Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant

Autores
Sánchez Puerta, María Virginia; Zubko, Mikhajlo K.; Palmer, Jeffrey D.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The structure and evolution of angiosperm mitochondrial genomes are driven by extremely high rates of recombination and rearrangement. An excellent experimental system for studying these events is offered by cybrid plants, in which parental mitochondria usually fuse and their genomes recombine. Little is known about the extent, nature and consequences of mitochondrial recombination in these plants. We conducted the first study in which the organellar genomes of a cybrid – between Nicotiana tabacum and Hyoscyamus niger – were sequenced and compared to those of its parents. This cybrid mitochondrial genome is highly recombinant, reflecting at least 30 crossovers and five gene conversions between its parental genomes. It is also surprisingly large (41% and 64% larger than the parental genomes), yet contains single alleles for 90% of mitochondrial genes. Recombination produced a remarkably chimeric cybrid mitochondrial genome and occurred entirely via homologous mechanisms involving the double-strand break repair and/or break-induced replication pathways. Retention of a single form of most genes could be advantageous to minimize intracellular incompatibilities and/or reflect neutral forces that preferentially eliminate duplicated regions. We discuss the relevance of these findings to the surprisingly frequent occurrence of horizontal gene – and genome – transfer in angiosperm mitochondrial DNAs.
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zubko, Mikhajlo K.. Manchester Metropolitan University. Faculty of Science and Engineering ; Reino Unido
Fil: Palmer, Jeffrey D.. Indiana University. Department of Biology; Estados Unidos
Materia
Homologous Recombination
Cybrid
Evolution
Solanaceae
Chimeric
Mitochondria
Mtdna
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22403
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plantSánchez Puerta, María VirginiaZubko, Mikhajlo K.Palmer, Jeffrey D.Homologous RecombinationCybridEvolutionSolanaceaeChimericMitochondriaMtdnahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The structure and evolution of angiosperm mitochondrial genomes are driven by extremely high rates of recombination and rearrangement. An excellent experimental system for studying these events is offered by cybrid plants, in which parental mitochondria usually fuse and their genomes recombine. Little is known about the extent, nature and consequences of mitochondrial recombination in these plants. We conducted the first study in which the organellar genomes of a cybrid – between Nicotiana tabacum and Hyoscyamus niger – were sequenced and compared to those of its parents. This cybrid mitochondrial genome is highly recombinant, reflecting at least 30 crossovers and five gene conversions between its parental genomes. It is also surprisingly large (41% and 64% larger than the parental genomes), yet contains single alleles for 90% of mitochondrial genes. Recombination produced a remarkably chimeric cybrid mitochondrial genome and occurred entirely via homologous mechanisms involving the double-strand break repair and/or break-induced replication pathways. Retention of a single form of most genes could be advantageous to minimize intracellular incompatibilities and/or reflect neutral forces that preferentially eliminate duplicated regions. We discuss the relevance of these findings to the surprisingly frequent occurrence of horizontal gene – and genome – transfer in angiosperm mitochondrial DNAs.Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Zubko, Mikhajlo K.. Manchester Metropolitan University. Faculty of Science and Engineering ; Reino UnidoFil: Palmer, Jeffrey D.. Indiana University. Department of Biology; Estados UnidosWiley2015-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22403Sánchez Puerta, María Virginia; Zubko, Mikhajlo K.; Palmer, Jeffrey D.; Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant; Wiley; New Phytologist; 206; 1; 28-4-2015; 381-3960028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nph.13188/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/nph.13188info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342287/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:55Zoai:ri.conicet.gov.ar:11336/22403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:55.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
title Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
spellingShingle Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
Sánchez Puerta, María Virginia
Homologous Recombination
Cybrid
Evolution
Solanaceae
Chimeric
Mitochondria
Mtdna
title_short Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
title_full Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
title_fullStr Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
title_full_unstemmed Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
title_sort Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant
dc.creator.none.fl_str_mv Sánchez Puerta, María Virginia
Zubko, Mikhajlo K.
Palmer, Jeffrey D.
author Sánchez Puerta, María Virginia
author_facet Sánchez Puerta, María Virginia
Zubko, Mikhajlo K.
Palmer, Jeffrey D.
author_role author
author2 Zubko, Mikhajlo K.
Palmer, Jeffrey D.
author2_role author
author
dc.subject.none.fl_str_mv Homologous Recombination
Cybrid
Evolution
Solanaceae
Chimeric
Mitochondria
Mtdna
topic Homologous Recombination
Cybrid
Evolution
Solanaceae
Chimeric
Mitochondria
Mtdna
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The structure and evolution of angiosperm mitochondrial genomes are driven by extremely high rates of recombination and rearrangement. An excellent experimental system for studying these events is offered by cybrid plants, in which parental mitochondria usually fuse and their genomes recombine. Little is known about the extent, nature and consequences of mitochondrial recombination in these plants. We conducted the first study in which the organellar genomes of a cybrid – between Nicotiana tabacum and Hyoscyamus niger – were sequenced and compared to those of its parents. This cybrid mitochondrial genome is highly recombinant, reflecting at least 30 crossovers and five gene conversions between its parental genomes. It is also surprisingly large (41% and 64% larger than the parental genomes), yet contains single alleles for 90% of mitochondrial genes. Recombination produced a remarkably chimeric cybrid mitochondrial genome and occurred entirely via homologous mechanisms involving the double-strand break repair and/or break-induced replication pathways. Retention of a single form of most genes could be advantageous to minimize intracellular incompatibilities and/or reflect neutral forces that preferentially eliminate duplicated regions. We discuss the relevance of these findings to the surprisingly frequent occurrence of horizontal gene – and genome – transfer in angiosperm mitochondrial DNAs.
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Zubko, Mikhajlo K.. Manchester Metropolitan University. Faculty of Science and Engineering ; Reino Unido
Fil: Palmer, Jeffrey D.. Indiana University. Department of Biology; Estados Unidos
description The structure and evolution of angiosperm mitochondrial genomes are driven by extremely high rates of recombination and rearrangement. An excellent experimental system for studying these events is offered by cybrid plants, in which parental mitochondria usually fuse and their genomes recombine. Little is known about the extent, nature and consequences of mitochondrial recombination in these plants. We conducted the first study in which the organellar genomes of a cybrid – between Nicotiana tabacum and Hyoscyamus niger – were sequenced and compared to those of its parents. This cybrid mitochondrial genome is highly recombinant, reflecting at least 30 crossovers and five gene conversions between its parental genomes. It is also surprisingly large (41% and 64% larger than the parental genomes), yet contains single alleles for 90% of mitochondrial genes. Recombination produced a remarkably chimeric cybrid mitochondrial genome and occurred entirely via homologous mechanisms involving the double-strand break repair and/or break-induced replication pathways. Retention of a single form of most genes could be advantageous to minimize intracellular incompatibilities and/or reflect neutral forces that preferentially eliminate duplicated regions. We discuss the relevance of these findings to the surprisingly frequent occurrence of horizontal gene – and genome – transfer in angiosperm mitochondrial DNAs.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22403
Sánchez Puerta, María Virginia; Zubko, Mikhajlo K.; Palmer, Jeffrey D.; Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant; Wiley; New Phytologist; 206; 1; 28-4-2015; 381-396
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22403
identifier_str_mv Sánchez Puerta, María Virginia; Zubko, Mikhajlo K.; Palmer, Jeffrey D.; Homologous recombination and retention of a single form of most genes shape the highly chimeric mitochondrial genome of a cybrid plant; Wiley; New Phytologist; 206; 1; 28-4-2015; 381-396
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/nph.13188/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.13188
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342287/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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