Molecular variability of watermelon mosaic virus isolates from Argentina

Autores
Pozzi, Elizabeth Alicia; Perotto, Maria Cecilia; Bertin, S.; Manglli, A.; Luciani, Cecilia Elizabeth; Conci, Vilma Cecilia; Tomassoli, L.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Watermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-CP region and compared them with isolates previously reported from around the world. Forty-six WMV isolates were obtained from naturally infected cucurbit crops collected from 10 provinces between 2011 and 2018. At the molecular level, WMV isolates were grouped into three distinct major phylogenetic groups based on genetic distance. Majority of the Argentine isolates belonged to the emerging group (G3), whereas one isolate was included in G1 and another cluster in G2. G3 was further divided into 5 subgroups, named EM1, EM2, EM3, EM4, and a new group “EMArg”, composed exclusively of Argentine isolates with high bootstrap support and high level of significance. Tajima’s D and Fu were significantly negative for G3 and EMArg, indicating that the population of this subgroup has recently expanded. AMOVA analysis showed that the isolates was not well correlated with their geographic origin. The fixation index (FST) value between the WMV isolates from the different provinces in Argentina was lower than 0.33, indicating a relatively frequent gene flow between provinces. In every group, the predominant evolutionary pressure was negative with a mean dN/dS < 1, suggesting a slow replacement fixation rate. One recombination event was detected involving isolates of EM2 cluster from Argentina.
Instituto de Patología Vegetal
Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bertin, S. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia
Fil: Manglli, A. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia
Fil: Luciani, Cecilia E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomassoli, L. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia
Fuente
European Journal of Plant Pathology (2020)
Materia
Sandia
Enfermedades de las Plantas
Variación Genética
Argentina
Watermelons
Plant Diseases
Watermelon Mosaic Virus
Genetic Variation
Virus del Mosaico de la Sandia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7065
Acceder
id INTADig_90a05c6910244155403c05b87990c934
oai_identifier_str oai:localhost:20.500.12123/7065
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular variability of watermelon mosaic virus isolates from ArgentinaPozzi, Elizabeth AliciaPerotto, Maria CeciliaBertin, S.Manglli, A.Luciani, Cecilia ElizabethConci, Vilma CeciliaTomassoli, L.SandiaEnfermedades de las PlantasVariación GenéticaArgentinaWatermelonsPlant DiseasesWatermelon Mosaic VirusGenetic VariationVirus del Mosaico de la SandiaWatermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-CP region and compared them with isolates previously reported from around the world. Forty-six WMV isolates were obtained from naturally infected cucurbit crops collected from 10 provinces between 2011 and 2018. At the molecular level, WMV isolates were grouped into three distinct major phylogenetic groups based on genetic distance. Majority of the Argentine isolates belonged to the emerging group (G3), whereas one isolate was included in G1 and another cluster in G2. G3 was further divided into 5 subgroups, named EM1, EM2, EM3, EM4, and a new group “EMArg”, composed exclusively of Argentine isolates with high bootstrap support and high level of significance. Tajima’s D and Fu were significantly negative for G3 and EMArg, indicating that the population of this subgroup has recently expanded. AMOVA analysis showed that the isolates was not well correlated with their geographic origin. The fixation index (FST) value between the WMV isolates from the different provinces in Argentina was lower than 0.33, indicating a relatively frequent gene flow between provinces. In every group, the predominant evolutionary pressure was negative with a mean dN/dS < 1, suggesting a slow replacement fixation rate. One recombination event was detected involving isolates of EM2 cluster from Argentina.Instituto de Patología VegetalFil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bertin, S. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; ItaliaFil: Manglli, A. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; ItaliaFil: Luciani, Cecilia E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tomassoli, L. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; ItaliaSpringer2020-04-09T14:21:32Z2020-04-09T14:21:32Z2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7065https://link.springer.com/article/10.1007/s10658-020-01965-30929-18731573-8469https://doi.org/10.1007/s10658-020-01965-3European Journal of Plant Pathology (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-06T09:40:21Zoai:localhost:20.500.12123/7065instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:40:21.944INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular variability of watermelon mosaic virus isolates from Argentina
title Molecular variability of watermelon mosaic virus isolates from Argentina
spellingShingle Molecular variability of watermelon mosaic virus isolates from Argentina
Pozzi, Elizabeth Alicia
Sandia
Enfermedades de las Plantas
Variación Genética
Argentina
Watermelons
Plant Diseases
Watermelon Mosaic Virus
Genetic Variation
Virus del Mosaico de la Sandia
title_short Molecular variability of watermelon mosaic virus isolates from Argentina
title_full Molecular variability of watermelon mosaic virus isolates from Argentina
title_fullStr Molecular variability of watermelon mosaic virus isolates from Argentina
title_full_unstemmed Molecular variability of watermelon mosaic virus isolates from Argentina
title_sort Molecular variability of watermelon mosaic virus isolates from Argentina
dc.creator.none.fl_str_mv Pozzi, Elizabeth Alicia
Perotto, Maria Cecilia
Bertin, S.
Manglli, A.
Luciani, Cecilia Elizabeth
Conci, Vilma Cecilia
Tomassoli, L.
author Pozzi, Elizabeth Alicia
author_facet Pozzi, Elizabeth Alicia
Perotto, Maria Cecilia
Bertin, S.
Manglli, A.
Luciani, Cecilia Elizabeth
Conci, Vilma Cecilia
Tomassoli, L.
author_role author
author2 Perotto, Maria Cecilia
Bertin, S.
Manglli, A.
Luciani, Cecilia Elizabeth
Conci, Vilma Cecilia
Tomassoli, L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Sandia
Enfermedades de las Plantas
Variación Genética
Argentina
Watermelons
Plant Diseases
Watermelon Mosaic Virus
Genetic Variation
Virus del Mosaico de la Sandia
topic Sandia
Enfermedades de las Plantas
Variación Genética
Argentina
Watermelons
Plant Diseases
Watermelon Mosaic Virus
Genetic Variation
Virus del Mosaico de la Sandia
dc.description.none.fl_txt_mv Watermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-CP region and compared them with isolates previously reported from around the world. Forty-six WMV isolates were obtained from naturally infected cucurbit crops collected from 10 provinces between 2011 and 2018. At the molecular level, WMV isolates were grouped into three distinct major phylogenetic groups based on genetic distance. Majority of the Argentine isolates belonged to the emerging group (G3), whereas one isolate was included in G1 and another cluster in G2. G3 was further divided into 5 subgroups, named EM1, EM2, EM3, EM4, and a new group “EMArg”, composed exclusively of Argentine isolates with high bootstrap support and high level of significance. Tajima’s D and Fu were significantly negative for G3 and EMArg, indicating that the population of this subgroup has recently expanded. AMOVA analysis showed that the isolates was not well correlated with their geographic origin. The fixation index (FST) value between the WMV isolates from the different provinces in Argentina was lower than 0.33, indicating a relatively frequent gene flow between provinces. In every group, the predominant evolutionary pressure was negative with a mean dN/dS < 1, suggesting a slow replacement fixation rate. One recombination event was detected involving isolates of EM2 cluster from Argentina.
Instituto de Patología Vegetal
Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bertin, S. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia
Fil: Manglli, A. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia
Fil: Luciani, Cecilia E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomassoli, L. Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria (CREA). Centro di ricerca Difesa e Certificazione; Italia
description Watermelon mosaic virus (WMV) is an economically important virus of cucurbit crops in Argentina. The available information on genetic variability must be continuously updated. In this study, we assessed the molecular variability of WMV isolates in Argentina based on the partial sequences of the NIb-CP region and compared them with isolates previously reported from around the world. Forty-six WMV isolates were obtained from naturally infected cucurbit crops collected from 10 provinces between 2011 and 2018. At the molecular level, WMV isolates were grouped into three distinct major phylogenetic groups based on genetic distance. Majority of the Argentine isolates belonged to the emerging group (G3), whereas one isolate was included in G1 and another cluster in G2. G3 was further divided into 5 subgroups, named EM1, EM2, EM3, EM4, and a new group “EMArg”, composed exclusively of Argentine isolates with high bootstrap support and high level of significance. Tajima’s D and Fu were significantly negative for G3 and EMArg, indicating that the population of this subgroup has recently expanded. AMOVA analysis showed that the isolates was not well correlated with their geographic origin. The fixation index (FST) value between the WMV isolates from the different provinces in Argentina was lower than 0.33, indicating a relatively frequent gene flow between provinces. In every group, the predominant evolutionary pressure was negative with a mean dN/dS < 1, suggesting a slow replacement fixation rate. One recombination event was detected involving isolates of EM2 cluster from Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-09T14:21:32Z
2020-04-09T14:21:32Z
2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7065
https://link.springer.com/article/10.1007/s10658-020-01965-3
0929-1873
1573-8469
https://doi.org/10.1007/s10658-020-01965-3
url http://hdl.handle.net/20.500.12123/7065
https://link.springer.com/article/10.1007/s10658-020-01965-3
https://doi.org/10.1007/s10658-020-01965-3
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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