Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle

Autores
Brito, Barbara Patricia; Perez, Andrés Maximiliano; Capozzo, Alejandra Victoria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. Data analyzed consisted of 64 serum samples from FMDV A24/Cruzeiro vaccinated bovines challenged with the heterologous A/Argentina/2001 strain and evaluated for podal generalization. We computed the tests sensitivity (Se), specificity (Sp), and receiving operating characteristics (ROC) curve. The heterologous IgG1/IgG2 ratio was the most accurate test (Se = 0.71, Sp = 0.98), followed by heterologous IgG1 (Se = 0.53, Sp = 0.96), VNT (Se = 0.47, Sp = 1.00), whereas r1 accuracy was substantially low (Se = 0.41, Sp = 0.81). Because sensitivity of individual tests was limited, we argue that two or more of the tests should be used in combination to produce accurate estimates of protection.
Instituto de Virología
Fil: Brito, Bárbara Patricia. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos
Fil: Perez, Andrés Maximiliano. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Vaccine 32 (4) : 433-436 (January 2014)
Materia
Ganado Bovino
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Serología
Vacuna
Cattle
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Serology
Vaccines
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4342
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oai_identifier_str oai:localhost:20.500.12123/4342
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spelling Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattleBrito, Barbara PatriciaPerez, Andrés MaximilianoCapozzo, Alejandra VictoriaGanado BovinoEnfermedades de los AnimalesVirus Fiebre AftosaFiebre AftosaSerologíaVacunaCattleAnimal DiseasesAphthovirusFoot and Mouth DiseaseSerologyVaccinesFoot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. Data analyzed consisted of 64 serum samples from FMDV A24/Cruzeiro vaccinated bovines challenged with the heterologous A/Argentina/2001 strain and evaluated for podal generalization. We computed the tests sensitivity (Se), specificity (Sp), and receiving operating characteristics (ROC) curve. The heterologous IgG1/IgG2 ratio was the most accurate test (Se = 0.71, Sp = 0.98), followed by heterologous IgG1 (Se = 0.53, Sp = 0.96), VNT (Se = 0.47, Sp = 1.00), whereas r1 accuracy was substantially low (Se = 0.41, Sp = 0.81). Because sensitivity of individual tests was limited, we argue that two or more of the tests should be used in combination to produce accurate estimates of protection.Instituto de VirologíaFil: Brito, Bárbara Patricia. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados UnidosFil: Perez, Andrés Maximiliano. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-01-28T16:59:13Z2019-01-28T16:59:13Z2014-01-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0264410X13017052http://hdl.handle.net/20.500.12123/43420264-410Xhttps://doi.org/10.1016/j.vaccine.2013.12.007Vaccine 32 (4) : 433-436 (January 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4342instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.169INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
title Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
spellingShingle Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
Brito, Barbara Patricia
Ganado Bovino
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Serología
Vacuna
Cattle
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Serology
Vaccines
title_short Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
title_full Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
title_fullStr Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
title_full_unstemmed Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
title_sort Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
dc.creator.none.fl_str_mv Brito, Barbara Patricia
Perez, Andrés Maximiliano
Capozzo, Alejandra Victoria
author Brito, Barbara Patricia
author_facet Brito, Barbara Patricia
Perez, Andrés Maximiliano
Capozzo, Alejandra Victoria
author_role author
author2 Perez, Andrés Maximiliano
Capozzo, Alejandra Victoria
author2_role author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Serología
Vacuna
Cattle
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Serology
Vaccines
topic Ganado Bovino
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Serología
Vacuna
Cattle
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Serology
Vaccines
dc.description.none.fl_txt_mv Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. Data analyzed consisted of 64 serum samples from FMDV A24/Cruzeiro vaccinated bovines challenged with the heterologous A/Argentina/2001 strain and evaluated for podal generalization. We computed the tests sensitivity (Se), specificity (Sp), and receiving operating characteristics (ROC) curve. The heterologous IgG1/IgG2 ratio was the most accurate test (Se = 0.71, Sp = 0.98), followed by heterologous IgG1 (Se = 0.53, Sp = 0.96), VNT (Se = 0.47, Sp = 1.00), whereas r1 accuracy was substantially low (Se = 0.41, Sp = 0.81). Because sensitivity of individual tests was limited, we argue that two or more of the tests should be used in combination to produce accurate estimates of protection.
Instituto de Virología
Fil: Brito, Bárbara Patricia. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos
Fil: Perez, Andrés Maximiliano. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. Data analyzed consisted of 64 serum samples from FMDV A24/Cruzeiro vaccinated bovines challenged with the heterologous A/Argentina/2001 strain and evaluated for podal generalization. We computed the tests sensitivity (Se), specificity (Sp), and receiving operating characteristics (ROC) curve. The heterologous IgG1/IgG2 ratio was the most accurate test (Se = 0.71, Sp = 0.98), followed by heterologous IgG1 (Se = 0.53, Sp = 0.96), VNT (Se = 0.47, Sp = 1.00), whereas r1 accuracy was substantially low (Se = 0.41, Sp = 0.81). Because sensitivity of individual tests was limited, we argue that two or more of the tests should be used in combination to produce accurate estimates of protection.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-16
2019-01-28T16:59:13Z
2019-01-28T16:59:13Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0264410X13017052
http://hdl.handle.net/20.500.12123/4342
0264-410X
https://doi.org/10.1016/j.vaccine.2013.12.007
url https://www.sciencedirect.com/science/article/pii/S0264410X13017052
http://hdl.handle.net/20.500.12123/4342
https://doi.org/10.1016/j.vaccine.2013.12.007
identifier_str_mv 0264-410X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Vaccine 32 (4) : 433-436 (January 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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