Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology

Autores
Tomazic, Mariela Luján; Rodriguez, Anabel Elisa; Lombardelli Joaquín Andrés; Poklepovich Caride, Tomás Javier; Garro, Carlos Javier; Galarza, Roxana Ivon; Tiranti, Karina; Florin-Christensen, Mónica; Schnittger, Leonhard
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylphosphatidylinositol (GPI) are implicated in host cell attachment and invasion and represent promising vaccine candidates. A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. Altogether, 14 putative GPI-anchored proteins could be determined of which CpH1 and CpSUB2 as well as GP60 were further characterized. Sequencing and comparison of GP60, CpH1, and CpSUB1 alleles amplified from different geographic isolates showed a high degree of conservation. All three antigens were recombinant expressed and immunoblotted using sera of 12 Cryptosporidium-infected calves sampled at age periods 1–11 and 12–28 days after birth. Specific antibody reactions against the studied antigens were detected in all analyzed calves, demonstrating their immunreactivity and expression, and recognition in vivo at an early stage of host infection. Besides the acknowledged GP60 vaccinogen, the presented reverse vaccinology approach reveals the additional vaccine candidates CpH1 and CpSUB1 for inclusion into a subunit vaccine formulation.
Instituto de Patobiología
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Lombardelli Joaquín Andrés. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina
Fil: Poklepovich Caride, Tomás Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Tiranti, Karina. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina
Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fuente
Veterinary parasitology 264 : 74-78. (15 December 2018)
Materia
Bovina
Cryptosporidium Parvum
Vacuna
Bovinae
Vaccines
Reverse Vaccinology
Glycosylphosphatidylinositol
GPI
Vaccinogen
Bovine Cryptosporidiosis
Vacunación Inversa
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4495
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4495
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinologyTomazic, Mariela LujánRodriguez, Anabel ElisaLombardelli Joaquín AndrésPoklepovich Caride, Tomás JavierGarro, Carlos JavierGalarza, Roxana IvonTiranti, KarinaFlorin-Christensen, MónicaSchnittger, LeonhardBovinaCryptosporidium ParvumVacunaBovinaeVaccinesReverse VaccinologyGlycosylphosphatidylinositolGPIVaccinogenBovine CryptosporidiosisVacunación InversaThe apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylphosphatidylinositol (GPI) are implicated in host cell attachment and invasion and represent promising vaccine candidates. A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. Altogether, 14 putative GPI-anchored proteins could be determined of which CpH1 and CpSUB2 as well as GP60 were further characterized. Sequencing and comparison of GP60, CpH1, and CpSUB1 alleles amplified from different geographic isolates showed a high degree of conservation. All three antigens were recombinant expressed and immunoblotted using sera of 12 Cryptosporidium-infected calves sampled at age periods 1–11 and 12–28 days after birth. Specific antibody reactions against the studied antigens were detected in all analyzed calves, demonstrating their immunreactivity and expression, and recognition in vivo at an early stage of host infection. Besides the acknowledged GP60 vaccinogen, the presented reverse vaccinology approach reveals the additional vaccine candidates CpH1 and CpSUB1 for inclusion into a subunit vaccine formulation.Instituto de PatobiologíaFil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Lombardelli Joaquín Andrés. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; ArgentinaFil: Poklepovich Caride, Tomás Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Tiranti, Karina. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; ArgentinaFil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaElsevier2019-02-26T13:43:24Z2019-02-26T13:43:24Z2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4495https://www.sciencedirect.com/science/article/pii/S0304401718303741?via%3Dihub0304-4017https://doi.org/10.1016/j.vetpar.2018.11.007Veterinary parasitology 264 : 74-78. (15 December 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:50Zoai:localhost:20.500.12123/4495instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:50.917INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
title Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
spellingShingle Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
Tomazic, Mariela Luján
Bovina
Cryptosporidium Parvum
Vacuna
Bovinae
Vaccines
Reverse Vaccinology
Glycosylphosphatidylinositol
GPI
Vaccinogen
Bovine Cryptosporidiosis
Vacunación Inversa
title_short Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
title_full Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
title_fullStr Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
title_full_unstemmed Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
title_sort Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology
dc.creator.none.fl_str_mv Tomazic, Mariela Luján
Rodriguez, Anabel Elisa
Lombardelli Joaquín Andrés
Poklepovich Caride, Tomás Javier
Garro, Carlos Javier
Galarza, Roxana Ivon
Tiranti, Karina
Florin-Christensen, Mónica
Schnittger, Leonhard
author Tomazic, Mariela Luján
author_facet Tomazic, Mariela Luján
Rodriguez, Anabel Elisa
Lombardelli Joaquín Andrés
Poklepovich Caride, Tomás Javier
Garro, Carlos Javier
Galarza, Roxana Ivon
Tiranti, Karina
Florin-Christensen, Mónica
Schnittger, Leonhard
author_role author
author2 Rodriguez, Anabel Elisa
Lombardelli Joaquín Andrés
Poklepovich Caride, Tomás Javier
Garro, Carlos Javier
Galarza, Roxana Ivon
Tiranti, Karina
Florin-Christensen, Mónica
Schnittger, Leonhard
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovina
Cryptosporidium Parvum
Vacuna
Bovinae
Vaccines
Reverse Vaccinology
Glycosylphosphatidylinositol
GPI
Vaccinogen
Bovine Cryptosporidiosis
Vacunación Inversa
topic Bovina
Cryptosporidium Parvum
Vacuna
Bovinae
Vaccines
Reverse Vaccinology
Glycosylphosphatidylinositol
GPI
Vaccinogen
Bovine Cryptosporidiosis
Vacunación Inversa
dc.description.none.fl_txt_mv The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylphosphatidylinositol (GPI) are implicated in host cell attachment and invasion and represent promising vaccine candidates. A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. Altogether, 14 putative GPI-anchored proteins could be determined of which CpH1 and CpSUB2 as well as GP60 were further characterized. Sequencing and comparison of GP60, CpH1, and CpSUB1 alleles amplified from different geographic isolates showed a high degree of conservation. All three antigens were recombinant expressed and immunoblotted using sera of 12 Cryptosporidium-infected calves sampled at age periods 1–11 and 12–28 days after birth. Specific antibody reactions against the studied antigens were detected in all analyzed calves, demonstrating their immunreactivity and expression, and recognition in vivo at an early stage of host infection. Besides the acknowledged GP60 vaccinogen, the presented reverse vaccinology approach reveals the additional vaccine candidates CpH1 and CpSUB1 for inclusion into a subunit vaccine formulation.
Instituto de Patobiología
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Lombardelli Joaquín Andrés. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina
Fil: Poklepovich Caride, Tomás Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Tiranti, Karina. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina
Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
description The apicomplexan protozoan Cryptosporidium parvum is an important causative agent of diarrhea of neonatal bovines. Vaccination has been proposed as an advantageous strategy against cryptosporidiosis of calves since besides protection against disease it has also the potential to prevent dissemination of infective oocysts into the environment. Antigens anchored to the parasite surface via glycosylphosphatidylinositol (GPI) are implicated in host cell attachment and invasion and represent promising vaccine candidates. A reverse vaccinology approach was employed to (i) identify the GPI-anchored proteome of C. parvum using available web-based bioinformatic tools and (ii) characterize previously unrecognized novel vaccine antigens. Altogether, 14 putative GPI-anchored proteins could be determined of which CpH1 and CpSUB2 as well as GP60 were further characterized. Sequencing and comparison of GP60, CpH1, and CpSUB1 alleles amplified from different geographic isolates showed a high degree of conservation. All three antigens were recombinant expressed and immunoblotted using sera of 12 Cryptosporidium-infected calves sampled at age periods 1–11 and 12–28 days after birth. Specific antibody reactions against the studied antigens were detected in all analyzed calves, demonstrating their immunreactivity and expression, and recognition in vivo at an early stage of host infection. Besides the acknowledged GP60 vaccinogen, the presented reverse vaccinology approach reveals the additional vaccine candidates CpH1 and CpSUB1 for inclusion into a subunit vaccine formulation.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
2019-02-26T13:43:24Z
2019-02-26T13:43:24Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4495
https://www.sciencedirect.com/science/article/pii/S0304401718303741?via%3Dihub
0304-4017
https://doi.org/10.1016/j.vetpar.2018.11.007
url http://hdl.handle.net/20.500.12123/4495
https://www.sciencedirect.com/science/article/pii/S0304401718303741?via%3Dihub
https://doi.org/10.1016/j.vetpar.2018.11.007
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary parasitology 264 : 74-78. (15 December 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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