A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil

Autores
Torrents, Jorgelina; Morel, Nicolas; Rossner, Maria Victoria; Martinez, Norberto Claudio; Nava, Santiago
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work is to compare the sensitivity of two in vitro bioassays to detect resistant to fipronil in Argentinean populations of the cattle tick Rhipicephalus microplus. Two different larval bioassays prepared with technical grade (97%) fipronil were compared: larval immersion test (LIT) and larval packet test (LPT). Seven strains from different provinces were treated with both assays. Colonia Tabay, Colonia Benítez, Intiyaco and Quimili strains were considered resistant in both LIT and LPT bioassays. The 95% confidence intervals (IC95) for lethal concentration 50% (LC50) did not overlap with the susceptible reference strain (SRS) and all the values of RR50 obtained were higher than 2. Garabato and Federal strains were considered as susceptible for both techniques because the IC95 for the LC50 overlapped with those of the SRS and the RR50 values were lesser than 2. An ambiguous situation occurs with Reconquista strain. This strain was considered as susceptible with LPT and with incipient resistant after LIT trial. The analysis of the results indicates that both LIT and LPT trials have enough sensibility to differentiate resistant and susceptible strains, but LIT was more sensitive than LPT when the resistance is incipient.
EEA Rafaela
Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Martinez, Norberto Claudio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista. Agencia de Extensión Rural Garabato; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Veterinary Parasitology : Regional Studies and Reports; 37: 100816 (January 2023)
Materia
Rhipicephalus
Fipronil
Resistance to Acaricides
Bio-assays
Cattle
Resistencia a los Acaricidas
Ensayo Biológico
Ganado Bovino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13726
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13726
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spelling A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronilTorrents, JorgelinaMorel, NicolasRossner, Maria VictoriaMartinez, Norberto ClaudioNava, SantiagoRhipicephalusFipronilResistance to AcaricidesBio-assaysCattleResistencia a los AcaricidasEnsayo BiológicoGanado BovinoThe aim of this work is to compare the sensitivity of two in vitro bioassays to detect resistant to fipronil in Argentinean populations of the cattle tick Rhipicephalus microplus. Two different larval bioassays prepared with technical grade (97%) fipronil were compared: larval immersion test (LIT) and larval packet test (LPT). Seven strains from different provinces were treated with both assays. Colonia Tabay, Colonia Benítez, Intiyaco and Quimili strains were considered resistant in both LIT and LPT bioassays. The 95% confidence intervals (IC95) for lethal concentration 50% (LC50) did not overlap with the susceptible reference strain (SRS) and all the values of RR50 obtained were higher than 2. Garabato and Federal strains were considered as susceptible for both techniques because the IC95 for the LC50 overlapped with those of the SRS and the RR50 values were lesser than 2. An ambiguous situation occurs with Reconquista strain. This strain was considered as susceptible with LPT and with incipient resistant after LIT trial. The analysis of the results indicates that both LIT and LPT trials have enough sensibility to differentiate resistant and susceptible strains, but LIT was more sensitive than LPT when the resistance is incipient.EEA RafaelaFil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; ArgentinaFil: Martinez, Norberto Claudio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista. Agencia de Extensión Rural Garabato; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaElsevier2022-12-27T13:11:34Z2022-12-27T13:11:34Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13726https://www.sciencedirect.com/science/article/pii/S24059390220013202405-9390https://doi.org/10.1016/j.vprsr.2022.100816Veterinary Parasitology : Regional Studies and Reports; 37: 100816 (January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/restrictedAccess2025-10-23T11:18:14Zoai:localhost:20.500.12123/13726instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:14.49INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
title A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
spellingShingle A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
Torrents, Jorgelina
Rhipicephalus
Fipronil
Resistance to Acaricides
Bio-assays
Cattle
Resistencia a los Acaricidas
Ensayo Biológico
Ganado Bovino
title_short A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
title_full A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
title_fullStr A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
title_full_unstemmed A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
title_sort A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
dc.creator.none.fl_str_mv Torrents, Jorgelina
Morel, Nicolas
Rossner, Maria Victoria
Martinez, Norberto Claudio
Nava, Santiago
author Torrents, Jorgelina
author_facet Torrents, Jorgelina
Morel, Nicolas
Rossner, Maria Victoria
Martinez, Norberto Claudio
Nava, Santiago
author_role author
author2 Morel, Nicolas
Rossner, Maria Victoria
Martinez, Norberto Claudio
Nava, Santiago
author2_role author
author
author
author
dc.subject.none.fl_str_mv Rhipicephalus
Fipronil
Resistance to Acaricides
Bio-assays
Cattle
Resistencia a los Acaricidas
Ensayo Biológico
Ganado Bovino
topic Rhipicephalus
Fipronil
Resistance to Acaricides
Bio-assays
Cattle
Resistencia a los Acaricidas
Ensayo Biológico
Ganado Bovino
dc.description.none.fl_txt_mv The aim of this work is to compare the sensitivity of two in vitro bioassays to detect resistant to fipronil in Argentinean populations of the cattle tick Rhipicephalus microplus. Two different larval bioassays prepared with technical grade (97%) fipronil were compared: larval immersion test (LIT) and larval packet test (LPT). Seven strains from different provinces were treated with both assays. Colonia Tabay, Colonia Benítez, Intiyaco and Quimili strains were considered resistant in both LIT and LPT bioassays. The 95% confidence intervals (IC95) for lethal concentration 50% (LC50) did not overlap with the susceptible reference strain (SRS) and all the values of RR50 obtained were higher than 2. Garabato and Federal strains were considered as susceptible for both techniques because the IC95 for the LC50 overlapped with those of the SRS and the RR50 values were lesser than 2. An ambiguous situation occurs with Reconquista strain. This strain was considered as susceptible with LPT and with incipient resistant after LIT trial. The analysis of the results indicates that both LIT and LPT trials have enough sensibility to differentiate resistant and susceptible strains, but LIT was more sensitive than LPT when the resistance is incipient.
EEA Rafaela
Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina
Fil: Martinez, Norberto Claudio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista. Agencia de Extensión Rural Garabato; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description The aim of this work is to compare the sensitivity of two in vitro bioassays to detect resistant to fipronil in Argentinean populations of the cattle tick Rhipicephalus microplus. Two different larval bioassays prepared with technical grade (97%) fipronil were compared: larval immersion test (LIT) and larval packet test (LPT). Seven strains from different provinces were treated with both assays. Colonia Tabay, Colonia Benítez, Intiyaco and Quimili strains were considered resistant in both LIT and LPT bioassays. The 95% confidence intervals (IC95) for lethal concentration 50% (LC50) did not overlap with the susceptible reference strain (SRS) and all the values of RR50 obtained were higher than 2. Garabato and Federal strains were considered as susceptible for both techniques because the IC95 for the LC50 overlapped with those of the SRS and the RR50 values were lesser than 2. An ambiguous situation occurs with Reconquista strain. This strain was considered as susceptible with LPT and with incipient resistant after LIT trial. The analysis of the results indicates that both LIT and LPT trials have enough sensibility to differentiate resistant and susceptible strains, but LIT was more sensitive than LPT when the resistance is incipient.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-27T13:11:34Z
2022-12-27T13:11:34Z
2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13726
https://www.sciencedirect.com/science/article/pii/S2405939022001320
2405-9390
https://doi.org/10.1016/j.vprsr.2022.100816
url http://hdl.handle.net/20.500.12123/13726
https://www.sciencedirect.com/science/article/pii/S2405939022001320
https://doi.org/10.1016/j.vprsr.2022.100816
identifier_str_mv 2405-9390
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology : Regional Studies and Reports; 37: 100816 (January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846787566343290880
score 12.982451

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