, Kenichi; Rawat, Vimal; Richter, René; et al.; Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis; BioMed Central; Genome Biology; 16; 31; 2-2015
1465-6906
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0597-1
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13059-015-0597-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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