Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops

Autores
Maugeri Suarez, Mariel; Rodriguez, Marianela; Bejerman, Nicolas Esteban; Laguna, Irma Graciela; Rodriguez Pardina, Patricia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at −70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars.
Instituto de Patología Vegetal
Fil: Maugeri Suarez, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Viruses 17 (7) : 995. (July 2025)
Materia
Soja
Virus de las Plantas
Virus del Mosaico
Variación Genética
Argentina
Soybeans
Plant Viruses
Glycine max
Mosaic Viruses
Genetic Variation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24807
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oai_identifier_str oai:localhost:20.500.12123/24807
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spelling Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean CropsMaugeri Suarez, MarielRodriguez, MarianelaBejerman, Nicolas EstebanLaguna, Irma GracielaRodriguez Pardina, PatriciaSojaVirus de las PlantasVirus del MosaicoVariación GenéticaArgentinaSoybeansPlant VirusesGlycine maxMosaic VirusesGenetic VariationSoybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at −70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars.Instituto de Patología VegetalFil: Maugeri Suarez, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaMDPI2025-12-30T11:41:10Z2025-12-30T11:41:10Z2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24807https://www.mdpi.com/1999-4915/17/7/9951999-4915https://doi.org/10.3390/v17070995Viruses 17 (7) : 995. (July 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:01Zoai:localhost:20.500.12123/24807instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:01.693INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
title Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
spellingShingle Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
Maugeri Suarez, Mariel
Soja
Virus de las Plantas
Virus del Mosaico
Variación Genética
Argentina
Soybeans
Plant Viruses
Glycine max
Mosaic Viruses
Genetic Variation
title_short Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
title_full Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
title_fullStr Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
title_full_unstemmed Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
title_sort Biological, Molecular, and Physiological Characterization of Four Soybean Mosaic Virus Isolates Present in Argentine Soybean Crops
dc.creator.none.fl_str_mv Maugeri Suarez, Mariel
Rodriguez, Marianela
Bejerman, Nicolas Esteban
Laguna, Irma Graciela
Rodriguez Pardina, Patricia
author Maugeri Suarez, Mariel
author_facet Maugeri Suarez, Mariel
Rodriguez, Marianela
Bejerman, Nicolas Esteban
Laguna, Irma Graciela
Rodriguez Pardina, Patricia
author_role author
author2 Rodriguez, Marianela
Bejerman, Nicolas Esteban
Laguna, Irma Graciela
Rodriguez Pardina, Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Soja
Virus de las Plantas
Virus del Mosaico
Variación Genética
Argentina
Soybeans
Plant Viruses
Glycine max
Mosaic Viruses
Genetic Variation
topic Soja
Virus de las Plantas
Virus del Mosaico
Variación Genética
Argentina
Soybeans
Plant Viruses
Glycine max
Mosaic Viruses
Genetic Variation
dc.description.none.fl_txt_mv Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at −70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars.
Instituto de Patología Vegetal
Fil: Maugeri Suarez, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and significant yield losses. In Argentina, during the 1990s, three isolates were collected in Marcos Juárez (MJ), Manfredi (M), and Northwestern Argentina (NOA), along with the “Planta Vinosa” (PV) isolate, which causes severe necrosis in some cultivars. These isolates were freeze-dried and stored at −70 °C for several years. They were recovered by mechanical inoculation and biologically, molecularly, and physiologically characterized for the first time. Three of the four isolates showed low genetic divergence in the P1, CI, and CP genes. Although SMV-NOA and SMV-PV had high nucleotide sequence identity, they differed in pathogenicity, seed mottling, and transmission efficiency by seeds or aphids. SMV-NOA caused early changes in photosystem II quantum efficiency (ɸPSII) and malondialdehyde (MDA) content before symptom expression (BS). After symptom development (LS), SMV-M significantly increased MDA, total soluble sugars, and starch compared to the other isolates. Thus, early changes in ɸPSII and sugars may influence late viral symptoms. Likewise, SMV-MJ induced more severe symptoms in the susceptible Davis cultivar than in Don Mario 4800. Therefore, our results demonstrate genomic, biological, and physiological differences among SMV isolates and variable interactions of SMV-MJ with two soybean cultivars.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-30T11:41:10Z
2025-12-30T11:41:10Z
2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24807
https://www.mdpi.com/1999-4915/17/7/995
1999-4915
https://doi.org/10.3390/v17070995
url http://hdl.handle.net/20.500.12123/24807
https://www.mdpi.com/1999-4915/17/7/995
https://doi.org/10.3390/v17070995
identifier_str_mv 1999-4915
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Viruses 17 (7) : 995. (July 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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