Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis

Autores
Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C.; Cataldi, Ángel Adrián; Alito, A.; Velasco, M.; Robledo, J,; Fernandez, J.; Rosa, P. da Silva; Romano, Maria Isabel
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, in-house PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.
Fil: Suffys, P.. Fundación Oswaldo Cruz; Brasil
Fil: Palomino, J. C.. Universidad Peruana Cayetano Heredia; Perú
Fil: Cardoso Leão, S.. Universidade Federal de São Paulo; Brasil
Fil: Espitia, C.. Universidad Nacional Autónoma de México; México
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Alito, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Velasco, M.. Instituto de Salud Pública de Chile. Seccion Micobacterias y Unidad de Biología Molecular; Chile
Fil: Robledo, J,. Corporación para Investigaciones Biológicas; Colombia
Fil: Fernandez, J.. Corporación para Investigaciones Biológicas; Colombia
Fil: Rosa, P. da Silva. Fundación Oswaldo Cruz; Brasil
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71869
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71869
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosisSuffys, P.Palomino, J. C.Cardoso Leão, S.Espitia, C.Cataldi, Ángel AdriánAlito, A.Velasco, M.Robledo, J,Fernandez, J.Rosa, P. da SilvaRomano, Maria IsabelTuberculosisPcrMycobacterium TuberculosisMulticenterhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, in-house PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.Fil: Suffys, P.. Fundación Oswaldo Cruz; BrasilFil: Palomino, J. C.. Universidad Peruana Cayetano Heredia; PerúFil: Cardoso Leão, S.. Universidade Federal de São Paulo; BrasilFil: Espitia, C.. Universidad Nacional Autónoma de México; MéxicoFil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Alito, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Velasco, M.. Instituto de Salud Pública de Chile. Seccion Micobacterias y Unidad de Biología Molecular; ChileFil: Robledo, J,. Corporación para Investigaciones Biológicas; ColombiaFil: Fernandez, J.. Corporación para Investigaciones Biológicas; ColombiaFil: Rosa, P. da Silva. Fundación Oswaldo Cruz; BrasilFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaInternational Union Against Tuberculosis and Lung Disease2000-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71869Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C. ; Cataldi, Ángel Adrián; et al.; Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 4; 2-2000; 179-1831027-3719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2000/00000004/00000002/art00015%3bjsessionid=3n9f6c9hox3uf.x-ic-live-02info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:58Zoai:ri.conicet.gov.ar:11336/71869instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:59.055CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
spellingShingle Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
Suffys, P.
Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
title_short Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_full Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_fullStr Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_full_unstemmed Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_sort Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Suffys, P.
Palomino, J. C.
Cardoso Leão, S.
Espitia, C.
Cataldi, Ángel Adrián
Alito, A.
Velasco, M.
Robledo, J,
Fernandez, J.
Rosa, P. da Silva
Romano, Maria Isabel
author Suffys, P.
author_facet Suffys, P.
Palomino, J. C.
Cardoso Leão, S.
Espitia, C.
Cataldi, Ángel Adrián
Alito, A.
Velasco, M.
Robledo, J,
Fernandez, J.
Rosa, P. da Silva
Romano, Maria Isabel
author_role author
author2 Palomino, J. C.
Cardoso Leão, S.
Espitia, C.
Cataldi, Ángel Adrián
Alito, A.
Velasco, M.
Robledo, J,
Fernandez, J.
Rosa, P. da Silva
Romano, Maria Isabel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
topic Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, in-house PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.
Fil: Suffys, P.. Fundación Oswaldo Cruz; Brasil
Fil: Palomino, J. C.. Universidad Peruana Cayetano Heredia; Perú
Fil: Cardoso Leão, S.. Universidade Federal de São Paulo; Brasil
Fil: Espitia, C.. Universidad Nacional Autónoma de México; México
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Alito, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Velasco, M.. Instituto de Salud Pública de Chile. Seccion Micobacterias y Unidad de Biología Molecular; Chile
Fil: Robledo, J,. Corporación para Investigaciones Biológicas; Colombia
Fil: Fernandez, J.. Corporación para Investigaciones Biológicas; Colombia
Fil: Rosa, P. da Silva. Fundación Oswaldo Cruz; Brasil
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, in-house PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.
publishDate 2000
dc.date.none.fl_str_mv 2000-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71869
Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C. ; Cataldi, Ángel Adrián; et al.; Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 4; 2-2000; 179-183
1027-3719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71869
identifier_str_mv Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C. ; Cataldi, Ángel Adrián; et al.; Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 4; 2-2000; 179-183
1027-3719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2000/00000004/00000002/art00015%3bjsessionid=3n9f6c9hox3uf.x-ic-live-02
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270180355342336
score 13.13397

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