Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS

Autores
Gil García, M.D.; Uclés, Samanta; Lozano Fernández, A.B.; Sosa, Alexis Lionel; Rodríguez Fernández-Alba, Amadeo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this analytical study is to develop an improved multi-residue methodology of high sensitivity and expanded scope for pesticide residue analysis in honeybee wax combs. The method was validated for 160 pesticide residues (including acaricides, insecticides, fungicides and herbicides) gas chromatography amenable and covering a wide variety of polarity and chemical structure. This method of analysis applied gas chromatography coupled to a triple quadrupole mass spectrometer for the quantitative analysis of pesticide residues. The extraction procedure applied was based QuEChERs method allowing acceptable recoveries for most of the pesticides (98%), within the range 60–120% with an associated precision (RSD) <20%, at concentration levels of MQL of 10 µg kg−1 for all pesticides with the exception of 3,5-dichloroaniline and chlordane (20 µg kg−1). The expanded uncertainty of the results was ±35% on average (coverage factor k=2 for a confidence level of 95%). The chromatographic multi-residue method was applied to determine levels of pesticide residues in 50 honeybee wax comb samples randomly collected from different apiaries in Spain. A total of 32 pesticide residues (14 insecticides/acaricides, 10 insecticides, 6 fungicides and 2 herbicides) were detected in the samples. The highest pesticide concentrations were found for those with insecticide-acaricide activity like acrinathrin, chlorfenvinphos, coumaphos and fluvalinate-tau, some of them are mainly applied in apiculture for controlling the honeybee parasite Varroa destructor. The total load of pesticide residues ranged from 69 to 1000 µg kg−1 for 40% of the analysed samples, 22% contained pesticide residues in the ranges of 1000–2000 µg kg−1, 24% between 2000 and 5000 µg kg−1 and 14% of the samples contained residues between 5000 and 9557 µg kg−1.
EEA Concordia
Fil: Gil García, M.D. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fil: Uclés, Samanta. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fil: Lozano Fernández, A.B. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fil: Sosa, Alexis Lionel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Rodríguez Fernández-Alba, Amadeo. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fuente
Talanta 163 : 54-64 (January 2017)
Materia
Abeja Melífera
Ceras
Residuos de Plaguicidas
Espectrometría de Masas
Honey Bees
Waxes
Pesticide Residues
Mass Spectrometry
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2890
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oai_identifier_str oai:localhost:20.500.12123/2890
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network_name_str INTA Digital (INTA)
spelling Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MSGil García, M.D.Uclés, SamantaLozano Fernández, A.B.Sosa, Alexis LionelRodríguez Fernández-Alba, AmadeoAbeja MelíferaCerasResiduos de PlaguicidasEspectrometría de MasasHoney BeesWaxesPesticide ResiduesMass SpectrometryThe aim of this analytical study is to develop an improved multi-residue methodology of high sensitivity and expanded scope for pesticide residue analysis in honeybee wax combs. The method was validated for 160 pesticide residues (including acaricides, insecticides, fungicides and herbicides) gas chromatography amenable and covering a wide variety of polarity and chemical structure. This method of analysis applied gas chromatography coupled to a triple quadrupole mass spectrometer for the quantitative analysis of pesticide residues. The extraction procedure applied was based QuEChERs method allowing acceptable recoveries for most of the pesticides (98%), within the range 60–120% with an associated precision (RSD) <20%, at concentration levels of MQL of 10 µg kg−1 for all pesticides with the exception of 3,5-dichloroaniline and chlordane (20 µg kg−1). The expanded uncertainty of the results was ±35% on average (coverage factor k=2 for a confidence level of 95%). The chromatographic multi-residue method was applied to determine levels of pesticide residues in 50 honeybee wax comb samples randomly collected from different apiaries in Spain. A total of 32 pesticide residues (14 insecticides/acaricides, 10 insecticides, 6 fungicides and 2 herbicides) were detected in the samples. The highest pesticide concentrations were found for those with insecticide-acaricide activity like acrinathrin, chlorfenvinphos, coumaphos and fluvalinate-tau, some of them are mainly applied in apiculture for controlling the honeybee parasite Varroa destructor. The total load of pesticide residues ranged from 69 to 1000 µg kg−1 for 40% of the analysed samples, 22% contained pesticide residues in the ranges of 1000–2000 µg kg−1, 24% between 2000 and 5000 µg kg−1 and 14% of the samples contained residues between 5000 and 9557 µg kg−1.EEA ConcordiaFil: Gil García, M.D. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; EspañaFil: Uclés, Samanta. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; EspañaFil: Lozano Fernández, A.B. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; EspañaFil: Sosa, Alexis Lionel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Rodríguez Fernández-Alba, Amadeo. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España2018-07-26T15:56:16Z2018-07-26T15:56:16Z2017-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0039914016308384?via%3Dihubhttp://hdl.handle.net/20.500.12123/28900039-9140https://doi.org/10.1016/j.talanta.2016.10.083Talanta 163 : 54-64 (January 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:22Zoai:localhost:20.500.12123/2890instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:23.181INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
title Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
spellingShingle Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
Gil García, M.D.
Abeja Melífera
Ceras
Residuos de Plaguicidas
Espectrometría de Masas
Honey Bees
Waxes
Pesticide Residues
Mass Spectrometry
title_short Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
title_full Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
title_fullStr Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
title_full_unstemmed Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
title_sort Multiresidue method for trace pesticide analysis in honeybee wax comb by GC-QqQ-MS
dc.creator.none.fl_str_mv Gil García, M.D.
Uclés, Samanta
Lozano Fernández, A.B.
Sosa, Alexis Lionel
Rodríguez Fernández-Alba, Amadeo
author Gil García, M.D.
author_facet Gil García, M.D.
Uclés, Samanta
Lozano Fernández, A.B.
Sosa, Alexis Lionel
Rodríguez Fernández-Alba, Amadeo
author_role author
author2 Uclés, Samanta
Lozano Fernández, A.B.
Sosa, Alexis Lionel
Rodríguez Fernández-Alba, Amadeo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Abeja Melífera
Ceras
Residuos de Plaguicidas
Espectrometría de Masas
Honey Bees
Waxes
Pesticide Residues
Mass Spectrometry
topic Abeja Melífera
Ceras
Residuos de Plaguicidas
Espectrometría de Masas
Honey Bees
Waxes
Pesticide Residues
Mass Spectrometry
dc.description.none.fl_txt_mv The aim of this analytical study is to develop an improved multi-residue methodology of high sensitivity and expanded scope for pesticide residue analysis in honeybee wax combs. The method was validated for 160 pesticide residues (including acaricides, insecticides, fungicides and herbicides) gas chromatography amenable and covering a wide variety of polarity and chemical structure. This method of analysis applied gas chromatography coupled to a triple quadrupole mass spectrometer for the quantitative analysis of pesticide residues. The extraction procedure applied was based QuEChERs method allowing acceptable recoveries for most of the pesticides (98%), within the range 60–120% with an associated precision (RSD) <20%, at concentration levels of MQL of 10 µg kg−1 for all pesticides with the exception of 3,5-dichloroaniline and chlordane (20 µg kg−1). The expanded uncertainty of the results was ±35% on average (coverage factor k=2 for a confidence level of 95%). The chromatographic multi-residue method was applied to determine levels of pesticide residues in 50 honeybee wax comb samples randomly collected from different apiaries in Spain. A total of 32 pesticide residues (14 insecticides/acaricides, 10 insecticides, 6 fungicides and 2 herbicides) were detected in the samples. The highest pesticide concentrations were found for those with insecticide-acaricide activity like acrinathrin, chlorfenvinphos, coumaphos and fluvalinate-tau, some of them are mainly applied in apiculture for controlling the honeybee parasite Varroa destructor. The total load of pesticide residues ranged from 69 to 1000 µg kg−1 for 40% of the analysed samples, 22% contained pesticide residues in the ranges of 1000–2000 µg kg−1, 24% between 2000 and 5000 µg kg−1 and 14% of the samples contained residues between 5000 and 9557 µg kg−1.
EEA Concordia
Fil: Gil García, M.D. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fil: Uclés, Samanta. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fil: Lozano Fernández, A.B. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
Fil: Sosa, Alexis Lionel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Rodríguez Fernández-Alba, Amadeo. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España
description The aim of this analytical study is to develop an improved multi-residue methodology of high sensitivity and expanded scope for pesticide residue analysis in honeybee wax combs. The method was validated for 160 pesticide residues (including acaricides, insecticides, fungicides and herbicides) gas chromatography amenable and covering a wide variety of polarity and chemical structure. This method of analysis applied gas chromatography coupled to a triple quadrupole mass spectrometer for the quantitative analysis of pesticide residues. The extraction procedure applied was based QuEChERs method allowing acceptable recoveries for most of the pesticides (98%), within the range 60–120% with an associated precision (RSD) <20%, at concentration levels of MQL of 10 µg kg−1 for all pesticides with the exception of 3,5-dichloroaniline and chlordane (20 µg kg−1). The expanded uncertainty of the results was ±35% on average (coverage factor k=2 for a confidence level of 95%). The chromatographic multi-residue method was applied to determine levels of pesticide residues in 50 honeybee wax comb samples randomly collected from different apiaries in Spain. A total of 32 pesticide residues (14 insecticides/acaricides, 10 insecticides, 6 fungicides and 2 herbicides) were detected in the samples. The highest pesticide concentrations were found for those with insecticide-acaricide activity like acrinathrin, chlorfenvinphos, coumaphos and fluvalinate-tau, some of them are mainly applied in apiculture for controlling the honeybee parasite Varroa destructor. The total load of pesticide residues ranged from 69 to 1000 µg kg−1 for 40% of the analysed samples, 22% contained pesticide residues in the ranges of 1000–2000 µg kg−1, 24% between 2000 and 5000 µg kg−1 and 14% of the samples contained residues between 5000 and 9557 µg kg−1.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-15
2018-07-26T15:56:16Z
2018-07-26T15:56:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0039914016308384?via%3Dihub
http://hdl.handle.net/20.500.12123/2890
0039-9140
https://doi.org/10.1016/j.talanta.2016.10.083
url https://www.sciencedirect.com/science/article/pii/S0039914016308384?via%3Dihub
http://hdl.handle.net/20.500.12123/2890
https://doi.org/10.1016/j.talanta.2016.10.083
identifier_str_mv 0039-9140
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Talanta 163 : 54-64 (January 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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