Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis

Autores
Boeri, Eduardo Jorge; Ruybal, Paula; Dominguez, María Luz; Fernandez, Natalia Mercedes; Becker, Paula; Elena, Sebastián; Escobar, Gabriela Ileana; Ayala, Sandra Marcela; Hasan, Deborah Beatriz; Trangoni, Marcos David
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.
Instituto de Biotecnología
Fil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; Argentina
Fil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; Argentina
Fil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Acta Tropica 243 : 106914 (July 2023)
Materia
Brucella canis
Diversidad Genética (como Recurso)
Brucelosis
Enfermedades de los Animales
Genetic Diversity (as Resource)
Brucellosis
Animal Diseases
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14625

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network_name_str INTA Digital (INTA)
spelling Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysisBoeri, Eduardo JorgeRuybal, PaulaDominguez, María LuzFernandez, Natalia MercedesBecker, PaulaElena, SebastiánEscobar, Gabriela IleanaAyala, Sandra MarcelaHasan, Deborah BeatrizTrangoni, Marcos DavidBrucella canisDiversidad Genética (como Recurso)BrucelosisEnfermedades de los AnimalesGenetic Diversity (as Resource)BrucellosisAnimal DiseasesBrucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.Instituto de BiotecnologíaFil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; ArgentinaFil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; ArgentinaFil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; ArgentinaFil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. ArgentinaFil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2023-04-28T16:02:07Z2023-04-28T16:02:07Z2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14625https://www.sciencedirect.com/science/article/pii/S0001706X230010180001-706X1873-6254https://doi.org/10.1016/j.actatropica.2023.106914Acta Tropica 243 : 106914 (July 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengLatin America .......... (general region) (World)4006155info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:11Zoai:localhost:20.500.12123/14625instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:11.527INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
title Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
spellingShingle Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
Boeri, Eduardo Jorge
Brucella canis
Diversidad Genética (como Recurso)
Brucelosis
Enfermedades de los Animales
Genetic Diversity (as Resource)
Brucellosis
Animal Diseases
title_short Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
title_full Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
title_fullStr Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
title_full_unstemmed Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
title_sort Higher diversity of Brucella canis in Latin America, according to an MLVA_13 Bc analysis
dc.creator.none.fl_str_mv Boeri, Eduardo Jorge
Ruybal, Paula
Dominguez, María Luz
Fernandez, Natalia Mercedes
Becker, Paula
Elena, Sebastián
Escobar, Gabriela Ileana
Ayala, Sandra Marcela
Hasan, Deborah Beatriz
Trangoni, Marcos David
author Boeri, Eduardo Jorge
author_facet Boeri, Eduardo Jorge
Ruybal, Paula
Dominguez, María Luz
Fernandez, Natalia Mercedes
Becker, Paula
Elena, Sebastián
Escobar, Gabriela Ileana
Ayala, Sandra Marcela
Hasan, Deborah Beatriz
Trangoni, Marcos David
author_role author
author2 Ruybal, Paula
Dominguez, María Luz
Fernandez, Natalia Mercedes
Becker, Paula
Elena, Sebastián
Escobar, Gabriela Ileana
Ayala, Sandra Marcela
Hasan, Deborah Beatriz
Trangoni, Marcos David
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Brucella canis
Diversidad Genética (como Recurso)
Brucelosis
Enfermedades de los Animales
Genetic Diversity (as Resource)
Brucellosis
Animal Diseases
topic Brucella canis
Diversidad Genética (como Recurso)
Brucelosis
Enfermedades de los Animales
Genetic Diversity (as Resource)
Brucellosis
Animal Diseases
dc.description.none.fl_txt_mv Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.
Instituto de Biotecnología
Fil: Boeri, Eduardo Jorge. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Departamento de de Microbiología; Argentina
Fil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dominguez, María Luz. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Fernandez, Natalia Mercedes. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Becker, Paula. Instituto de Zoonosis Luis Pasteur. División Inmunología y Diagnóstico. Laboratorio Sección Serología y Diagnóstico; Argentina
Fil: Elena, Sebastián. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección General de Laboratorio y Control Técnico. Laboratorio de Referencia de la OIE para Brucelosis; Argentina
Fil: Escobar, Gabriela Ileana. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Ayala, Sandra Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Hasan, Deborah Beatriz. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Brucella canis is the main causative agent of canine brucellosis, which affects domestic and wild canids and leads to clinical signs and symptoms of the reproductive and locomotor systems. Owing to the scarce information on this pathogen, here we addressed the genetic diversity of the circulating strains of this species in Argentina by following an MVLA_13 Bc scheme. The analyzed sample set consisted of 101 strains of B. canis isolates collected between 2006 and 2020 from canines of the Autonomous City of Buenos Aires (CABA) and other regions of Argentina, as well as 235 isolates from North America. The analysis yielded 336 variants (Hunter–Gaston Diversity Index, HGDI equal to 1.0) showing high diversity on a global scale. The analysis of the six most variable markers also reveled high diversity and allowed further analysis regarding variant relationships. Although the diversity obtained using both schemes (all or the 6 most variable markers) was higher for the Latin American than for the North American strains, we cannot discard that this was due to biases in the sampling methodology or to the different health policies employed in these regions regarding the management of infected individuals. Altogether, the Argentine circulating strains are genetically diverse, but with no apparent geographical association. The markers used in the MLVA_13 Bc are variable and highly useful for the evaluation of outbreaks. Furthermore, the reduced panel of 6 markers (MLVA_6 Bc) proposed in this study is convenient for the study of B. canis strain diversity.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-28T16:02:07Z
2023-04-28T16:02:07Z
2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14625
https://www.sciencedirect.com/science/article/pii/S0001706X23001018
0001-706X
1873-6254
https://doi.org/10.1016/j.actatropica.2023.106914
url http://hdl.handle.net/20.500.12123/14625
https://www.sciencedirect.com/science/article/pii/S0001706X23001018
https://doi.org/10.1016/j.actatropica.2023.106914
identifier_str_mv 0001-706X
1873-6254
dc.language.none.fl_str_mv eng
language eng
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Latin America .......... (general region) (World)
4006155
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Acta Tropica 243 : 106914 (July 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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