A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography

Autores
Micheloni, Oscar Bernardo; Ramallo, Ivana Ayelen; Christeler, Bernardo; Farroni, Abel Eduardo; Furlan, Ricardo Luis Eugenio
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing the antioxidant properties of complex natural extracts requires simple, cost-effective tools. The cupric reducing antioxidant capacity (CUPRAC) method is a reference for evaluating plant extract antioxidant capacity. A new CUPRAC thin-layer chromatography (TLC) assay, involving agarose immobilized reagents, was developed and applied to investigate the antioxidant properties of Solanum sisymbriifolium Lam., Sinapis arvensis L., and Cichorium intybus L. The best results were obtained by pouring a solution containing agarose, copper(II) chloride, 2,2-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS), and neocuproine in ammonium acetate buffer over a TLC plate at 40 °C (with final concentrations of 0.485 µmoles/cm2 copper(II) chloride and 0.368 µmoles/cm2 neocuproine) and incubating for 15 min. The limits of detection and quantification for Trolox® were 0.09 and 0.30 µg, respectively.
EEA Pergamino
Fil: Micheloni, O.B. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales. Departamento de Ciencias Básicas; Argentina
Fil: Ramallo, I. A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina
Fil: Ramallo, I. A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Christeler, Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; Argentina
Fil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelo y Agua; Argentina
Fil: Farroni, Abel Eduardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y ambientales; Argentina
Fil: Furlan, Ricardo Luis Eugenio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina
Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
JPC – Journal of Planar Chromatography – Modern TLC : 1-9. (January 2024).
Materia
Productos de Origen Vegetal
Procesamiento de Alimentos
Mejoramiento Nutricional
Antioxidantes
Extractos
Carroñeros
Plant Products
Food Processing
Nutrient Improvement
Antioxidants
Extracts
Scavengers
Autography
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16595
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16595
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatographyMicheloni, Oscar BernardoRamallo, Ivana AyelenChristeler, BernardoFarroni, Abel EduardoFurlan, Ricardo Luis EugenioProductos de Origen VegetalProcesamiento de AlimentosMejoramiento NutricionalAntioxidantesExtractosCarroñerosPlant ProductsFood ProcessingNutrient ImprovementAntioxidantsExtractsScavengersAutographyThe use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing the antioxidant properties of complex natural extracts requires simple, cost-effective tools. The cupric reducing antioxidant capacity (CUPRAC) method is a reference for evaluating plant extract antioxidant capacity. A new CUPRAC thin-layer chromatography (TLC) assay, involving agarose immobilized reagents, was developed and applied to investigate the antioxidant properties of Solanum sisymbriifolium Lam., Sinapis arvensis L., and Cichorium intybus L. The best results were obtained by pouring a solution containing agarose, copper(II) chloride, 2,2-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS), and neocuproine in ammonium acetate buffer over a TLC plate at 40 °C (with final concentrations of 0.485 µmoles/cm2 copper(II) chloride and 0.368 µmoles/cm2 neocuproine) and incubating for 15 min. The limits of detection and quantification for Trolox® were 0.09 and 0.30 µg, respectively.EEA PergaminoFil: Micheloni, O.B. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales. Departamento de Ciencias Básicas; ArgentinaFil: Ramallo, I. A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; ArgentinaFil: Ramallo, I. A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Christeler, Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; ArgentinaFil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelo y Agua; ArgentinaFil: Farroni, Abel Eduardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y ambientales; ArgentinaFil: Furlan, Ricardo Luis Eugenio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; ArgentinaFil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer Nature2024-02-14T13:52:34Z2024-02-14T13:52:34Z2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16595https://link.springer.com/article/10.1007/s00764-023-00273-w0933-41731789-0993 (online)https://doi.org/10.1007/s00764-023-00273-wJPC – Journal of Planar Chromatography – Modern TLC : 1-9. (January 2024).reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L04-I121, Diseño de alimentos funcionales y diferenciados, con énfasis en compuestos bioactivos, calidad proteica y micronutrientesinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-13T08:47:49Zoai:localhost:20.500.12123/16595instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-13 08:47:49.511INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
title A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
spellingShingle A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
Micheloni, Oscar Bernardo
Productos de Origen Vegetal
Procesamiento de Alimentos
Mejoramiento Nutricional
Antioxidantes
Extractos
Carroñeros
Plant Products
Food Processing
Nutrient Improvement
Antioxidants
Extracts
Scavengers
Autography
title_short A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
title_full A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
title_fullStr A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
title_full_unstemmed A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
title_sort A cupric reducing antioxidant capacity assay coupled to thin‑layer chromatography
dc.creator.none.fl_str_mv Micheloni, Oscar Bernardo
Ramallo, Ivana Ayelen
Christeler, Bernardo
Farroni, Abel Eduardo
Furlan, Ricardo Luis Eugenio
author Micheloni, Oscar Bernardo
author_facet Micheloni, Oscar Bernardo
Ramallo, Ivana Ayelen
Christeler, Bernardo
Farroni, Abel Eduardo
Furlan, Ricardo Luis Eugenio
author_role author
author2 Ramallo, Ivana Ayelen
Christeler, Bernardo
Farroni, Abel Eduardo
Furlan, Ricardo Luis Eugenio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Productos de Origen Vegetal
Procesamiento de Alimentos
Mejoramiento Nutricional
Antioxidantes
Extractos
Carroñeros
Plant Products
Food Processing
Nutrient Improvement
Antioxidants
Extracts
Scavengers
Autography
topic Productos de Origen Vegetal
Procesamiento de Alimentos
Mejoramiento Nutricional
Antioxidantes
Extractos
Carroñeros
Plant Products
Food Processing
Nutrient Improvement
Antioxidants
Extracts
Scavengers
Autography
dc.description.none.fl_txt_mv The use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing the antioxidant properties of complex natural extracts requires simple, cost-effective tools. The cupric reducing antioxidant capacity (CUPRAC) method is a reference for evaluating plant extract antioxidant capacity. A new CUPRAC thin-layer chromatography (TLC) assay, involving agarose immobilized reagents, was developed and applied to investigate the antioxidant properties of Solanum sisymbriifolium Lam., Sinapis arvensis L., and Cichorium intybus L. The best results were obtained by pouring a solution containing agarose, copper(II) chloride, 2,2-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS), and neocuproine in ammonium acetate buffer over a TLC plate at 40 °C (with final concentrations of 0.485 µmoles/cm2 copper(II) chloride and 0.368 µmoles/cm2 neocuproine) and incubating for 15 min. The limits of detection and quantification for Trolox® were 0.09 and 0.30 µg, respectively.
EEA Pergamino
Fil: Micheloni, O.B. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales. Departamento de Ciencias Básicas; Argentina
Fil: Ramallo, I. A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina
Fil: Ramallo, I. A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Christeler, Bernardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Calidad de Alimentos, Suelos y Agua; Argentina
Fil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, Suelo y Agua; Argentina
Fil: Farroni, Abel Eduardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y ambientales; Argentina
Fil: Furlan, Ricardo Luis Eugenio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina
Fil: Furlan, Ricardo Luis Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The use of natural antioxidant extracts in food processing is a growing trend, aligning with increasing ecological awareness and meeting consumer demands for safe, high-quality food products. Plants play a significant role in human health owing, in part, to their antioxidant properties. Analyzing the antioxidant properties of complex natural extracts requires simple, cost-effective tools. The cupric reducing antioxidant capacity (CUPRAC) method is a reference for evaluating plant extract antioxidant capacity. A new CUPRAC thin-layer chromatography (TLC) assay, involving agarose immobilized reagents, was developed and applied to investigate the antioxidant properties of Solanum sisymbriifolium Lam., Sinapis arvensis L., and Cichorium intybus L. The best results were obtained by pouring a solution containing agarose, copper(II) chloride, 2,2-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS), and neocuproine in ammonium acetate buffer over a TLC plate at 40 °C (with final concentrations of 0.485 µmoles/cm2 copper(II) chloride and 0.368 µmoles/cm2 neocuproine) and incubating for 15 min. The limits of detection and quantification for Trolox® were 0.09 and 0.30 µg, respectively.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-14T13:52:34Z
2024-02-14T13:52:34Z
2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16595
https://link.springer.com/article/10.1007/s00764-023-00273-w
0933-4173
1789-0993 (online)
https://doi.org/10.1007/s00764-023-00273-w
url http://hdl.handle.net/20.500.12123/16595
https://link.springer.com/article/10.1007/s00764-023-00273-w
https://doi.org/10.1007/s00764-023-00273-w
identifier_str_mv 0933-4173
1789-0993 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L04-I121, Diseño de alimentos funcionales y diferenciados, con énfasis en compuestos bioactivos, calidad proteica y micronutrientes
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv JPC – Journal of Planar Chromatography – Modern TLC : 1-9. (January 2024).
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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