Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)

Autores
Molina, Cesar L.; Magalhães, Meylling M.; Rodrigues, A.C.; Taniwaki, Sueli Akemi; de Souza Silva, Sheila Oliveira; Konig, Guido Alberto; Brandão, Paulo Eduardo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Due to the COVID-19 pandemic and the uncertainty about aspects of its origin, in recent years there has been an increased interest in investigating coronaviruses in wild animals. Bats are hosts of the greatest diversity of coronaviruses to date, including the ancestors of viruses that have caused outbreaks in humans. Although in Brazil, information on coronaviruses in bats has expanded, still they remain unrepresentative. To help shed some light on this matter, we collected 175 samples from bats of different species from two Brazilian states. Here, we report the previously unknown presence of an alphacoronavirus in a bat (Molossus sp.) from Ceará. The phylogenetic analysis showed close relationships with alphacoronaviruses from Brazil and Argentina, but it was not possible to determine the subgenus or species of this virus using RNA-dependent RNA-polymerase (RdRp) domain of the nsp12 protein-coding sequence as it was distant from the specimens considered by the International Committee on Taxonomy of Viruses (ICTV). Finally, by performing High-Throughput Sequencing, we were able to find contigs mostly belonging to domains of the replicase of bat coronaviruses related to American bats of the Molossidae and Vespertilionidae families.
Instituto de Biotecnología
Fil: Molina, Cesar L. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Magalhães, Meylling M. State University of Ceará. Postgraduate Program in Veterinary Sciences. Faculty of Veterinary; Brasil
Fil: Rodrigues, A. C. Federal University of São Carlos. Postgraduate Program in Fauna Conservation (PPGCFAU). Mammalian Systematics Laboratory; Brasil
Fil: Taniwaki, Sueli Akemi. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: de Souza Silva, Sheila Oliveira. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Konig, Guido Alberto. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brandão, Paulo Eduardo. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fuente
Journal of Molecular Evolution 93 (2) : 257-266 (April 2025)
Materia
Chiroptera
Alphacoronavirus
High-throughput Sequencing
Phylogenetic Analysis
Brazil
Secuenciación de Alto Rendimiento
Análisis Filogenético
Brasil
Molossus
Murciélago
Bats
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22791
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oai_identifier_str oai:localhost:20.500.12123/22791
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network_name_str INTA Digital (INTA)
spelling Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)Molina, Cesar L.Magalhães, Meylling M.Rodrigues, A.C.Taniwaki, Sueli Akemide Souza Silva, Sheila OliveiraKonig, Guido AlbertoBrandão, Paulo EduardoChiropteraAlphacoronavirusHigh-throughput SequencingPhylogenetic AnalysisBrazilSecuenciación de Alto RendimientoAnálisis FilogenéticoBrasilMolossusMurciélagoBatsDue to the COVID-19 pandemic and the uncertainty about aspects of its origin, in recent years there has been an increased interest in investigating coronaviruses in wild animals. Bats are hosts of the greatest diversity of coronaviruses to date, including the ancestors of viruses that have caused outbreaks in humans. Although in Brazil, information on coronaviruses in bats has expanded, still they remain unrepresentative. To help shed some light on this matter, we collected 175 samples from bats of different species from two Brazilian states. Here, we report the previously unknown presence of an alphacoronavirus in a bat (Molossus sp.) from Ceará. The phylogenetic analysis showed close relationships with alphacoronaviruses from Brazil and Argentina, but it was not possible to determine the subgenus or species of this virus using RNA-dependent RNA-polymerase (RdRp) domain of the nsp12 protein-coding sequence as it was distant from the specimens considered by the International Committee on Taxonomy of Viruses (ICTV). Finally, by performing High-Throughput Sequencing, we were able to find contigs mostly belonging to domains of the replicase of bat coronaviruses related to American bats of the Molossidae and Vespertilionidae families.Instituto de BiotecnologíaFil: Molina, Cesar L. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; BrasilFil: Magalhães, Meylling M. State University of Ceará. Postgraduate Program in Veterinary Sciences. Faculty of Veterinary; BrasilFil: Rodrigues, A. C. Federal University of São Carlos. Postgraduate Program in Fauna Conservation (PPGCFAU). Mammalian Systematics Laboratory; BrasilFil: Taniwaki, Sueli Akemi. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; BrasilFil: de Souza Silva, Sheila Oliveira. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; BrasilFil: Konig, Guido Alberto. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; BrasilFil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brandão, Paulo Eduardo. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; BrasilSpringer2025-06-25T13:18:25Z2025-06-25T13:18:25Z2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22791https://link.springer.com/article/10.1007/s00239-025-10236-w1432-14320022-2844https://doi.org/10.1007/s00239-025-10236-wJournal of Molecular Evolution 93 (2) : 257-266 (April 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:22Zoai:localhost:20.500.12123/22791instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:22.615INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
title Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
spellingShingle Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
Molina, Cesar L.
Chiroptera
Alphacoronavirus
High-throughput Sequencing
Phylogenetic Analysis
Brazil
Secuenciación de Alto Rendimiento
Análisis Filogenético
Brasil
Molossus
Murciélago
Bats
title_short Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
title_full Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
title_fullStr Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
title_full_unstemmed Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
title_sort Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.)
dc.creator.none.fl_str_mv Molina, Cesar L.
Magalhães, Meylling M.
Rodrigues, A.C.
Taniwaki, Sueli Akemi
de Souza Silva, Sheila Oliveira
Konig, Guido Alberto
Brandão, Paulo Eduardo
author Molina, Cesar L.
author_facet Molina, Cesar L.
Magalhães, Meylling M.
Rodrigues, A.C.
Taniwaki, Sueli Akemi
de Souza Silva, Sheila Oliveira
Konig, Guido Alberto
Brandão, Paulo Eduardo
author_role author
author2 Magalhães, Meylling M.
Rodrigues, A.C.
Taniwaki, Sueli Akemi
de Souza Silva, Sheila Oliveira
Konig, Guido Alberto
Brandão, Paulo Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chiroptera
Alphacoronavirus
High-throughput Sequencing
Phylogenetic Analysis
Brazil
Secuenciación de Alto Rendimiento
Análisis Filogenético
Brasil
Molossus
Murciélago
Bats
topic Chiroptera
Alphacoronavirus
High-throughput Sequencing
Phylogenetic Analysis
Brazil
Secuenciación de Alto Rendimiento
Análisis Filogenético
Brasil
Molossus
Murciélago
Bats
dc.description.none.fl_txt_mv Due to the COVID-19 pandemic and the uncertainty about aspects of its origin, in recent years there has been an increased interest in investigating coronaviruses in wild animals. Bats are hosts of the greatest diversity of coronaviruses to date, including the ancestors of viruses that have caused outbreaks in humans. Although in Brazil, information on coronaviruses in bats has expanded, still they remain unrepresentative. To help shed some light on this matter, we collected 175 samples from bats of different species from two Brazilian states. Here, we report the previously unknown presence of an alphacoronavirus in a bat (Molossus sp.) from Ceará. The phylogenetic analysis showed close relationships with alphacoronaviruses from Brazil and Argentina, but it was not possible to determine the subgenus or species of this virus using RNA-dependent RNA-polymerase (RdRp) domain of the nsp12 protein-coding sequence as it was distant from the specimens considered by the International Committee on Taxonomy of Viruses (ICTV). Finally, by performing High-Throughput Sequencing, we were able to find contigs mostly belonging to domains of the replicase of bat coronaviruses related to American bats of the Molossidae and Vespertilionidae families.
Instituto de Biotecnología
Fil: Molina, Cesar L. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Magalhães, Meylling M. State University of Ceará. Postgraduate Program in Veterinary Sciences. Faculty of Veterinary; Brasil
Fil: Rodrigues, A. C. Federal University of São Carlos. Postgraduate Program in Fauna Conservation (PPGCFAU). Mammalian Systematics Laboratory; Brasil
Fil: Taniwaki, Sueli Akemi. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: de Souza Silva, Sheila Oliveira. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Konig, Guido Alberto. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brandão, Paulo Eduardo. University of São Paulo. School of Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal Health; Brasil
description Due to the COVID-19 pandemic and the uncertainty about aspects of its origin, in recent years there has been an increased interest in investigating coronaviruses in wild animals. Bats are hosts of the greatest diversity of coronaviruses to date, including the ancestors of viruses that have caused outbreaks in humans. Although in Brazil, information on coronaviruses in bats has expanded, still they remain unrepresentative. To help shed some light on this matter, we collected 175 samples from bats of different species from two Brazilian states. Here, we report the previously unknown presence of an alphacoronavirus in a bat (Molossus sp.) from Ceará. The phylogenetic analysis showed close relationships with alphacoronaviruses from Brazil and Argentina, but it was not possible to determine the subgenus or species of this virus using RNA-dependent RNA-polymerase (RdRp) domain of the nsp12 protein-coding sequence as it was distant from the specimens considered by the International Committee on Taxonomy of Viruses (ICTV). Finally, by performing High-Throughput Sequencing, we were able to find contigs mostly belonging to domains of the replicase of bat coronaviruses related to American bats of the Molossidae and Vespertilionidae families.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-25T13:18:25Z
2025-06-25T13:18:25Z
2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22791
https://link.springer.com/article/10.1007/s00239-025-10236-w
1432-1432
0022-2844
https://doi.org/10.1007/s00239-025-10236-w
url http://hdl.handle.net/20.500.12123/22791
https://link.springer.com/article/10.1007/s00239-025-10236-w
https://doi.org/10.1007/s00239-025-10236-w
identifier_str_mv 1432-1432
0022-2844
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Molecular Evolution 93 (2) : 257-266 (April 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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