A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae

Autores
Ferron, François; Debat, Humberto Julio; Shannon, Ashleigh; Decroly, Etienne; Canard, Bruno
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase.
Instituto de Patología Vegetal
Fil: Ferron, François. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemania
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Shannon, Ashleigh. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia
Fil: Decroly, Etienne. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia
Fil: Canard, Bruno. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemania
Fuente
NAR Genomics and Bioinformatics 2 (1) : lqz022 (March 2020)
Materia
Coronaviridae
Nidovirales
Viruses
Genetic Markers
RNA
ARN
Marcadores Genéticos
Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8369
Acceder
id INTADig_8a85bb809b5324e11a6cc30f924357b6
oai_identifier_str oai:localhost:20.500.12123/8369
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian TobaniviridaeFerron, FrançoisDebat, Humberto JulioShannon, AshleighDecroly, EtienneCanard, BrunoCoronaviridaeNidoviralesVirusesGenetic MarkersRNAARNMarcadores GenéticosVirusThe order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase.Instituto de Patología VegetalFil: Ferron, François. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; AlemaniaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); ArgentinaFil: Shannon, Ashleigh. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; FranciaFil: Decroly, Etienne. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; FranciaFil: Canard, Bruno. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; AlemaniaOxford University Press (OUP)2020-12-02T17:17:47Z2020-12-02T17:17:47Z2019-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8369https://academic.oup.com/nargab/article/2/1/lqz022/56807252631-9268 (online)https://doi.org/10.1093/nargab/lqz022NAR Genomics and Bioinformatics 2 (1) : lqz022 (March 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:58Zoai:localhost:20.500.12123/8369instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:58.28INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
title A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
spellingShingle A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
Ferron, François
Coronaviridae
Nidovirales
Viruses
Genetic Markers
RNA
ARN
Marcadores Genéticos
Virus
title_short A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
title_full A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
title_fullStr A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
title_full_unstemmed A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
title_sort A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
dc.creator.none.fl_str_mv Ferron, François
Debat, Humberto Julio
Shannon, Ashleigh
Decroly, Etienne
Canard, Bruno
author Ferron, François
author_facet Ferron, François
Debat, Humberto Julio
Shannon, Ashleigh
Decroly, Etienne
Canard, Bruno
author_role author
author2 Debat, Humberto Julio
Shannon, Ashleigh
Decroly, Etienne
Canard, Bruno
author2_role author
author
author
author
dc.subject.none.fl_str_mv Coronaviridae
Nidovirales
Viruses
Genetic Markers
RNA
ARN
Marcadores Genéticos
Virus
topic Coronaviridae
Nidovirales
Viruses
Genetic Markers
RNA
ARN
Marcadores Genéticos
Virus
dc.description.none.fl_txt_mv The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase.
Instituto de Patología Vegetal
Fil: Ferron, François. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemania
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina
Fil: Shannon, Ashleigh. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia
Fil: Decroly, Etienne. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia
Fil: Canard, Bruno. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemania
description The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-19
2020-12-02T17:17:47Z
2020-12-02T17:17:47Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8369
https://academic.oup.com/nargab/article/2/1/lqz022/5680725
2631-9268 (online)
https://doi.org/10.1093/nargab/lqz022
url http://hdl.handle.net/20.500.12123/8369
https://academic.oup.com/nargab/article/2/1/lqz022/5680725
https://doi.org/10.1093/nargab/lqz022
identifier_str_mv 2631-9268 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press (OUP)
publisher.none.fl_str_mv Oxford University Press (OUP)
dc.source.none.fl_str_mv NAR Genomics and Bioinformatics 2 (1) : lqz022 (March 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143530340188160
score 12.712165

Publicaciones similares