Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time

Autores
Guillemi, Eliana Carolina; Ruybal, Paula; Lia, Veronica Viviana; Gonzalez, Sergio Alberto; Lew, Sergio Eduardo; Zimmer, Patricia Andrea; Lopez Arias, Ludmila Sol; Rodríguez, José Luis; Rodriguez, Sonia Y.; Frutos, Roger; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. Here we report the development of an MLST scheme based on the study of seven genes: dnaA, ftsZ, groEl, lipA, recA, secY and sucB. Five annotated genomes (Saint Maries, Florida, Mississippi, Puerto Rico and Virginia) and 53 bovine blood samples from different world regions were analyzed. High nucleotide diversity and a large proportion of synonymous substitutions, indicative of negative selection resulted from DnaSP 5.00.02 package application. Recombination events were detected in almost all genes, this evidence together with the coexistence of more than one A. marginale strain in the same sample might suggest the superinfection phenomena as a potential source of variation. The allelic profile analysis performed through GoeBURST shown two main CC that did not support geography. In addition, the AMOVA test confirmed the occurrence of at least two main genetically divergent groups. The composition of the emergent groups reflected the impact of both historical and environmental traits on A. marginale population structure. Finally, a web-based platform “Galaxy MLST-Pipeline” was developed to automate DNA sequence editing and data analysis that together with the Data Base are freely available to users. The A. marginale MLST scheme developed here is a valuable tool with a high discrimination power, besides PCR based strategies are still the better choice for epidemiological intracellular pathogens studies. Finally, the allelic profile describe herein would contribute to uncover the mechanisms in how intracellular pathogens challenge virulence paradigm.
Instituto de Biotecnología
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Lew, Sergio Eduardo. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina
Fil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rodriguez, Jose L.. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Rodriguez, Sonia Y.. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Frutos, Roger. Centre de Coopération Internationale en Recherche Agronomique pour le Développerment; Francia
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Infection, Genetics and Evolution 30 : 186-194 (March 2015)
Materia
Enfermedades de los Animales
Anaplasma Marginale
Epidemiología
Enfermedades Transmitidas por Garrapatas
Anaplasmosis
Ganado Bovino
Animal Diseases
Epidemiology
Tickborne Diseases
Cattle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4587
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4587
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and timeGuillemi, Eliana CarolinaRuybal, PaulaLia, Veronica VivianaGonzalez, Sergio AlbertoLew, Sergio EduardoZimmer, Patricia AndreaLopez Arias, Ludmila SolRodríguez, José LuisRodriguez, Sonia Y.Frutos, RogerWilkowsky, Silvina ElizabethFarber, Marisa DianaEnfermedades de los AnimalesAnaplasma MarginaleEpidemiologíaEnfermedades Transmitidas por GarrapatasAnaplasmosisGanado BovinoAnimal DiseasesEpidemiologyTickborne DiseasesCattleBovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. Here we report the development of an MLST scheme based on the study of seven genes: dnaA, ftsZ, groEl, lipA, recA, secY and sucB. Five annotated genomes (Saint Maries, Florida, Mississippi, Puerto Rico and Virginia) and 53 bovine blood samples from different world regions were analyzed. High nucleotide diversity and a large proportion of synonymous substitutions, indicative of negative selection resulted from DnaSP 5.00.02 package application. Recombination events were detected in almost all genes, this evidence together with the coexistence of more than one A. marginale strain in the same sample might suggest the superinfection phenomena as a potential source of variation. The allelic profile analysis performed through GoeBURST shown two main CC that did not support geography. In addition, the AMOVA test confirmed the occurrence of at least two main genetically divergent groups. The composition of the emergent groups reflected the impact of both historical and environmental traits on A. marginale population structure. Finally, a web-based platform “Galaxy MLST-Pipeline” was developed to automate DNA sequence editing and data analysis that together with the Data Base are freely available to users. The A. marginale MLST scheme developed here is a valuable tool with a high discrimination power, besides PCR based strategies are still the better choice for epidemiological intracellular pathogens studies. Finally, the allelic profile describe herein would contribute to uncover the mechanisms in how intracellular pathogens challenge virulence paradigm.Instituto de BiotecnologíaFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Lew, Sergio Eduardo. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; ArgentinaFil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Rodriguez, Jose L.. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Rodriguez, Sonia Y.. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Frutos, Roger. Centre de Coopération Internationale en Recherche Agronomique pour le Développerment; FranciaFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaElsevier2019-03-13T13:12:28Z2019-03-13T13:12:28Z2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/45871567-1348https://doi.org/10.1016/j.meegid.2014.12.027Infection, Genetics and Evolution 30 : 186-194 (March 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccess2025-09-29T13:44:35Zoai:localhost:20.500.12123/4587instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:35.944INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
title Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
spellingShingle Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
Guillemi, Eliana Carolina
Enfermedades de los Animales
Anaplasma Marginale
Epidemiología
Enfermedades Transmitidas por Garrapatas
Anaplasmosis
Ganado Bovino
Animal Diseases
Epidemiology
Tickborne Diseases
Cattle
title_short Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
title_full Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
title_fullStr Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
title_full_unstemmed Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
title_sort Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
dc.creator.none.fl_str_mv Guillemi, Eliana Carolina
Ruybal, Paula
Lia, Veronica Viviana
Gonzalez, Sergio Alberto
Lew, Sergio Eduardo
Zimmer, Patricia Andrea
Lopez Arias, Ludmila Sol
Rodríguez, José Luis
Rodriguez, Sonia Y.
Frutos, Roger
Wilkowsky, Silvina Elizabeth
Farber, Marisa Diana
author Guillemi, Eliana Carolina
author_facet Guillemi, Eliana Carolina
Ruybal, Paula
Lia, Veronica Viviana
Gonzalez, Sergio Alberto
Lew, Sergio Eduardo
Zimmer, Patricia Andrea
Lopez Arias, Ludmila Sol
Rodríguez, José Luis
Rodriguez, Sonia Y.
Frutos, Roger
Wilkowsky, Silvina Elizabeth
Farber, Marisa Diana
author_role author
author2 Ruybal, Paula
Lia, Veronica Viviana
Gonzalez, Sergio Alberto
Lew, Sergio Eduardo
Zimmer, Patricia Andrea
Lopez Arias, Ludmila Sol
Rodríguez, José Luis
Rodriguez, Sonia Y.
Frutos, Roger
Wilkowsky, Silvina Elizabeth
Farber, Marisa Diana
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Anaplasma Marginale
Epidemiología
Enfermedades Transmitidas por Garrapatas
Anaplasmosis
Ganado Bovino
Animal Diseases
Epidemiology
Tickborne Diseases
Cattle
topic Enfermedades de los Animales
Anaplasma Marginale
Epidemiología
Enfermedades Transmitidas por Garrapatas
Anaplasmosis
Ganado Bovino
Animal Diseases
Epidemiology
Tickborne Diseases
Cattle
dc.description.none.fl_txt_mv Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. Here we report the development of an MLST scheme based on the study of seven genes: dnaA, ftsZ, groEl, lipA, recA, secY and sucB. Five annotated genomes (Saint Maries, Florida, Mississippi, Puerto Rico and Virginia) and 53 bovine blood samples from different world regions were analyzed. High nucleotide diversity and a large proportion of synonymous substitutions, indicative of negative selection resulted from DnaSP 5.00.02 package application. Recombination events were detected in almost all genes, this evidence together with the coexistence of more than one A. marginale strain in the same sample might suggest the superinfection phenomena as a potential source of variation. The allelic profile analysis performed through GoeBURST shown two main CC that did not support geography. In addition, the AMOVA test confirmed the occurrence of at least two main genetically divergent groups. The composition of the emergent groups reflected the impact of both historical and environmental traits on A. marginale population structure. Finally, a web-based platform “Galaxy MLST-Pipeline” was developed to automate DNA sequence editing and data analysis that together with the Data Base are freely available to users. The A. marginale MLST scheme developed here is a valuable tool with a high discrimination power, besides PCR based strategies are still the better choice for epidemiological intracellular pathogens studies. Finally, the allelic profile describe herein would contribute to uncover the mechanisms in how intracellular pathogens challenge virulence paradigm.
Instituto de Biotecnología
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Lew, Sergio Eduardo. Universidad de Buenos Aires. Facultad de Ingenieria. Instituto de Ingeniería Biomédica; Argentina
Fil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rodriguez, Jose L.. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Rodriguez, Sonia Y.. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Frutos, Roger. Centre de Coopération Internationale en Recherche Agronomique pour le Développerment; Francia
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers previously applied for A. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. Here we report the development of an MLST scheme based on the study of seven genes: dnaA, ftsZ, groEl, lipA, recA, secY and sucB. Five annotated genomes (Saint Maries, Florida, Mississippi, Puerto Rico and Virginia) and 53 bovine blood samples from different world regions were analyzed. High nucleotide diversity and a large proportion of synonymous substitutions, indicative of negative selection resulted from DnaSP 5.00.02 package application. Recombination events were detected in almost all genes, this evidence together with the coexistence of more than one A. marginale strain in the same sample might suggest the superinfection phenomena as a potential source of variation. The allelic profile analysis performed through GoeBURST shown two main CC that did not support geography. In addition, the AMOVA test confirmed the occurrence of at least two main genetically divergent groups. The composition of the emergent groups reflected the impact of both historical and environmental traits on A. marginale population structure. Finally, a web-based platform “Galaxy MLST-Pipeline” was developed to automate DNA sequence editing and data analysis that together with the Data Base are freely available to users. The A. marginale MLST scheme developed here is a valuable tool with a high discrimination power, besides PCR based strategies are still the better choice for epidemiological intracellular pathogens studies. Finally, the allelic profile describe herein would contribute to uncover the mechanisms in how intracellular pathogens challenge virulence paradigm.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
2019-03-13T13:12:28Z
2019-03-13T13:12:28Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4587
1567-1348
https://doi.org/10.1016/j.meegid.2014.12.027
url http://hdl.handle.net/20.500.12123/4587
https://doi.org/10.1016/j.meegid.2014.12.027
identifier_str_mv 1567-1348
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Infection, Genetics and Evolution 30 : 186-194 (March 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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