Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
- Autores
- Boeri, Eduardo Jorge; Ruybal, Paula; Dominguez, María Luz; Fernandez, Natalia Mercedes; Becker, Paula; Elena, Sebastián; Escobar, Gabriela Ileana; Ayala, Sandra Marcela; Hasan, Deborah Beatriz; Trangoni, Marcos David
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.
Instituto de Biotecnología
Fil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; Argentina
Fil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; Argentina
Fil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Acta Tropica 243 : 106914 (July 2023)
- Materia
-
Brucella canis
Diversidad Genética (como Recurso)
Brucelosis
Enfermedades de los Animales
Genetic Diversity (as Resource)
Brucellosis
Animal Diseases - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/14625
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Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysisBoeri, Eduardo JorgeRuybal, PaulaDominguez, María LuzFernandez, Natalia MercedesBecker, PaulaElena, SebastiánEscobar, Gabriela IleanaAyala, Sandra MarcelaHasan, Deborah BeatrizTrangoni, Marcos DavidBrucella canisDiversidad Genética (como Recurso)BrucelosisEnfermedades de los AnimalesGenetic Diversity (as Resource)BrucellosisAnimal DiseasesBrucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.Instituto de BiotecnologíaFil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; ArgentinaFil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; ArgentinaFil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. ArgentinaFil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2023-04-28T16:02:07Z2023-04-28T16:02:07Z2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14625https://www.sciencedirect.com/science/article/pii/S0001706X230010180001-706X1873-6254https://doi.org/10.1016/j.actatropica.2023.106914Acta Tropica 243 : 106914 (July 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengLatin America .......... (general region) (World)4006155info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:11Zoai:localhost:20.500.12123/14625instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:11.527INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
title |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
spellingShingle |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis Boeri, Eduardo Jorge Brucella canis Diversidad Genética (como Recurso) Brucelosis Enfermedades de los Animales Genetic Diversity (as Resource) Brucellosis Animal Diseases |
title_short |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
title_full |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
title_fullStr |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
title_full_unstemmed |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
title_sort |
Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis |
dc.creator.none.fl_str_mv |
Boeri, Eduardo Jorge Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David |
author |
Boeri, Eduardo Jorge |
author_facet |
Boeri, Eduardo Jorge Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David |
author_role |
author |
author2 |
Ruybal, Paula Dominguez, María Luz Fernandez, Natalia Mercedes Becker, Paula Elena, Sebastián Escobar, Gabriela Ileana Ayala, Sandra Marcela Hasan, Deborah Beatriz Trangoni, Marcos David |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Brucella canis Diversidad Genética (como Recurso) Brucelosis Enfermedades de los Animales Genetic Diversity (as Resource) Brucellosis Animal Diseases |
topic |
Brucella canis Diversidad Genética (como Recurso) Brucelosis Enfermedades de los Animales Genetic Diversity (as Resource) Brucellosis Animal Diseases |
dc.description.none.fl_txt_mv |
Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity. Instituto de Biotecnología Fil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; Argentina Fil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina Fil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; Argentina Fil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-28T16:02:07Z 2023-04-28T16:02:07Z 2023-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/14625 https://www.sciencedirect.com/science/article/pii/S0001706X23001018 0001-706X 1873-6254 https://doi.org/10.1016/j.actatropica.2023.106914 |
url |
http://hdl.handle.net/20.500.12123/14625 https://www.sciencedirect.com/science/article/pii/S0001706X23001018 https://doi.org/10.1016/j.actatropica.2023.106914 |
identifier_str_mv |
0001-706X 1873-6254 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Latin America .......... (general region) (World) 4006155 |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Acta Tropica 243 : 106914 (July 2023) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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