Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis

Autores
Foster, Camila Nayla; Monzon, Nolly María; Signorini Porchiett, Marcelo Lisandro; Aznar, Maria Natalia; Aguirre, Nerina Patricia; Valentini, Beatriz Susana; Vanzini, Víctor René; Novoa, María Belén
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Caprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assays are essential for use in endemic regions. This study aimed to develop and validate a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) to detect anti-B. melitensis antibodies in goats. The ciELISA was standardized with pre-dilutions of ISaBmS (1/8 and 1/300) in negative goat serum. Validation was done using 1254 serum samples from non-vaccinated goats: 843 from brucellosis-free flocks and 411 from infected flocks in Argentina. Serum samples were tested with the ciELISA, buffer plate antigen (BPA) test, fluorescence-polarized antigen (FPA) test, and complement fixation test (CFT). The optimal ciELISA cutoff was determined using a receiver operating characteristic (ROC) curve, with CFT as the gold standard. Sensitivity and specificity were evaluated using the Bayesian Latent Class Model (BLCM). The ciELISA showed sensitivity of 98.29 % (95 % CI: 96.4–99.6) and specificity of 98.62 % (95 % CI: 97.6–99.6). The kappa statistic (κ) values between ciELISA and other tests were: BPA (κ = 0.94), FPA (κ = 0.79), and CFT (κ = 0.95). The ciELISA demonstrated excellent performance, being rapid and objective, making it suitable for detecting anti-B. melitensis antibodies in goat serum.
Asociación Cooperadora INTA Rafaela. Laboratorio Brucelosis. Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (PICT 2021-INVI-809).
EEA Rafaela
Fil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Monzon, Nolly María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Aznar, María Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Aguirre, Nerina Patricia. Laboratorio Biovet. Rafaela, Santa Fe; Argentina
Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Valentini, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Vanzini, Víctor René. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fuente
Veterinary Immunology and Immunopathology 283 : 110931 (May 2025)
Materia
Brucella melitensis
Brucelosis
Anticuerpos
Caprinos
Brucellosis
Antibodies
ELISA
Goats
Bayesian Latent Class Analysis
Análisis de Clases Latentes Bayesiano
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23419
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23419
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosisFoster, Camila NaylaMonzon, Nolly MaríaSignorini Porchiett, Marcelo LisandroAznar, Maria NataliaAguirre, Nerina PatriciaValentini, Beatriz SusanaVanzini, Víctor RenéNovoa, María BelénBrucella melitensisBrucelosisAnticuerposCaprinosBrucellosisAntibodiesELISAGoatsBayesian Latent Class AnalysisAnálisis de Clases Latentes BayesianoCaprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assays are essential for use in endemic regions. This study aimed to develop and validate a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) to detect anti-B. melitensis antibodies in goats. The ciELISA was standardized with pre-dilutions of ISaBmS (1/8 and 1/300) in negative goat serum. Validation was done using 1254 serum samples from non-vaccinated goats: 843 from brucellosis-free flocks and 411 from infected flocks in Argentina. Serum samples were tested with the ciELISA, buffer plate antigen (BPA) test, fluorescence-polarized antigen (FPA) test, and complement fixation test (CFT). The optimal ciELISA cutoff was determined using a receiver operating characteristic (ROC) curve, with CFT as the gold standard. Sensitivity and specificity were evaluated using the Bayesian Latent Class Model (BLCM). The ciELISA showed sensitivity of 98.29 % (95 % CI: 96.4–99.6) and specificity of 98.62 % (95 % CI: 97.6–99.6). The kappa statistic (κ) values between ciELISA and other tests were: BPA (κ = 0.94), FPA (κ = 0.79), and CFT (κ = 0.95). The ciELISA demonstrated excellent performance, being rapid and objective, making it suitable for detecting anti-B. melitensis antibodies in goat serum.Asociación Cooperadora INTA Rafaela. Laboratorio Brucelosis. Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (PICT 2021-INVI-809).EEA RafaelaFil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Monzon, Nolly María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Aznar, María Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Aguirre, Nerina Patricia. Laboratorio Biovet. Rafaela, Santa Fe; ArgentinaFil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Valentini, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Vanzini, Víctor René. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaElsevier2025-08-14T10:04:37Z2025-08-14T10:04:37Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23419https://www.sciencedirect.com/science/article/abs/pii/S01652427250005100165-24271873-2534 (online)https://doi.org/10.1016/j.vetimm.2025.110931Veterinary Immunology and Immunopathology 283 : 110931 (May 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinarioinfo:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:31Zoai:localhost:20.500.12123/23419instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:31.583INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
title Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
spellingShingle Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
Foster, Camila Nayla
Brucella melitensis
Brucelosis
Anticuerpos
Caprinos
Brucellosis
Antibodies
ELISA
Goats
Bayesian Latent Class Analysis
Análisis de Clases Latentes Bayesiano
title_short Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
title_full Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
title_fullStr Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
title_full_unstemmed Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
title_sort Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
dc.creator.none.fl_str_mv Foster, Camila Nayla
Monzon, Nolly María
Signorini Porchiett, Marcelo Lisandro
Aznar, Maria Natalia
Aguirre, Nerina Patricia
Valentini, Beatriz Susana
Vanzini, Víctor René
Novoa, María Belén
author Foster, Camila Nayla
author_facet Foster, Camila Nayla
Monzon, Nolly María
Signorini Porchiett, Marcelo Lisandro
Aznar, Maria Natalia
Aguirre, Nerina Patricia
Valentini, Beatriz Susana
Vanzini, Víctor René
Novoa, María Belén
author_role author
author2 Monzon, Nolly María
Signorini Porchiett, Marcelo Lisandro
Aznar, Maria Natalia
Aguirre, Nerina Patricia
Valentini, Beatriz Susana
Vanzini, Víctor René
Novoa, María Belén
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Brucella melitensis
Brucelosis
Anticuerpos
Caprinos
Brucellosis
Antibodies
ELISA
Goats
Bayesian Latent Class Analysis
Análisis de Clases Latentes Bayesiano
topic Brucella melitensis
Brucelosis
Anticuerpos
Caprinos
Brucellosis
Antibodies
ELISA
Goats
Bayesian Latent Class Analysis
Análisis de Clases Latentes Bayesiano
dc.description.none.fl_txt_mv Caprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assays are essential for use in endemic regions. This study aimed to develop and validate a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) to detect anti-B. melitensis antibodies in goats. The ciELISA was standardized with pre-dilutions of ISaBmS (1/8 and 1/300) in negative goat serum. Validation was done using 1254 serum samples from non-vaccinated goats: 843 from brucellosis-free flocks and 411 from infected flocks in Argentina. Serum samples were tested with the ciELISA, buffer plate antigen (BPA) test, fluorescence-polarized antigen (FPA) test, and complement fixation test (CFT). The optimal ciELISA cutoff was determined using a receiver operating characteristic (ROC) curve, with CFT as the gold standard. Sensitivity and specificity were evaluated using the Bayesian Latent Class Model (BLCM). The ciELISA showed sensitivity of 98.29 % (95 % CI: 96.4–99.6) and specificity of 98.62 % (95 % CI: 97.6–99.6). The kappa statistic (κ) values between ciELISA and other tests were: BPA (κ = 0.94), FPA (κ = 0.79), and CFT (κ = 0.95). The ciELISA demonstrated excellent performance, being rapid and objective, making it suitable for detecting anti-B. melitensis antibodies in goat serum.
Asociación Cooperadora INTA Rafaela. Laboratorio Brucelosis. Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (PICT 2021-INVI-809).
EEA Rafaela
Fil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Monzon, Nolly María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Aznar, María Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Aguirre, Nerina Patricia. Laboratorio Biovet. Rafaela, Santa Fe; Argentina
Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Valentini, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Vanzini, Víctor René. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
description Caprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assays are essential for use in endemic regions. This study aimed to develop and validate a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) to detect anti-B. melitensis antibodies in goats. The ciELISA was standardized with pre-dilutions of ISaBmS (1/8 and 1/300) in negative goat serum. Validation was done using 1254 serum samples from non-vaccinated goats: 843 from brucellosis-free flocks and 411 from infected flocks in Argentina. Serum samples were tested with the ciELISA, buffer plate antigen (BPA) test, fluorescence-polarized antigen (FPA) test, and complement fixation test (CFT). The optimal ciELISA cutoff was determined using a receiver operating characteristic (ROC) curve, with CFT as the gold standard. Sensitivity and specificity were evaluated using the Bayesian Latent Class Model (BLCM). The ciELISA showed sensitivity of 98.29 % (95 % CI: 96.4–99.6) and specificity of 98.62 % (95 % CI: 97.6–99.6). The kappa statistic (κ) values between ciELISA and other tests were: BPA (κ = 0.94), FPA (κ = 0.79), and CFT (κ = 0.95). The ciELISA demonstrated excellent performance, being rapid and objective, making it suitable for detecting anti-B. melitensis antibodies in goat serum.
publishDate 2025
dc.date.none.fl_str_mv 2025-08-14T10:04:37Z
2025-08-14T10:04:37Z
2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23419
https://www.sciencedirect.com/science/article/abs/pii/S0165242725000510
0165-2427
1873-2534 (online)
https://doi.org/10.1016/j.vetimm.2025.110931
url http://hdl.handle.net/20.500.12123/23419
https://www.sciencedirect.com/science/article/abs/pii/S0165242725000510
https://doi.org/10.1016/j.vetimm.2025.110931
identifier_str_mv 0165-2427
1873-2534 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinario
info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 283 : 110931 (May 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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